Neuropathology Core

神经病理学核心

基本信息

批准号：
10187488
负责人：
Andrew Franklin Teich
金额：
$ 30.84万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-06-15 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10187488
关键词：
Affect Aliquot Alzheimer&apos s Disease Alzheimer&apos s disease related dementia Anatomy Area Autopsy Bar Codes Biological Brain Cell Nucleus Cessation of life Clinical Communities Contralateral Diagnosis Diagnostic Diagnostic Services Disease Dissection Education Evaluation Faculty Formalin Freezing Fusiform gyrus Genes Goals Grouping Hippocampus (Brain)Human Individual Institutes Institution International Investigation Methodology Microscopic Mission Modeling Molecular Neurodegenerative Disorders Neurology Pathogenesis Pathologic Pathology Pathway interactions Patients Pattern Periodicity Procedures Process Protocols documentation Publications Recording of previous events Research Research Personnel Resources Sampling Services Site Slide Speed TREM2 gene Tissues Training Universities Validation Variant Visit Visual Cortex animal resource brain tissue cell type entorhinal cortex experience frontal lobe human tissue multidisciplinary neuropathology next generation novel operation ranpirnase symposium tau Proteins transcriptome sequencing

项目摘要

NEUROPATHOLOGY CORE PROJECT SUMMARY/ABSTRACT The importance of human brain tissue in ADRD research cannot be overstated, both as a resource to explore and define novel molecular pathways, as well as a critical resource for animal researchers who wish to validate their findings in human tissue. The AMP-AD initiative recently launched by NIA is further validation of the importance of human brain tissue in defining disease-relevant pathways, and has resulted in numerous publications that have added to our evolving understanding of AD pathogenesis. The Columbia University ADRC Neuropathology (NP) Core has a long history of serving the wider ADRD research community’s need for well characterized human brain tissue, and we will continue this mission under this new P30 application. The chief function of the NP Core is to provide state-of-the-art diagnostic services, and to collect, maintain, and distribute optimally prepared brain samples to researchers at Columbia and throughout the world. The NP Core also has a responsibility to train and educate the next generation of neurodegenerative disease researchers and brain bankers. Finally, the NP Core will contribute to the investigation of the three pathways that are the scientific focus of this application. With regards to tissue banking and distribution, the NP Core has a well-established procedure for receiving and banking brains, and we will continue this protocol for this new P30 application. Upon death of a donor, one half brain is immersed in formalin and kept for thorough neuropathological evaluation, and the contralateral half is extensively dissected at the fresh state and processed to yield up to 150 blocks and pulverized aliquots of parenchyma. Our samples are barcoded and electronically tracked, which aids in organizing the samples, maintaining them safely, and ultimately speeds the selection of samples for research. With regards to education, the NP Core has a long-standing tradition of educating the next generation of neuroscientists and brain bankers, through weekly brain cutting, monthly clinicopathological conferences, and periodic hosting of visiting neuroscientists throughout the world (since 2001, our methodology has been fully or partially instituted at eight other academic sites). Finally, we will use the resources of the NP core to inform on the three AD-associated biological pathways. Specifically, we will use single-nucleus RNA-seq to ask the following questions: 1) What cell types express the three established genes that relate to the three pathways (i.e. TREM2, APOE, SORL1)?; 2) Is there regional variation in the cell types that express these three genes?, and 3) Does this expression pattern change during successive Braak stages?

神经病理学核心项目摘要/摘要人类脑组织在 ADRD 研究中的重要性怎么强调都不为过，无论是作为探索的资源并定义新的分子途径，以及希望验证的动物研究人员的关键资源 NIA 最近发起的 AMP-AD 计划进一步验证了他们在人体组织中的发现。人类脑组织在定义疾病相关途径中的重要性，并导致了许多哥伦比亚大学 ADRC 的出版物增加了我们对 AD 发病机制不断发展的理解。神经病理学 (NP) 核心在满足更广泛的 ADRD 研究界的良好需求方面有着悠久的历史描述了人类脑组织的特征，我们将在新的 P30 应用程序下继续这一使命。 NP Core 的功能是提供最先进的诊断服务，并收集、维护和分发 NP Core 还为哥伦比亚大学和世界各地的研究人员提供了最佳制备的大脑样本。有责任培训和教育下一代神经退行性疾病研究人员和大脑最后，NP 核心将有助于研究科学焦点的三个途径。此应用程序的。关于组织库和分发，NP Core 拥有完善的接收和分发程序大脑库，我们将在捐赠者去世后继续这个新的 P30 应用程序，一半。将大脑浸入福尔马林中并保存以进行彻底的神经病理学评估，并将对侧一半通常在新鲜状态下进行解剖并加工以产生多达 150 块和粉碎等分试样我们的样品带有条形码并进行电子跟踪，这有助于组织样品，安全地维护它们，并最终加快研究样本的选择， NP 核心有着教育下一代神经科学家和脑银行家的悠久传统，通过每周一次的脑部切割、每月一次的临床病理学会议以及定期举办来访世界各地的神经科学家（自 2001 年以来，我们的方法已在 8 个国家或地区全部或部分采用）最后，我们将利用 NP 核心的资源来介绍与 AD 相关的三个内容。具体来说，我们将使用单核 RNA-seq 来提出以下问题：1）什么？细胞类型表达与三种途径相关的三种已确定基因（即 TREM2、APOE、SORL1）？； 2) 表达这三种基因的细胞类型是否存在区域差异？ 3) 这种表达是否存在差异？连续布拉克阶段的模式变化？