Analytical Chemistry Core (ACC)

分析化学核心（ACC）

• 批准号: 10353157
• 负责人: P. Lee Ferguson
• 金额: $ 21.46万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2027-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10353157
• 关键词: Analytical Chemistry; Aromatic Polycyclic Hydrocarbons; Arsenic; Biodegradation; Biological; Biological Markers; Body Burden; Cadmium; Chemicals; Chromium; Collaborations; Complex; Complex Mixtures; Consult; Data; Data Analyses; Development; Dose; Ecosystem; Engineering; Environment; Evaluation; Experimental Designs; Exposure to; Flame Retardants; Funding; Hazardous Substances; Health; High Pressure Liquid Chromatography; House Dust; Human; Human Resources; Hydrophobicity; Inductively Coupled Plasma Mass Spectrometry; Instruction; Laboratory Research; Lead; Life; Liquid Chromatography; Mass Fragmentography; Mass Spectrum Analysis; Measurement; Measures; Mercury; Metabolic; Metals; Methods; Monitor; Neurotransmitters; Organic Chemicals; Organophosphates; Outcome; Pentachlorophenol; Pesticides; Polychlorinated Biphenyls; Preparation; Protocols documentation; Research; Research Activity; Research Personnel; Research Project Grants; Resolution; Resources; Role; Sampling; Schedule; Selenium; Services; Superfund; Techniques; Technology; Testing; Tissues; Toxic effect; Trace metal; Training; Universities; analytical method; base; cost; developmental toxicity; early life exposure; effectiveness evaluation; experience; hands on instruction; health assessment; instrument; instrumentation; interest; novel; organic contaminant; pollutant; remediation; service utilization; success; superfund chemical; superfund site; tandem mass spectrometry

Abstract The Duke University Superfund Research Center (DUSRC) will examine the neurodevelopmental health impacts of early-life co-exposures to PAHs and metals. The overall function of the Analytical Chemistry Core (ACC) is to provide routine sample analysis of Superfund-relevant contaminants as well as biomarkers of exposure for Research Projects within the DUSRC. Furthermore, the ACC provides analytical expertise in identifying transformation products and/or metabolites of these Superfund contaminants using high-resolution mass spectrometry techniques. The ACC has been an essential component of DUSRC over the past funding period, including the development of new analytical protocols for organic and inorganic compounds and the analysis of more than 2,000 samples for a wide variety of analytes (e.g., flame retardants, organophosphate pesticides, PAHs, and trace metals) in biological and environmental samples. Progress has been made in applying high- resolution mass spectrometry and other methods for non-targeted and effects-directed analysis of novel contaminants and transformation products of Superfund-relevant chemicals in environmental and biological media. Here, we propose to continue and extend the activities of the ACC, with a focus on targeted analysis of PAHs, metals, neurotransmitter biomarkers in biological and environmental samples as well as non-targeted and transformation product analysis of PAH compounds. In addition, we propose an increased role for the ACC to coordinate cross-project collaborations through the analysis of complex environmental mixtures (e.g., leachates of house dust, PAH biodegradation products) that will be generated by Projects 1 and 5, and shared to Project 2, 3, and 4 for evaluation of toxicity potential. The ACC will engage with the Research Experience and Training Core by leading training and consulting activities related to analytical chemistry. The ACC team will also offer informal hands-on instruction for training on sample preparation methods and instrumental analyses. We anticipate that these interactions will help guide researchers on experimental design for their research activities that will require chemical analyses. Finally, we will leverage our expertise to develop analytical methods and perform analyses of other Superfund-relevant compounds that may emerge during the experimentation of complex mixtures and study at Superfund sites. These activities will draw on our considerable success to date in developing, validating, and applying methods for contaminant analysis in environmental and biological samples.

抽象的 杜克大学超级基金研究中心 (DUSRC) 将研究神经发育健康影响 生命早期同时接触多环芳烃和金属。分析化学核心（ACC）的总体功能是 提供超级基金相关污染物的常规样本分析以及暴露生物标志物 DUSRC 内的研究项目。此外，ACC 还提供分析专业知识来识别 使用高分辨率质量分析这些超级基金污染物的转化产物和/或代谢物 光谱测定技术。在过去的资助期间，ACC 一直是 DUSRC 的重要组成部分， 包括开发有机和无机化合物的新分析方案以及分析 超过 2,000 个样品，用于各种分析物（例如阻燃剂、有机磷杀虫剂、 生物和环境样品中的 PAH 和微量金属）。高技术应用取得进展 分辨率质谱法和其他方法用于新型药物的非靶向和效果导向分析 环境和生物领域超级基金相关化学品的污染物和转化产物 媒体。在此，我们建议继续并扩大 ACC 的活动，重点是有针对性地分析 生物和环境样品中的多环芳烃、金属、神经递质生物标志物以及非靶向和 PAH 化合物的转化产物分析。此外，我们建议加强 ACC 的作用， 通过分析复杂的环境混合物（例如渗滤液）来协调跨项目合作 项目 1 和 5 将产生并共享给项目 2、3和4用于评估潜在毒性。 ACC 将参与研究经验和培训 核心是领导与分析化学相关的培训和咨询活动。 ACC团队还将提供 样品制备方法和仪器分析培训的非正式实践指导。我们 预计这些相互作用将有助于指导研究人员进行研究活动的实验设计 这将需要化学分析。最后，我们将利用我们的专业知识来开发分析方法和 对实验过程中可能出现的其他超级基金相关化合物进行分析 复杂的混合物并在超级基金站点进行研究。这些活动将借鉴我们迄今为止取得的巨大成功 开发、验证和应用环境和生物污染物分析方法 样品。