PROTEOMICS

蛋白质组学

基本信息

批准号：
8627424
负责人：
NATHAN Andrew YATES
金额：
$ 21.15万
依托单位：
SCRIPPS FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8627424
关键词：
Affect Age Aging Algorithms Antibodies Antioxidants Archives Automobile Driving Biochemical Bioinformatics Biological Biological Assay Biological Markers Biology Cell Aging Cells Complement Complex Computer software Conditioned Culture Media Data Data Set Degenerative Disorder Goals Health Human In Vitro Individual Instruction Intervention Label Longevity Mass Spectrum Analysis Measurement Measures Methodology Methods Mitochondria Molecular Mus Muscle Mutagens NF-kappa B Organism Oxidants Pathology Peptides Phenotype Plasma Proteins Proteomics Qualifying Quality Control Relative (related person)Research Personnel Role Sampling Serum Services Specificity Stem cells Techniques Therapeutic Tissues Training adult stem cell age related base design experience improved in vivo Model inhibitor/antagonist innovation insight instrumentation muscle aging novel p65 programs research study routine practice senescence stem stem cell therapy tissue regeneration tool young adult

项目摘要

PROJECT SUMMARY (See instructions): The Proteomics Core (Core D) will be responsible for providing the investigators of this Program Project with the proteomics data necessary to achieve their experimental aims. Unique methods (differential mass spectrometry) and extensive experience with proteomics on complex biologic materials will be utilized to discover circulating biomarkers of old age and proteins released from cells that promote cell senescence or tissue regeneration. By coordinating proteomic analyses within this Core, it will be possible to standardize methods and quality control, which will dramatically improve the sensitivity ofthe proteomics approaches. Comparisons between data sets produced for individual projects will significantly increase the value of each data set. For example, are factors secreted by young stem cells, which promote tissue regeneration (Project 3), also detected in the serum of Ercc1-/delta mice chronically treated with an inhibitor of NF-kB (Project 2) or a potent mitochondrial-targeted radical scavenger (Project 1), both of which promote health span and longevity? Comparisons between data sets will also greatly facilitate establishing priorities for which proteins/peptides to pursue. For example, a pro-longevity/anti-senescence factor is predicted to be secreted from young stem cells but not old stem cells or senesent cells/tissues. The same factor might also be induced by pro-longevity interventions (anti-oxidants, NF-kB inhibitors, stem cell therapy) but should not be induced by damaging agents (oxidants, genotoxins, old stem cells) allowing biology to dictate relative importance. In contrast, a single proteomics analysis for one project is comparatively extremely difficult to interpret because it yields a list of proteins/peptides and their relative abundance, which is not necessarily a determinant of biological importance. Hence this proteomics core as part of this Program Project is optimized to overcome several barriers impeding progress in the field and to have the greatest likelihood of identifying secreted and/or circulating factors that affect health span and lifespan. This will yield novel information about mechanisms of aging at the molecular level

项目摘要（参见说明）：蛋白质组学核心（核心 D）将负责为本计划项目的研究人员提供实现其实验目标所需的蛋白质组学数据。将利用独特的方法（差示质谱法）和复杂生物材料蛋白质组学的丰富经验来发现衰老的循环生物标志物和细胞释放的促进细胞衰老或组织再生的蛋白质。通过协调该核心内的蛋白质组学分析，将有可能标准化方法和质量控制，这将显着提高蛋白质组学方法的灵敏度。为各个项目生成的数据集之间的比较将显着增加每个数据集的价值。例如，年轻干细胞分泌的促进组织再生的因子（项目 3）也在长期接受 NF-kB 抑制剂（项目 2）或强效线粒体靶向药物治疗的 Ercc1-/delta 小鼠的血清中检测到。自由基清除剂（项目 1），两者都可以促进健康寿命和长寿？数据集之间的比较也将极大地促进确定蛋白质/肽的优先顺序。例如，预计年轻干细胞会分泌长寿/抗衰老因子，但老年干细胞或衰老细胞/组织不会分泌。延长寿命的干预措施（抗氧化剂、NF-kB 抑制剂、干细胞疗法）也可能会诱导相同的因素，但不应由破坏性物质（氧化剂、基因毒素、衰老干细胞）诱导，从而使生物学决定相对重要性。相比之下，一个项目的单一蛋白质组学分析相对来说极其难以解释，因为它会产生一系列蛋白质/肽及其相对丰度，而这不一定是生物学重要性的决定因素。因此，作为该计划项目一部分的蛋白质组学核心经过优化，以克服阻碍该领域进展的几个障碍，并最大可能地识别影响健康寿命和寿命的分泌和/或循环因素。这将在分子水平上产生有关衰老机制的新信息