Stanford O'Brien Urology Research Center

斯坦福奥布莱恩泌尿学研究中心

• 批准号: 10297619
• 负责人: JAMES D. BROOKS
• 金额: $ 120万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-15 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10297619
• 关键词: 3-Dimensional; Affect; Atlases; Automobile Driving; Benign; Benign Prostatic Hypertrophy; Bioinformatics; Biology; CXCL13 gene; Cell Nucleus; Cell physiology; Cells; Clinical; Collaborations; Communication; Communities; Data; Data Analyses; Development; Dimensions; Disease; Elements; Embryo; Embryonic Development; Ensure; Epithelial; Epithelial Cells; Fibroblasts; Fibrosis; Functional disorder; Gene Expression; Gene Expression Profiling; Goals; Growth; Growth and Development function; Heterogeneity; Histologic; Histology; Human; Image; Immune; Immune response; Immunohistochemistry; Infiltration; Inflammation; Inflammatory; International; Investigation; Lead; Magnetic Resonance Imaging; Modeling; Molecular; Multiplexed Ion Beam Imaging; National Institute of Diabetes and Digestive and Kidney Diseases; Pathogenesis; Pathway interactions; Patients; Pilot Projects; Play; Population; Prostate; Prostatic Diseases; Prostatic Urethra; Radiology Specialty; Reporting; Research; Research Personnel; Resource Sharing; Resources; Role; Sampling; Science; Scientist; Services; Signal Pathway; Signal Transduction; Signaling Molecule; Stromal Cells; Symptoms; Technology; Testing; Therapeutic; Time; Tissue Expansion; Tissues; Training; Urologic Diseases; Urology; associated symptom; base; bioimaging; cell growth; cell type; data de-identification; experience; genomic data; graduate student; histological image; human disease; improved; indexing; lower urinary tract symptoms; man; men; molecular subtypes; next generation; novel therapeutic intervention; novel therapeutics; older men; prevent; programs; prostate enlargement; response; response to injury; senescence; success; three-dimensional modeling; transcriptomics; translational scientist; tumor; tumor-immune system interactions; undergraduate student; urinary; urologic

ABSTRACT – OVERALL COMPONENT Benign prostatic hyperplasia (BPH) is the most common cause of urinary symptoms in older men, yet we understand little about its origins, drivers of growth, and how it causes lower urinary tract symptoms. Since BPH-caused Lower Urinary Tracts Symptoms (LUTS) appears unique to man, we propose a highly integrated project to create an atlas encompassing the molecular, cellular, microenvironmental, histological and macroscopic dimensions of human BPH. Definition of the features responsible for growth and progression of BPH could ultimately lead to new therapeutic approaches to treat or prevent BPH. Our overall goal is to expand research in benign urology to improve our understanding and treatment of urological diseases. The components of the Stanford O’Brien Urology Research Center include: The Administrative Core is based in the Department of Urology and directed by Dr. James Brooks, an experienced clinician and translational scientist in prostate disease who will administer the Center to ensure the scientific and training goals are realized and interface with the NIDDK and Urology Research Consortia. He will be advised by an Internal and External Advisory Board, to ensure progress is made and to provide scientific advice to ensure success. He will meet with the Investigator Committee to formulate plans, integrate findings between projects and allocate Project and Core resources to ensure projects succeed. The Biospecimen/Bioimaging Core, directed by Dr. Robert West provides critical support to projects of the Center by providing human BPH tissues with deidentified data, generates histological images and manages these and the MRI images and provides Multiplexed Ion Beam Imaging (MIBI) and data analysis for Projects 1, 2 & 3. The Core also provides this service to the O’Brien Urology Centers and Urology Disease Centers. Project 1 seeks to define the role of fibroblast subtypes in the development and progression of BPH. Project 2 characterizes the immune microenvironment and investigates how it is shaped by the stromal cells and how it influences the stromal and epithelial compartments of BPH. Project 3 uses MR Images with associated International Prostate Symptom Scores (IPSS) and Bothersome Indices (BI) to construct 3D models of BPH overlayed with histology. These models serve as an atlas for integrating stromal and immune microenvironment data and gene expression subtypes and will provide a means to test how molecular, cellular, microenvironment, histological and radiologic features and their heterogeneity relate BPH to LUTS. These projects serve as the nucleus for training of undergraduate, graduate, and post graduate students to become the next generation of leaders in urological science.

摘要 - 整体组件 良性前列腺增生（BPH）是老年男性泌尿症状的最常见原因，但我们 了解其起源，生长驱动因素以及如何引起较低的尿路症状。自从 BPH引起的下尿路症状（LUTS）似乎是人类独特的，我们提出了一个高度整合的 项目创建一个包含分子，细胞，微环境，组织学和的地图集 人BPH的宏观维度。负责增长和发展的功能的定义 BPH最终可能导致新的治疗方法来治疗或预防BPH。我们的总体目标是 扩大良性泌尿外科的研究，以提高我们对泌尿科疾病的理解和治疗。这 斯坦福·奥布莱恩泌尿外科研究中心的组成部分包括： 行政核心位于泌尿外科，由詹姆斯·布鲁克斯博士指导， 在前列腺疾病中经验丰富的临床和翻译科学家，他们将管理该中心以确保 科学和培训目标是实现的，并与NIDDK和泌尿外科研究联盟进行了接触。他 内部和外部顾问委员会将告知，以确保取得进展并提供 科学建议以确保成功。他将与调查员委员会会面，以制定计划，整合 项目与分配项目和核心资源之间的发现，以确保项目成功。 由罗伯特·韦斯特（Robert West）执导的生物循环/生物成像核心为 通过提供人类BPH组织的中心，并产生组织学图像和管理 这些和MRI图像，并为项目1提供多路复用的离子光束成像（MIBI）和数据分析 2＆3。核心还为O'Brien泌尿外科中心和泌尿外科疾病中心提供了这项服务。 项目1旨在定义成纤维细胞亚型在BPH发展和发展中的作用。 项目2个字符免疫微环境，并研究了基质细胞如何塑造它 以及它如何影响BPH的基质和上皮室。 项目3使用具有相关国际前列腺症状分数（IPSS）和烦人的MR图像 指数（BI）构建了组织学覆盖的BPH的3D模型。这些模型是 整合基质和免疫微环境数据和基因表达亚型，并将提供 用于测试分子，细胞，微环境，组织学和放射学特征及其如何及其如何 异质性与LUTS相关的BPH。 这些项目是培训本科，研究生和研究生的核心 成为泌尿科科学领导者的下一代领导者。