Castles made of sand: The genomics of complex behavior

沙子城堡：复杂行为的基因组学

基本信息

批准号：
8631179
负责人：
RUSSELL D FERNALD
金额：
$ 30.18万
依托单位：
GEORGIA INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-05-01 至 2018-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8631179
关键词：
Address Affect Africa Alleles Animal Model Animals Behavior Behavior monitoring Behavioral Behavioral Genetics Biological Models Brain Brain Diseases Brain Part Brain region Cell Nucleus Cichlids Complex Courtship Data Deposition Development Diagnosis Disease Elements Environment Event Female Fishes Gene Expression Gene Expression Profile Genes Genetic Genome Genomics Goals Health Human Hybrids Immediate-Early Genes Inbreeding Individual Knowledge Laboratories Malawi Maps Measures Modeling Mutation National Human Genome Research Institute Natural Selections Nature Neural Pathways Neurons Partner in relationship Phase Phenotype Plant Roots Problem Solving Quantitative Trait Loci Recruitment Activity Regulation Reproduction Research Research Project Grants Silicon Dioxide Social Behavior Spottings Standardization System Testing Time Transcript Variant Vertebrates base design genetic analysis genome sequencing male nervous system disorder neural circuit novel offspring pressure public health relevance relating to nervous system reproductive social transcriptome sequencing

项目摘要

Summary/Abstract The goal of the proposed collaborative research is to understand how distinct social behaviors are encoded in the genome and wired in the brain. Using a novel natural vertebrate system we will trace key behaviors to their genomic roots. Specifically, we will study innate 'bower' (reproductive nest) construction by male cichlid fishes from Lake Malawi, East Africa. This behavior is an essential part of social and reproductive interactions between males and females. In Aim 1, we will quantify the elements of bower behavior, comparing pit-digging vs. castle-building cichlid species and their F1 hybrids. Aim 2 will map brain regions recruited during male bower behavior by visualizing the expression of "immediate early" genes that reflect neural activity. Transcriptional divergence in these focal brain regions will then be quantified by allele-specific expression analysis in F1 hybrid males, who construct bowers in a phased sequence of species-specific behaviors. Aim 3 will identify regions of the genome responsible for differences in bower behavior by re-sequencing the genomes of bulked F2 individuals who either dig pits or build castles. Our integrative approach is designed to solve problems that are currently impossible, or at best difficult to study in standard inbred model organisms. Our strategy to identify the genetic control of neural circuits regulating essential behaviors in outbred vertebrates will provide a natural model of human behavioral diversity.

摘要/摘要拟议的合作研究的目标是了解不同的社会行为如何被编码在基因组中并连接到大脑中。使用一种新颖的自然脊椎动物系统，我们将追踪关键行为的基因组根源。具体来说，我们将研究先天的“凉亭” （繁殖巢）由来自东非马拉维湖的雄性丽鱼建造。这行为是男性和女性之间社会和生殖互动的重要组成部分女性。在目标 1 中，我们将量化凉亭行为的要素，比较挖坑与挖坑。城堡建造丽鱼科鱼种及其 F1 杂交种。目标 2 将绘制招募的大脑区域图在雄性凉亭行为期间，通过可视化“立即早期”基因的表达，反映神经活动。这些大脑焦点区域的转录分歧将是通过 F1 杂交雄性的等位基因特异性表达分析进行量化，这些雄性在物种特定行为的阶段序列。目标 3 将识别基因组区域通过对大量 F2 的基因组进行重新测序，导致凉亭行为的差异挖坑或建造城堡的人。我们的综合方法旨在解决目前在标准近交模型中不可能或最多难以研究的问题有机体。我们的策略是确定神经回路调节的遗传控制近交脊椎动物的行为将提供人类行为多样性的自然模型。