Neuropathology Core

神经病理学核心

基本信息

批准号：
10413097
负责人：
Andrew Franklin Teich
金额：
$ 31.84万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-06-15 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10413097
关键词：
Affect Aliquot Alzheimer&apos s Disease Alzheimer&apos s disease related dementia Anatomy Area Autopsy Bar Codes Biological Brain Cell Nucleus Cessation of life Clinical Communities Contralateral Diagnosis Diagnostic Diagnostic Services Disease Dissection Education Evaluation Faculty Formalin Freezing Fusiform gyrus Genes Goals Grouping Hippocampus (Brain)Human Individual Institutes Institution International Investigation Methodology Microscopic Mission Modeling Molecular Neurodegenerative Disorders Neurology Pathogenesis Pathologic Pathology Pathway interactions Patients Pattern Periodicity Procedures Process Protocols documentation Publications Recording of previous events Research Research Personnel Resources Sampling Services Site Slide Speed TREM2 gene Tissues Training Universities Validation Variant Visit Visual Cortex animal resource brain tissue cell type entorhinal cortex experience frontal lobe human tissue multidisciplinary neuropathology next generation novel operation ranpirnase symposium tau Proteins transcriptome sequencing

项目摘要

NEUROPATHOLOGY CORE PROJECT SUMMARY/ABSTRACT The importance of human brain tissue in ADRD research cannot be overstated, both as a resource to explore and define novel molecular pathways, as well as a critical resource for animal researchers who wish to validate their findings in human tissue. The AMP-AD initiative recently launched by NIA is further validation of the importance of human brain tissue in defining disease-relevant pathways, and has resulted in numerous publications that have added to our evolving understanding of AD pathogenesis. The Columbia University ADRC Neuropathology (NP) Core has a long history of serving the wider ADRD research community’s need for well characterized human brain tissue, and we will continue this mission under this new P30 application. The chief function of the NP Core is to provide state-of-the-art diagnostic services, and to collect, maintain, and distribute optimally prepared brain samples to researchers at Columbia and throughout the world. The NP Core also has a responsibility to train and educate the next generation of neurodegenerative disease researchers and brain bankers. Finally, the NP Core will contribute to the investigation of the three pathways that are the scientific focus of this application. With regards to tissue banking and distribution, the NP Core has a well-established procedure for receiving and banking brains, and we will continue this protocol for this new P30 application. Upon death of a donor, one half brain is immersed in formalin and kept for thorough neuropathological evaluation, and the contralateral half is extensively dissected at the fresh state and processed to yield up to 150 blocks and pulverized aliquots of parenchyma. Our samples are barcoded and electronically tracked, which aids in organizing the samples, maintaining them safely, and ultimately speeds the selection of samples for research. With regards to education, the NP Core has a long-standing tradition of educating the next generation of neuroscientists and brain bankers, through weekly brain cutting, monthly clinicopathological conferences, and periodic hosting of visiting neuroscientists throughout the world (since 2001, our methodology has been fully or partially instituted at eight other academic sites). Finally, we will use the resources of the NP core to inform on the three AD-associated biological pathways. Specifically, we will use single-nucleus RNA-seq to ask the following questions: 1) What cell types express the three established genes that relate to the three pathways (i.e. TREM2, APOE, SORL1)?; 2) Is there regional variation in the cell types that express these three genes?, and 3) Does this expression pattern change during successive Braak stages?

神经病理学核心项目摘要/摘要人脑组织在ADRD研究中的重要性不能被夸大，这既是探索的资源并为希望验证的动物研究人员定义新的分子途径以及关键资源他们在人体组织中的发现。 NIA最近发起的AMP-AD计划是进一步验证人脑组织在定义与疾病相关的途径中的重要性，并导致了许多增加了我们对AD发病机理不断发展的理解的出版物。哥伦比亚大学ADRC 神经病理学（NP）核心在为更广泛的ADRD研究社区的需求服务方面有悠久的历史人类脑组织的特征是，我们将在此新的P30应用下继续这项任务。酋长 NP核心的功能是提供最先进的诊断服务，并收集，维护和分发为哥伦比亚和世界各地的研究人员提供最佳准备的大脑样本。 NP核心也有培训和教育下一代神经退行性疾病研究人员和大脑的责任银行家。最后，NP核心将有助于三个途径的投资，这是科学重点此应用程序。关于组织银行和分配，NP核心具有公认的接收程序和银行大脑，我们将继续为此新的P30应用程序进行此协议。捐助者去世后一半大脑浸入福尔马林并保存以进行彻底的神经病理学评估，对侧的一半是在新鲜状态进行广泛解剖，并处理多达150个块，并粉碎的等分试样实质。我们的样品是条形码和电子跟踪的，有助于组织样品，安全地维护它们，并最终加快了研究样本的选择。关于教育， NP核心具有长期的传统，即教育下一代神经科学家和脑银行家，通过每周的大脑切割，每月的临床病理学会议和定期托管全世界的神经科学家（自2001年以来，我们的方法已经完全或部分建立了八点其他学术网站）。最后，我们将使用NP核心的资源来告知这三个广告相关的生物途径。具体来说，我们将使用单核RNA-Seq提出以下问题：1）细胞类型表达与三个途径相关的三个已建立基因（即TREM2，APOE，SORL1）？ 2）表达这三个基因的细胞类型中是否存在区域变化？成功的Braak阶段变化？