Determining the levels of resistance and patterns of cross-resistance for common kdr alleles in Aedes aegypti

确定埃及伊蚊常见 kdr 等位基因的耐药水平和交叉耐药模式

• 批准号: 9978470
• 负责人: Jeffrey G Scott
• 金额: $ 26.62万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-02-25 至 2022-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9978470
• 关键词: Adult; Aedes; Affect; Alleles; Culicidae; Data; Dengue; Evolution; Goals; Individual; Insecticide Resistance; Insecticides; Measures; Monitor; Mutation; Pattern; Phenotype; Population; Population Genetics; Process; Recommendation; Research; Resistance; Resistance development; Sodium Channel; ZIKA; congenic; design; disorder control; genetic resistance; human disease; improved; programs; pyrethroid; resistance allele; resistance mechanism; vector; vector control; vector mosquito; voltage

SUMMARY/ABSTRACT: Here we seek to understand the evolution of resistance to pyrethroid insecticides in Aedes aegypti, an important vector of human diseases such as dengue and Zika. Pyrethroid insecticides are widely used for control of adult Aedes, but resistance has evolved in many populations, representing global challenge for control of this vector and the diseases it transmits. Finding strategies for delaying the development of resistance by mosquito vectors to the few available insecticides is critical. To do so, it is essential to identify the level of resistance conferred, by common resistance alleles, to insecticides that target the voltage sensitive sodium channel (VSSC). This information will fill a critical data gap in our understanding of the evolution of resistance and will inform what options exist in populations where the different resistance alleles are found. Our long-term goals are to understand the importance of different Vssc alleles in the evolution of resistance so that this important process can be understood and the development of resistance can be delayed. Our short-term goal is to measure the level of resistance conferred by different Vssc mutations found in populations across the globe. To accomplish this goal we will pursue two specific aims. Aim 1. Isolate seven congenic (to the susceptible Liverpool strain) strains that have one of the common unstudied Vssc resistance alleles. Aim 2. Determine the level of resistance conferred by each Vssc allele to twenty insecticides that target the VSSC. This proposal fills an important data gap in our understanding of insecticide resistance in A. aegypti by quantitating the phenotype that the different alleles confer. In addition, our results will demonstrate what cross-resistance spectrum is conferred by each allele. This information will provide us with a vastly improved understanding of pyrethroid resistance in A. aegypti, and yield key information for resistance management in this important vector of human disease.

摘要/摘要： 在这里，我们试图了解埃及埃及拟南芥杀虫剂的耐药性的演变， 人类疾病的重要媒介，例如登革热和寨卡病毒。拟除虫菊酯杀虫剂广泛 用于控制成年艾德斯的人，但在许多人群中都在发展，代表全球 控制该向量及其传播的疾病的挑战。寻找延迟的策略 蚊子对少数可用杀虫剂的抗性发展至关重要。做 因此，必须确定通过公共电阻等位基因赋予的阻力水平 靶向电压敏感钠通道（VSSC）的杀虫剂。此信息将填充 关键的数据差距在我们对抵抗演变的理解中，并将告知哪些选择 存在于发现不同电阻等位基因的种群中。 我们的长期目标是了解不同VSSC等位基因在演变中的重要性 抵抗力，以便可以理解这一重要过程，并且抵抗的发展可以 延迟。 我们的短期目标是测量不同VSSC突变所赋予的阻力水平 在全球范围内发现。为了实现这一目标，我们将追求两个具体的目标。 目标1。分离七个先天性（易感利物浦菌株），具有其中之一 常见的未研究的VSSC电阻等位基因。 目标2。确定每个VSSC等位基因赋予二十种杀虫剂的电阻水平 该针对VSSC。 该提案填补了我们对A. a. egypti中杀虫剂耐药性的重要数据差距 通过量化不同等位基因赋予的表型。此外，我们的结果将 演示每个等位基因赋予了哪些交叉抗性光谱。这些信息将 为我们提供对A. eegypti中拟除虫菊酯耐药性的大大提高的理解，并产生 在人类疾病的重要媒介中，抵抗管理的关键信息。