IMMUNOINCOMPETENT RODENT AND BIOHAZARD FACILITY

免疫功能低下的啮齿动物和生物危害设施

基本信息

批准号：
7764706
负责人：
JOHN N. NORTON
金额：
$ 23.78万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7764706
关键词：
Accreditation Air Air Conditioning Animals Area Biohazardous Substance Boxing Cancer Biology Cancer Center Caring Catheters Charge Containment Containment of Biohazards Doctor of Medicine Doctor of Philosophy Doctor of Veterinary Medicine Duke Comprehensive Cancer Center Ensure Equipment Fees Gnotobiotic Grant Guidelines Housing Hybridomas Image Immunochemistry Laboratories Laboratory Animals Lead Maintenance Malignant Neoplasms Mechanics Nude Mice Nude Rats Operating System Operative Surgical Procedures Positioning Attribute Procedures Production Radiolabeled Rattus Recombinant DNA Recommendation Recruitment Activity Research Personnel Research Support Resource Sharing Resources Rodent Site Visit Specific Pathogen Frees System Universities Veterinarians Work animal care animal resource authority biocontainment facility cancer research center director cost design germ free condition immunosuppressed implantation improved programs radiotracer research study square foot tumor

项目摘要

IMMUNOINCOMPETENT RODENT AND BIOHAZARD FACILITY John Norton, D.V.M., Ph.D., Director and Dareil Bigner, M.D., Ph.D., Scientific Director The Cancer Center Isoaltion Facility(CCIF) which houses the Immunoincompetent Rodent and Biocontainment Facility is the oldest shared resource in the Duke Comprehensive Cancer Center. This facility provides Cancer Center investigators with special isolated laboratories for work with hazardous biomolecules, certain recombinant DNA procedures, and for the maintenance of immunosuppressed and specific pathogen-free rodents or for other experiments that require biosafety level 2, 3, & 4 containment needed to investigate cancer biology and treatment. The building has a total of 30,000 square feet which includes eight modules housing 3 lead lined rooms, 3 horsfall rooms, 23 animal holding rooms, 9 laboratories, support areas for mechanical equipment, cage washing equipment and 26 operational autoclaves. Request for use of the space in the IRBF is evaluated by a user's committee which makes recommendations to the Director of the Cancer Center who has the authority to assign space in the building. Each investigator is assessed a fee for the use of the facility to recover the cost of staffing. The facility supports the research of 24 investigators and operates at full capacity. Dr.John N. Norton, Director of the Division of Laboratory Animal Resources and the operations of the IRBF, has the responsibility of managing and overseeing the animal care program. Dr. Darell Bigner is the scientific Coordinator of the IRBF. Mark Dewhirst, DVM, PhD will be the director of the new Animal Imaging Systems. The CCIF is staffed with 12 technicians and an operations manager who are responsible for providing care to the 4000 cages of rodents which must be maintained under barrier or containment conditions provided by the design of the building. The facility is supported by 4 fulltime mechanics paid from institutional resources to maintain and ensure the mechanical systems operate within biohazard containment guidelines. The CC1F provides space to support the Hybridoma/Immunochemistry and Radiolabeling Shared Resources.

免疫功能低下的啮齿动物和生物危害设施 John Norton，D.V.M.，Ph.D.，主任；Dareil Bigner，M.D.，Ph.D.，科学主任癌症中心隔离设施 (CCIF)，容纳免疫功能低下的啮齿动物和生物防护设施是杜克综合癌症中心最古老的共享资源。这设施为癌症中心研究人员提供特殊的隔离实验室，用于处理危险物质生物分子、某些重组 DNA 程序以及维持免疫抑制和特定的无病原体啮齿动物或需要生物安全 2、3 和 4 级遏制的其他实验需要研究癌症生物学和治疗。该建筑总面积为30,000平方英尺包括 8 个模块，包括 3 个铅衬室、3 个马棚室、23 个动物饲养室、9 个实验室、机械设备、笼式清洗设备和 26 个运行高压灭菌器的支持区域。要求 IRBF 空间的使用由用户委员会进行评估，并向用户提出建议癌症中心主任，有权分配大楼内的空间。每个调查员都是评估设施使用费以收回人员配备成本。该设施支持 24 项研究调查员并全力运作。 John N. Norton 博士，实验动物部主任 IRBF 的资源和运营，负责管理和监督动物护理程序。 Darell Bigner 博士是 IRBF 的科学协调员。 Mark Dewhirst，DVM，博士将是新动物成像系统总监。 CCIF 拥有 12 名技术人员和一名操作人员负责照顾 4000 个啮齿动物笼子的经理，这些笼子必须在建筑物设计提供的屏障或遏制条件。该设施由 4 名全职人员提供支持由机构资源支付的机械师用于维护和确保机械系统的运行符合生物危害遏制指南。 CC1F 提供空间来支持杂交瘤/免疫化学和放射性标记共享资源。