Cryo-EM of Helical Protein and Nucleoprotein Polymers at Near Atomic Resolution

近原子分辨率的螺旋蛋白和核蛋白聚合物的冷冻电镜

• 批准号: 10406567
• 负责人: EDWARD H. EGELMAN
• 金额: $ 84.56万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-05-01 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10406567
• 关键词: Acids; Actins; Amyloid; Archaeal Viruses; Bacteria; Biology; Biotechnology; Computer software; Computers; Cryoelectron Microscopy; Cytoskeletal Filaments; Ebola; Electrons; Environment; Filament; Food Poisoning; Health; Human; Meningitis; Methods; Muscle; Nucleoproteins; Organism; Partner in relationship; Peptides; Pilum; Polymers; Proteins; Resolution; Structure; System; Tube; Virus; Work; detector; improved; pathogenic bacteria; small molecule; tool

Abstract We aim to greatly extend our existing studies in the proposed period, capitalizing on the great advances in electron cryo-microscopy that have been due to improved direct electron detectors, better software, and faster computers. The studies will include pili and flagellar filaments from pathogenic bacteria, mating pili, type III and IV secretion systems, and archaeal viruses infecting hosts living in the most extreme environments. In addition, we will continue studies of small molecules and peptides that self-assemble into tubes and filaments, many with anticipated biomedical applications. Our work on actin and why the sequence has been so highly conserved will continue. By working on many disparate polymers, we expect to develop more general methods that can be applied by others to helical polymers in biology. Since large numbers of such polymers exist, ranging from cytoskeletal filaments to amyloid to helical viruses (such as Ebola) the potential significance of this work is enormous and likely to have a great impact.

抽象的 我们的目标是在拟议时期大大扩展我们现有的研究，利用伟大 由于直接电子探测器的改善，电子冷冻微观显微镜的进展， 更好的软件和更快的计算机。研究将包括来自 致病细菌，交配Pili，III型和IV分泌系统以及感染的古细菌病毒 主持人生活在最极端的环境中。此外，我们将继续研究小型 分子和肽会自我组装成管子和细丝，许多人都有预期的 生物医学应用。我们在肌动蛋白上的工作以及为什么序列如此之高 将继续。通过使用许多不同的聚合物，我们希望开发更一般的聚合物 其他人可以应用于生物学的螺旋聚合物的方法。由于大量 存在聚合物，从细胞骨架丝到淀粉样蛋白到螺旋病毒（例如埃博拉病毒） 这项工作的潜在意义是巨大的，并且可能产生很大的影响。