Characterization of an extracelluar structure produced by Staphylococcus aureus

金黄色葡萄球菌产生的细胞外结构的表征

• 批准号: 8584254
• 负责人: BLAISE R BOLES
• 金额: $ 19.01万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-12-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8584254
• 关键词: Amino Acid Sequence; Amyloid fibers; Animal Model; Cell Wall; Cells; Communicable Diseases; Data; Development; Electrons; Environment; Exposure to; Extracellular Structure; Fiber; Growth; Health; Human; Individual; Infection; Integration Host Factors; Knowledge; Laboratories; Life; Microbial Biofilms; Molecular; Nose; Peptide Hydrolases; Peptides; Phenols; Population; Regulation; Resistance; Risk Factors; Rodent Model; Role; Staphylococcus aureus; Structure; Toxin; Translating; United States; Vision; base; beta pleated sheet; cost; extracellular; mutant; novel; pathogen; prevent; public health relevance; surfactant

DESCRIPTION (provided by applicant): Staphylococcus aureus is a medically important human pathogen that is found in the nasal passages of approximately 1/3 of the human population. The nose serves as a reservoir for spread of this pathogen and predisposes the host to potential infection. Despite the importance of S. aureus to human health, molecular mechanisms and host factors influencing nasal colonization are not well understood. We have recently discovered an extracellular amyloid fiber produced by S. aureus during nasal colonization. This finding is extremely novel and exciting as S. aureus has never been shown to produce extracellular structures. We hypothesize that this extracellular fiber is important for S. aureus nasal colonization and persistence within the nose. Our preliminary results suggest that the fibers contribute to biofilm development and consist of multiple toxins, which are folding into beta sheet rich confirmations to self-assemble into amyloid fibers. The aims of this proposal will determine the composition, formation, degradation and role of S. aureus fibers during nasal colonization and biofilm development. At the conclusion of these studies, we will have expanded our knowledge of S. aureus nasal colonization and characterized a novel extracellular structure produced by this important commensal and pathogen.

描述（由申请人提供）：金黄色葡萄球菌是一种医学上重要的人类病原体，在大约 1/3 人口的鼻道中发现。鼻子是这种病原体传播的储存库，使宿主容易受到潜在的感染。尽管金黄色葡萄球菌对人类健康很重要，但影响鼻定植的分子机制和宿主因素尚不清楚。我们最近发现了金黄色葡萄球菌在鼻定植过程中产生的细胞外淀粉样纤维。这一发现非常新颖和令人兴奋，因为金黄色葡萄球菌从未被证明能够产生细胞外结构。我们假设这种细胞外纤维对于金黄色葡萄球菌鼻腔定植和在鼻子内的持续存在很重要。我们的初步结果表明，这些纤维有助于生物膜的形成，并由多种毒素组成，这些毒素会折叠成 富含β片层的确认可以自组装成淀粉样纤维。该提案的目的将确定金黄色葡萄球菌纤维在鼻定植和生物膜发育过程中的组成、形成、降解和作用。在这些研究结束时，我们将扩展我们对金黄色葡萄球菌鼻定植的了解，并表征这种重要的共生菌和病原体产生的新型细胞外结构。