Cryo-EM of Helical Protein and Nucleoprotein Polymers at Near Atomic Resolution

近原子分辨率的螺旋蛋白和核蛋白聚合物的冷冻电镜

• 批准号: 10793162
• 负责人: EDWARD H. EGELMAN
• 金额: $ 2.3万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-05-01 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10793162
• 关键词: Acids; Actins; Bacteria; Biotechnology; Cryoelectron Microscopy; Filament; Food Poisoning; Funding; Health; Human; Ice; Meningitis; Muscle; Negative Staining; Nucleoproteins; Organism; Polymers; Preparation; Proteins; Resolution; Sampling; Structure; Virus; cost effective; hydrophilicity; repaired; tool; Acids; Actins; Bacteria; Biotechnology; Cryoelectron Microscopy; Filament; Food Poisoning; Funding; Health; Human; Ice; Meningitis; Muscle; Negative Staining; Nucleoproteins; Organism; Polymers; Preparation; Proteins; Resolution; Sampling; Structure; Virus; cost effective; hydrophilicity; repaired; tool

Abstract Supplementary funds are requested to purchase a glow discharge apparatus to be used nearly daily for the projects funded by this R35. Glow discharge of EM grids is needed for both negative stain and cryo-EM applications to clean the grids and make them more hydrophilic. This is a very important step in sample preparation, and is crucial to getting good ice for cryo-EM. The existing apparatus was purchased in my lab ~ 13 years ago, is failing to function properly, and cannot be repaired in any cost-effective way.

抽象的 要求补充资金购买光泽放电设备，几乎每天都使用 该R35资助的项目。负污渍和冷冻EM都需要EM网格的发光排放 用于清洁网格并使它们更亲密的应用。这是样本中非常重要的一步 准备工作，对于冰冻EM获得良好的冰至关重要。现有设备是在我的实验室购买的〜 13年前，无法正常运行，无法以任何具有成本效益的方式维修。