Dynamics of targeted gene knockdowns in A. burtoni

伯托尼靶向基因敲低的动态

• 批准号: 8701415
• 负责人: RUSSELL D FERNALD
• 金额: $ 7.85万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-08-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8701415
• 关键词: Adult; Africa; African; Animals; Antisense Oligonucleotides; Behavior; Behavior Control; Behavioral; Behavioral Model; Biological Models; Candidate Disease Gene; Cichlids; Code; Complex; Cues; Embryo; Environment; Functional RNA; Gene Expression; Gene Expression Profile; Gene Silencing; Gene Targeting; Genes; Genome; Genomics; Goals; Human; Maps; Measures; Mental Depression; Mental disorders; Methods; MicroRNAs; Modeling; Molecular; Neuronal Plasticity; Neurons; Outcome; Pattern; Penetrance; Phenotype; Physiological; Pilot Projects; Role; Schizophrenia; Social Behavior; Social status; System; Techniques; Testing; Tissues; Toxic effect; Transcript; Transgenic Animals; Transgenic Organisms; animal data; genome sequencing; in vivo; novel; public health relevance; research study; response; social; tool; transcriptomics

DESCRIPTION (provided by applicant): This project will develop gene knockdown methods for a novel behavioral model system. Animals we study respond to social cues behaviorally and physiologically, changing phenotype as they ascend or descend in social status. Specifically, we can manipulate the social system to mimic the natural environment and have identified neurons that respond to social information. We have collected gene expression data from animals of distinct, socially regulated phenotypes producing candidate genes that putatively regulate social behavior. We will develop methods to silence gene expression to test which genes are responsible for particular aspects of behavioral change.

描述（由申请人提供）：该项目将为新型行为模型系统开发基因敲除方法。我们研究的动物在行为和生理上对社会线索做出反应，随着社会地位的上升或下降而改变表型。具体来说，我们可以操纵社会系统来模仿自然环境，并识别出对社会信息做出反应的神经元。我们从具有独特的社会调节表型的动物中收集了基因表达数据，产生了推测调节社会行为的候选基因。我们将开发沉默基因表达的方法，以测试哪些基因负责行为变化的特定方面。