Multispectral and Hyperspectral Preclinical Imager Spanning the Visible, NIR-I and NIR-II

跨越可见光、NIR-I 和 NIR-II 的多光谱和高光谱临床前成像仪

• 批准号: 10193523
• 负责人: David A Boas
• 金额: $ 40.43万
• 依托单位: BOSTON UNIVERSITY (CHARLES RIVER CAMPUS)
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10193523
• 关键词: Arts; Bioluminescence; Boston; Cells; Complex; Contrast Media; Custom; Development; Disease; Engineering; Equipment; Funding; Future; Health; Human; Image; Light; Microscopy; Mus; Optics; Penetration; Recovery; Research; Research Personnel; Resources; Rivers; Science; System; Techniques; Tissues; United States National Institutes of Health; Universities; base; bioluminescence imaging; cancer imaging; college; cost; design; fluorescence imaging; imager; imaging capabilities; improved; in vivo imaging system; instrument; interest; light scattering; nanomedicine; nanoparticle; optical imaging; pre-clinical; programs; whole animal imaging

Project Summary/Abstract This is a proposal from ten investigators from the College of Engineering, the College of Arts and Sciences and Sargent College at Boston University (BU) requesting purchase of a preclinical imager for photoluminescence and bioluminescence in mice. While the researchers at Boston University make extensive use of light-based optical imaging, primarily with advanced microscopy techniques on campus as well as off-campus in vivo imaging systems, there currently is no whole animal imaging capability on the BU Charles River Campus. Acquisition of a preclinical imager will allow the researchers to advance their NIH funded research programs that depend on the examination of complex, intact mammalian systems. The users propose the purchase of a first-to-market imager that will enable both traditional fluorescence and bioluminescence imaging in the visible and first near infrared (NIR-I) optical tissue window wavelength ranges (500 – 650 nm and 650 – 900 nm, respectively) as well as shortwave infrared (SWIR, also known as the second NIR optical tissue window or NIR-II) imaging. By using light in the 1000 – 1700 nm range, deeper penetration depths are achieved with less light scattering, leading to unprecedented clarity in tissue-depth light-based imaging. By designing a customized instrument to cover this extended wavelength range, the BU researchers will be able to continue current research directions that depend on traditional imaging more quickly and conveniently using local, on-campus equipment, while also hastening the funded development of SWIR contrast agents and enabling future directions using SWIR imaging that are beyond what is openly available to any researcher in the region. The users for the proposed instrument have a diversity of research interests. The proposed applications of the preclinical imager are typically focused on the tracking of engineered cells using bioluminescence, the imaging of tumors and vasculature with exogenous contrast agents, and the tracking of nanoparticles for nanomedicine applications. In this proposal, we describe the instrument we aim to acquire, the benefits of this particular instrument over others, provide a management plan for instrument use and cost recovery, and illustrate the institutional commitment to maintaining this resource. Overall, the acquisition of this preclinical imager will support projects focused on improving diverse aspects of human health and the treatment of disease.

项目摘要/摘要 这是工程学院，艺术与科学学院的十位调查人员的提议， 波士顿大学（BU）的萨金特学院要求购买光致发光的临床前成像仪 和小鼠的生物发光。波士顿大学的研究人员广泛使用基于光的 光学成像，主要采用校园的高级显微镜技术以及体内成像校园 系统，目前在Bu Charles River校园没有全动物成像能力。获取 临床前成像师将允许研究人员推进其NIH资助的研究计划，以依靠 检查复杂，完整的哺乳动物系统。用户建议购买第一到市场 成像仪将在可见的，首先启用传统的荧光和生物发光成像 红外（NIR-I）光学组织窗口波长范围（分别为500 - 650 nm和650 - 900 nm） 作为短波感染（SWIR，也称为第二个NIR光学组织窗口或NIR-II）成像。通过使用 在1000 - 1700 nm范围内的光线，具有较少光散射的深度，可实现更深的渗透深度，导致 组织深度基于光的光成像中的前所未有的清晰度。通过设计定制仪器来覆盖此 扩展波长范围，BU研究人员将能够继续进行当前的研究方向 在传统成像上更快，更方便地使用本地的校园设备，同时也加速了 SWIR对比剂的基本发展，并使用SWIR成像启用未来的方向 超越该地区任何研究人员公开可用的东西。 拟议工具的用户具有多种研究兴趣。提议的申请 临床前成像仪通常专注于使用生物发光的工程细胞跟踪，成像 具有外源对比剂的肿瘤和脉管系统，以及纳米医学的纳米颗粒的跟踪 申请。 在此提案中，我们描述了我们旨在获取的工具，该特定工具的好处 其他人，为仪器使用和成本恢复提供管理计划，并说明了机构 致力于维护此资源。总体而言，此临床前成像器的获取将支持项目 专注于改善潜水员的人类健康方面和疾病治疗。