Novel optical probe for dopamine release in neural circuits

用于神经回路中多巴胺释放的新型光学探针

• 批准号: 9766299
• 负责人: Marla Feller
• 金额: $ 21.36万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9766299
• 关键词: Acute; Address; Adult; Amacrine Cells; Axon; Behavior; Biological Models; Biomimetics; Brain; Calcium; Cells; Chemicals; Collaborations; Corpus striatum structure; Custom; Development; Dopamine; Electrical Synapse; Engineering; Exposure to; Fluorescence; Functional disorder; Goals; Image; In Vitro; Interneurons; Knockout Mice; Light; Light Adaptations; Lighting; Measures; Mediating; Microscope; Modernization; Monitor; Mus; Nervous system structure; Neuromodulator; Neurosciences; Optics; Pathologic; Pharmacology; Phase; Photoreceptors; Photosensitivity; Physiological; Play; Polymers; Positioning Attribute; Research; Retina; Retinal; Retinal Ganglion Cells; Rewards; Role; Scanning; Signal Transduction; Synapses; Techniques; Technology; Testing; Time; Tissues; Whole-Cell Recordings; Work; base; design; imaging modality; in vivo; innovative technologies; melanopsin; millisecond; motivated behavior; motor control; nanosensors; nervous system disorder; neural circuit; neuroregulation; neurotransmission; novel; professor; relating to nervous system; response; reuptake; sensor; single walled carbon nanotube; spatiotemporal; symposium; two photon microscopy; two-photon

A major focus of modern neuroscience is understanding the role of neuromodulators in neural circuit function and development, in behavior, and their dysfunction in neurological disease. Neural circuit research has been revolutionized by powerful new optical techniques. Here we propose to use new near-infrared optical nanosensor technology to image dopamine. As a proof of principle, we will use this sensor to monitor dopamine release under physiological conditions in both the developing and adult retinas. The ability to combine neuromodulator imaging with other physiological measures in a well-defined neural circuit will greatly advance our understanding of neuromodulation, allowing us to elucidate its many impacts on normal and pathological circuits.

现代神经科学的主要重点是了解神经调节剂的作用 在神经电路功能和发展中，行为及其功能障碍 神经疾病。神经电路研究已被强大的新革命 光学技术。在这里，我们建议使用新的近红外光学纳米传感器 图像多巴胺的技术。作为原则的证明，我们将使用此传感器 在生理条件下监测多巴胺在发育和发展中的释放 成人视网膜。将神经调节剂成像与其他生理学结合的能力 定义明确的神经电路中的措施将大大提高我们对 神经调节，使我们能够阐明其对正常和病理的许多影响 电路。