Central Vivarium Sterilizer Installation

中央饲养室灭菌器安装

基本信息

批准号：
8711607
负责人：
JOHN N. NORTON
金额：
$ 50万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-06-01 至 2016-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8711607
关键词：
Affect Agriculture American Animal Experimentation Animal Housing Animal Model Animals Area Basic Science Biomedical Engineering Businesses Canis familiaris Cardiovascular system Commit Communicable Diseases Companions Decontamination Diagnostic Direct Costs Disease Outbreaks Ensure Equipment Floor Funding Grant Growth Hazardous Substances Health Housing Human Infection Control Investments Laboratories Laboratory Animals Life Maintenance Mechanics Movement Mus Neurosciences Operative Surgical Procedures Process Quarantine Rattus Recovery Research Research Personnel Resources Risk Rodent Sanitation Side Site Soil Sterilization Sterilization for infection control Time Translational Research Translations Travel United States National Institutes of Health Universities Update Work aged animal colony animal facility animal resource design ergonomics experience improved medical schools meetings nonhuman primate operation pathogen prevent programs repaired sound square foot water conservation

项目摘要

DESCRIPTION (provided by applicant): Duke University is committed to scientific research and its translation to improve human health, including appropriate use of animals in research. The purpose of this proposal is to replace and relocate an aged autoclave located within Duke's Central Vivarium and reallocate space to optimally provide defined quarantine/isolation and animal receiving areas, and to provide the ability to decontaminate suspect caging and equipment from satellite areas. Eighty eight per cent of all mice and rats on campus are housed in barrier areas or facilities that are specifically designed to preclude entry and spread of adventitious agents that may negatively influence research objectives, and non-rodent animals are localized primarily within the Central Vivarium. The specific aims of this proposal essentially impact all animal researchers at Duke through the quarantine and isolation of 'suspect' animals and the minimizing of cross- contamination potential of infectious animals. Funds from this renovation grant are crucial to: a) replacing a 43-year- old autoclave that is necessary for maintenance of housing for isolation and quarantine in the Central Vivarium as well as for decontamination of caging and equipment from satellite facilities when rodent infectious disease outbreaks occur and b) to provide a quarantine/isolation area that is separate from the general animal housing area. Direct costs from the National Institutes of Health provide about $120 million of funding annually to animal research at Duke. Opened in 1973, the Central Vivarium is the main campus vivarium and remains structurally and operationally sound. This 29,129 net square feet (nsf) facility is arranged on two floors: a small ground floor area houses administrative and diagnostic laboratory space, with animal space located on the first floor. The Central Vivarium can house any laboratory animal species in Duke's program and is the main site for housing and use of USDA-regulated species, including non-human primates, canines, and agricultural species. Caging and equipment are processed in the Central Vivarium cagewash area on the north side of the facility and in a single corridor operation: soiled caging and equipment are removed from the animal rooms and transported into the "dirty side" of cagewash via a single corridor, clean caging and equipment exit mechanical washers directly into the clean side of cagewash. Construction funded through C06 grants from the NIH utilizing American Reinvestment and Recovery Act (ARRA) resources is underway to build 2,731 nsf to meet the needs of biomedical engineering researchers that require specialized procedural areas and large animal models as well as a two-story addition to the Central Vivarium of about 19,000 nsf. The latter project will provide housing and procedural space for USDA-regulated species (e.g., nonhuman primates, agricultural and companion species) to accommodate the growth Duke is experiencing in neuroscience, cardiovascular, surgical, biomedical engineering, and translational research. The Central Vivarium also supports a number of rodent satellite facilities that lack the ability to sanitize caging and equipment as well as autoclaving of these items. Caging and equipment from these facilities are transported to the Central Vivarium for decontamination via the sterilizer and subsequent sanitation in the cagewash area. The acquisition and relocation of a new sterilizer, along with refurbishment of an area for quarantine/isolation will improve decontamination of materials, reduce the chance of adventitious agent outbreaks, and enhance the connections of the original Central Vivarium to the C06 grant-funded Central Vivarium additions currently underway.

描述（由申请人提供）：杜克大学致力于科学研究及其转化，以改善人类健康，包括在研究中适当使用动物。该提案的目的是更换和重新安置位于杜克大学中央动物园内的老化高压灭菌器，并重新分配空间，以最佳方式提供明确的检疫/隔离和动物接收区域，并提供从卫星区域净化可疑笼子和设备的能力。校园内百分之八十八的小鼠和大鼠被安置在专门设计的屏障区域或设施中，以防止可能对研究目标产生负面影响的外来因子的进入和传播，非啮齿动物主要集中在中央动物园内。该提案的具体目标主要是通过检疫和隔离“可疑”动物以及最大限度地减少传染性动物交叉污染的可能性，影响杜克大学的所有动物研究人员。这笔翻修赠款的资金对于以下方面至关重要：a) 更换已有 43 年历史的高压灭菌器，该高压灭菌器对于维护中央动物园隔离和检疫用房以及在啮齿动物传染病发生时对卫星设施的笼子和设备进行净化是必需的b) 提供与一般动物饲养区分开的检疫/隔离区。美国国立卫生研究院的直接费用每年为杜克大学的动物研究提供约 1.2 亿美元的资金。中央动物园于 1973 年开放，是校园的主要动物园，结构和运营仍然完好。该设施占地 29,129 净平方英尺 (nsf)，分布在两层楼：一个较小的底层区域设有行政和诊断实验室空间，动物空间位于一楼。中央动物园可以容纳杜克大学项目中的任何实验动物物种，并且是容纳和使用美国农业部监管物种的主要场所，包括非人类灵长类动物、犬科动物和农业物种。笼子和设备在设施北侧的中央动物园笼洗区域和单一走廊操作中进行处理：脏笼子和设备从动物房中移出，并通过单一走廊运输到笼洗的“脏一侧”，干净的笼具和设备从机械清洗机直接进入笼具清洗的干净侧。 NIH 利用美国再投资和复苏法案 (ARRA) 资源通过 C06 拨款资助的建设正在进行中，旨在建造 2,731 nsf，以满足生物医学工程研究人员的需求，这些研究人员需要专门的程序区域和大型动物模型，以及一个两层楼的附加设施。中央动物园面积约 19,000 nsf。后一个项目将为美国农业部监管的物种（例如非人类灵长类动物、农业和伴侣物种）提供住房和程序空间，以适应杜克大学在神经科学、心血管、外科、生物医学工程和转化研究方面的发展。中央动物园还支持许多啮齿动物卫星设施，这些设施缺乏对笼子和设备进行消毒以及对这些物品进行高压灭菌的能力。这些设施的笼子和设备被运送到中央动物园，通过消毒器进行净化，并随后在笼子清洗区域进行卫生处理。购买和搬迁新灭菌器以及整修检疫/隔离区域将改善材料的净化，减少外来病原体爆发的机会，并加强原来的中央动物园与 C06 拨款资助的中央动物园的联系目前正在进行补充。