Augmenting Vocal Fold Repair Using an Optimized Lamina Propria Extracellular Matrix Derived Hydrogel

使用优化的固有层细胞外基质衍生水凝胶增强声带修复

基本信息

批准号：
9926119
负责人：
Donald O Freytes
金额：
$ 57.4万
依托单位：
NORTH CAROLINA STATE UNIVERSITY RALEIGH
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-05-04 至 2024-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9926119
关键词：
Address Affect American Anatomy Architecture Area Biocompatible Materials Biomechanics Bladder Cells Cicatrix Clinical Communication impairment Data Derivation procedure Detergents Disease Dysphonia Engineering Etiology Extracellular Matrix Fibroblasts Fibrosis Foundations Gel Goals Human Hydrogels Impairment In Situ In Vitro Injectable Injury Investigation Laboratories Lamina Propria Link Location Mechanics Methods Modeling Mucous Membrane National Institute on Deafness and Other Communication Disorders Oryctolagus cuniculus Outcome Patient Care Patient-Focused Outcomes Patients Performance Physiology Pliability Pre-Clinical Model Procedures Process Production Property Protocols documentation Public Health Reflux Reporting Research Role Small Intestinal Submucosa Stimulus Stomach Strategic Planning Structure Symptoms Testing Therapeutic Time Tissues Trauma Treatment Cost Voice Voice Disorders Wages Work biomaterial compatibility biomaterial development clinical application clinical practice cohort experimental study healing iatrogenic injury improved in vivo macrophage minimally invasive preclinical trial repaired response scaffold tissue repair vocal cord wound healing

项目摘要

PROJECT SUMMARY Voice impairment is the most common communication disorder with nearly 20 million people in the US reporting symptoms of dysphonia annually. The cost of treatment and lost wages for this disorder is approaching $13 billion. The etiology of these disorders is diverse, but given their anatomic location, the vocal folds (VFs) are susceptible to a multitude of injurious stimuli, including reflux of gastric contents, iatrogenic injury, and the inherent trauma associated with voice production. Injury can result in altered lamina propria (LP) architecture resulting in aberrant phonatory physiology. To date, no treatment restores the native VF extracellular matrix (ECM) composition, structure, and function following VF injury which likely underlies suboptimal outcomes for patients with VF scar. Extracellular matrix derived scaffolds, if appropriately processed, can be used as resorbable and naturally derived biomaterials with a safe record of clinical use that promote constructive tissue remodeling. Tissue- specific ECM hydrogels are ideal for clinical application, particularly given the emerging practice and distinct advantages of in-office procedures using minimally invasive approaches. Work from our group and others provide promising data regarding the role of decellularized vocal fold lamina propria (VFLP-ECM) as a therapeutic scaffold. We broadly hypothesize that VFLP-ECM will evolve into clinical practice to provide a scaffold that promotes functional repair of the VFs. Specifically, we seek to develop an injectable vocal fold lamina propria hydrogel (VFLP-ECMh) that will degrade over time while harnessing the stimulatory effects of the ECM to drive vocal fold tissue remodeling.

项目摘要语音障碍是最常见的通信障碍，在美国近2000万人报告每年吞咽困难的症状。这种疾病的治疗成本和损失的工资接近13美元十亿。这些疾病的病因是多种多样的，但是鉴于它们的解剖位置，声带褶皱（VFS）是容易受到多种伤害刺激的影响，包括胃含量的反流，医源性损伤和与语音产生相关的固有创伤。受伤会导致固有椎板（LP）结构改变导致异常语音生理。迄今为止，尚未恢复本地VF细胞外基质（ECM）VF损伤后的组成，结构和功能，这可能是次优的结果 VF疤痕患者。细胞外基质衍生的支架（如果经过适当处理）可以用作吸收和自然衍生的生物材料具有安全的临床用途记录，可促进建设性组织重塑。组织- 特定的ECM水凝胶非常适合临床应用，特别是考虑到新兴实践和不同使用最低侵入性方法的办公室程序的优势。我们小组和其他人的工作提供有关脱细胞声折叠层（VFLP-ECM）作用的有希望的数据治疗脚手架。我们广泛地假设VFLP-ECM将演变为临床实践，以提供促进VF的功能修复的脚手架。具体来说，我们试图开发一个可注射的声音固定水凝胶（VFLP-ECMH）会随着时间的流逝而降解，同时利用刺激作用 ECM驱动声带组织重塑。