Cryo-EM of Helical Protein and Nucleoprotein Polymers at Near Atomic Resolution

近原子分辨率的螺旋蛋白和核蛋白聚合物的冷冻电镜

• 批准号: 10153815
• 负责人: EDWARD H. EGELMAN
• 金额: $ 67.54万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-05-01 至 2022-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10153815
• 关键词: Acids; Actins; Amyloid; Bacteria; Biology; Biotechnology; Cholera; Complex; Cryoelectron Microscopy; Cytoskeletal Filaments; Ebola; Electrons; Filament; Health; Human; Meningitis; Methods; Muscle; Nucleoproteins; Organism; Pathogenesis; Pilum; Polymers; Proteins; Resolution; Structure; Type II Secretion System Pathway; Virus; Work; detector; tool

Abstract We aim to greatly extend our existing studies in the two current R01s (Actin and Nucleoprotein Complexes) in the proposed period, capitalizing on the advances in electron cryo-microscopy that have only recently become possible with direct electron detectors. In addition, we will work on a number of projects that are outside the scope of the present R01s, but involve bacterial pathogenesis and thus have great relevance to human health: Type IV pili, Type II Secretion System, bacterial and archaeal flagellar filaments. By working on many disparate polymers, we expect to develop more general methods that can be applied by others to helical polymers in biology. Since large numbers of such polymers exist, ranging from cytoskeletal filaments to amyloid to helical viruses (such as Ebola), the potential significance of this work is enormous and likely to have a great impact.

抽象的 我们的目标是在两个当前的R01中大大扩展我们的现有研究（肌动蛋白和核蛋白 复合物）在拟议的时期，利用电子冷冻微镜的进步 直到直接通过直接电子探测器才有可能。此外，我们将工作 在当前R01范围之外的许多项目上，但涉及细菌 发病机理，因此与人类健康有很大的相关性：IV pili，II型分泌 系统，细菌和古细菌鞭毛细丝。通过从事许多不同聚合物，我们 期望开发更多的通用方法，而其他人可以应用于螺旋聚合物 生物学。由于存在大量此类聚合物，因此从细胞骨架丝到 淀粉样蛋白到螺旋病毒（例如埃博拉病毒），这项工作的潜在意义是巨大的 并可能产生很大的影响。