MINORITY PREDOCTORAL FELLOWSHIP PROGRAM

少数族裔博士前奖学金计划

• 批准号: 7151597
• 负责人: Donald O Freytes
• 金额: $ 4.35万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7151597
• 关键词: bone marrow; digestion; model; peptides; polymers; proteins; tissues; urea; urinary bladder

DESCRIPTION (provided by applicant): Extracellular matrix (ECM) scaffolds have been successfully used in more than 300,000 human patients in both soft and hard tissue replacement applications. ECM scaffolds promote site specific tissue remodeling and are completely and rapidly degraded during the remodelling process. However, ECM-derived scaffolds are limited by the inherent mechanical and material properties of the tissue from which they are derived. Electrospun poly(ester-urethane)urea (PEUU) scaffolds represent an alternative elastomeric material that recreates the fibrous ultrastructure of the ECM. However, PEUU scaffolds lack the bioactivity inherent in the ECM scaffolds. It is the central hypothesis of the proposed research that a hybrid scaffold composed of solubilized ECM and PEUU into electrospun material will result in a bioactive elastomeric scaffold with predictable physical properties. Three specific aims are proposed: (1) to solubilize a porcine urinary bladder ECM via enzymatic degradation; (2) to electrospin and characterize a combination of the urinary bladder ECM with PEUU; and (3) to characterize the host tissue response to both the digested ECM and the polymer-ECM scaffold after implantation in an in vivo model.

描述（由申请人提供）：细胞外基质 (ECM) 支架已成功用于超过 300,000 名人类患者的软组织和硬组织替代应用。 ECM支架促进位点特异性组织重塑，并在重塑过程中完全快速降解。然而，ECM 衍生的支架受到其来源组织的固有机械和材料特性的限制。电纺聚（酯-氨基甲酸酯）脲（PEUU）支架代表了一种替代弹性体材料，可以重建 ECM 的纤维超微结构。然而，PEUU 支架缺乏 ECM 支架固有的生物活性。该研究的中心假设是，由溶解的 ECM 和 PEUU 组成的电纺材料混合支架将产生具有可预测物理特性的生物活性弹性体支架。提出了三个具体目标：（1）通过酶降解溶解猪膀胱 ECM； (2) 静电纺丝并表征膀胱 ECM 与 PEUU 的组合； (3) 表征植入体内模型后宿主组织对消化的 ECM 和聚合物-ECM 支架的反应。