Cancer Proteomics Facility

癌症蛋白质组学设施

基本信息

批准号：
9753959
负责人：
NATHAN Andrew YATES
金额：
$ 7.46万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9753959
关键词：
Animals Antibodies Basic Science Bioinformatics Biological Biological Assay Biology Cancer Center Cancer Center Support Grant Cells Clinical Clinical Research Complex Data Detection Development Disease Doctor of Philosophy Grant Human Informatics Laboratories Leadership Liquid substance Malignant Neoplasms Mass Spectrum Analysis Measurement Measures Mission Modernization Molecular Patients Performance Physiology Pre-Clinical Model Preventive Intervention Protein Analysis Proteins Proteomics Research Research Activity Research Personnel Resource Sharing Sampling Sensitivity and Specificity Services Specificity Specimen Techniques Testing Time Tissues Training Translational Research Universities University of Pittsburgh Cancer Institute base cancer proteomics cloud based data management design experimental study improved instrumentation mass spectrometry shared resource medical schools metabolomics novel therapeutic intervention programs protein profiling tool translational study tumor

项目摘要

Abstract: Cancer Proteomics Facility (CPF) The Cancer Proteomics Facility (CPF) provides UPCI investigators access to state-of-the-art techniques and expertise for the detection, quantification, and characterization of cancer-related and biologically relevant proteins for basic, translational, and clinical studies. The Specific Aims of the CPF are to 1) provide expert guidance in the design and implementation of experiments that use modern protein analysis techniques, including statistical and bioinformatics analysis of the proteomic data; 2) collect, store, and optimally analyze proteins in a wide variety of samples including: cells, tissue, and clinically accessible fluids; 3) provide comprehensive protein identification and characterization services to UPCI investigators; 4) provide targeted quantitative protein assays to UPCI investigators; 5) perform unbiased protein profiling analyses (e.g. SILAC, ITRAQ, and Differential MS) of complex biological samples to identify cancer-related proteins; 6) provide training related to use of and access to all instrumentation and techniques used within CPF, and 7) enable investigators, through the development of a cloud based informatics platform, to use state-of-the-art data management, processing, and analysis tools for proteomic and metabolomic studies. During the past grant cycle, the UPCI and University of Pittsburgh School of Medicine mass spectrometry shared resource facilities have merged and new leadership has been hired to direct this service. Mass spectrometry-based proteomics techniques are now supported through a campus-wide Biomedical Mass Spectrometry Center that is housed on the Oakland campus and directed by Nathan Yates, PhD. This centralized shared resource provides UPCI investigators access to a wide array of high performance mass spectrometry techniques. Antibody based assays are supported by the Luminex laboratory in the Hillman Cancer Center that is directed by Anna Lokshin, PhD and specializes in commercial multiplexed bead based platforms. The CPF has assembled a broad and rapidly advancing set of techniques that allow each researcher to study cancer-related proteins with unmatched sensitivity and specificity. Together, the components of the CPF provide UPCI investigators access to new and specialized techniques for characterizing proteins in cell based animal and patient samples. As a new and growing component of many UPCI research initiatives, the CPF enables investigators to apply mass spectrometry-based, as well as Luminex bead-based, protein measurement techniques to the study of human cells, preclinical models, tumors, and other clinically accessible samples. During the current project period investigators in 9 UPCI Research Programs used the CPF.

摘要：癌症蛋白质组学设施（CPF）癌症蛋白质组学设施 (CPF) 为 UPCI 研究人员提供最先进的技术和癌症相关和生物学相关的检测、定量和表征的专业知识用于基础、转化和临床研究的蛋白质。 CPF 的具体目标是 1) 提供专家指导使用现代蛋白质分析技术的实验的设计和实施，包括蛋白质组数据的统计和生物信息学分析； 2）收集、存储和优化分析多种样品中的蛋白质，包括：细胞、组织和临床可用的液体； 3）提供为 UPCI 研究人员提供全面的蛋白质鉴定和表征服务； 4）提供有针对性的为 UPCI 研究人员提供定量蛋白质检测； 5) 进行无偏见的蛋白质分析（例如SILAC， ITRAQ 和微分 MS）分析复杂的生物样品，以识别癌症相关蛋白质； 6）提供与使用和获取 CPF 内使用的所有仪器和技术相关的培训，以及 7) 研究人员通过开发基于云的信息学平台，使用最先进的数据用于蛋白质组学和代谢组学研究的管理、处理和分析工具。在过去的补助期间周期，UPCI 和匹兹堡大学医学院质谱共享资源设施已合并，并聘请了新的领导层来指导这项服务。基于质谱的蛋白质组学现在，该技术由全校园生物医学质谱中心提供支持，该中心位于在奥克兰校区，由 Nathan Yates 博士执导。这种集中式共享资源提供了 UPCI 研究人员可以使用各种高性能质谱技术。基于抗体检测由 Anna 领导的 Hillman 癌症中心 Luminex 实验室提供支持 Lokshin 博士，专门研究基于商业多重珠的平台。 CPF 已组建了一个广泛且快速发展的技术集使每个研究人员能够研究与癌症相关的蛋白质无与伦比的灵敏度和特异性。 CPF 的各个组件共同为 UPCI 调查人员提供访问权限用于表征基于细胞的动物和患者样本中的蛋白质的新的专业技术。作为一个作为许多 UPCI 研究计划的新组成部分，CPF 使研究人员能够应用质量基于光谱法以及基于 Luminex 珠的蛋白质测量技术用于人类研究细胞、临床前模型、肿瘤和其他临床可获取的样本。在当前项目期间 9 个 UPCI 研究项目的研究人员使用了 CPF。