Optimal Tissue Engineering Approach for Treating Injured Vocal Folds

治疗受损声带的最佳组织工程方法

基本信息

批准号：
10058832
负责人：
Donald O Freytes
金额：
$ 59.53万
依托单位：
NORTH CAROLINA STATE UNIVERSITY RALEIGH
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-12-01 至 2023-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10058832
关键词：
Address American Anatomy Architecture Area Autologous Biocompatible Materials Biomechanics Bladder Cell Therapy Cells Cellular Structures Cicatrix Clinical Communication impairment Data Disease Dysphonia Engineering Etiology Extracellular Matrix Fibroblasts Fibrosis Foundations Gel Human Hydrogels Impairment In Vitro Inflammation Inflammatory Injectable Injury Investigation Laboratories Lamina Propria Location Mechanics Mesenchymal Stem Cells Methods Modality Mucous Membrane National Institute on Deafness and Other Communication Disorders Organ Outcome Outpatients Particulate Patient-Focused Outcomes Patients Physiology Pliability Pre-Clinical Model Procedures Process Production Public Health Reflux Regenerative response Reporting Role Site Small Intestinal Submucosa Source Stimulus Stomach Strategic Planning Structure Symptoms Therapeutic Therapeutic Effect Time Tissue Engineering Tissues Trauma Treatment Cost Voice Voice Disorders Wages Work biomaterial development clinical application clinical material clinical practice clinical trial implementation experimental study functional restoration healing iatrogenic injury implantation improved in vitro activity in vivo individual variation injured insight macrophage minimally invasive novel regenerative repaired response response to injury scaffold stem cell therapy stem cells vocal cord wound healing

项目摘要

PROJECT SUMMARY Voice impairment is the most common communication disorder with nearly 20 million people in the US reporting symptoms of dysphonia annually. The cost of treatment and lost wages for this disorder is approaching $13 billion. The etiology of these disorders is diverse, but given their anatomic location, the vocal folds (VFs) are susceptible to a multitude of injurious stimuli, including reflux of gastric contents, iatrogenic injury, and the inherent trauma associated with voice production. Injury can result in altered lamina propria (LP) architecture resulting in aberrant phonatory physiology. To date, no treatment restores the native VF extracellular matrix (ECM) composition, structure, and function following VF injury which likely underlies suboptimal outcomes for patients with VF scar. ECMs, if appropriately processed, can be used as resorbable, clinically safe, and naturally derived biomaterials that promote tissue remodeling while reducing fibrosis. Injectable ECM hydrogels are ideal for clinical application and cell delivery, particularly given the emerging practice and distinct advantages of in-office procedures using minimally invasive approaches. Work from our group and others provide promising data regarding the role of decellularized vocal fold lamina propria (VFLP-ECM) as an injectable agent or as a delivery vehicle for therapeutic cells such as mesenchymal stem cells. We broadly hypothesize that VFLP-ECM hydrogels (with or without stem cells) will promote functional repair of the VFs.

项目摘要语音障碍是最常见的通信障碍，在美国近2000万人报告每年吞咽困难的症状。这种疾病的治疗成本和损失的工资接近13美元十亿。这些疾病的病因是多种多样的，但是鉴于它们的解剖位置，声带褶皱（VFS）是容易受到多种伤害刺激的影响，包括胃含量的反流，医源性损伤和与语音产生相关的固有创伤。受伤会导致固有椎板（LP）结构改变导致异常语音生理。迄今为止，尚未恢复本地VF细胞外基质（ECM）VF损伤后的组成，结构和功能，这可能是次优的结果 VF疤痕患者。如果经过适当处理，ECM可以用作吸收，临床安全和自然衍生的生物材料促进组织重塑的同时减少纤维化。可注射的ECM水凝胶是临床应用的理想选择和细胞传递，尤其是考虑到新兴实践和使用的明显优势微创方法。我们小组和其他人的工作提供了有关角色的有希望的数据作为注射剂或作为输送工具治疗细胞，例如间充质干细胞。我们广泛地假设VFLP-ECM水凝胶（有或没有干细胞）将促进VF的功能修复。