Request for triple quadrupole mass spectrometer for the University of Pittsburgh

为匹兹堡大学申请三重四极杆质谱仪

• 批准号: 8640297
• 负责人: NATHAN Andrew YATES
• 金额: $ 48.73万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-01 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8640297
• 关键词: 5 year old; Award; Biochemical Pathway; Biological Assay; Biology; Blood capillaries; Complement; Contracts; Data; Development; Energy Metabolism; Funding; Grant; High Pressure Liquid Chromatography; Housing; Human; Ions; Mass Spectrum Analysis; Measurement; Measures; Metabolic; Metabolic Diseases; Mitochondria; Neonatal Screening; Pediatric Hospitals; Phenylalanine Metabolism Pathway; Productivity; Proteomics; Request for Proposals; Research; Research Personnel; Resort; Sampling; Services; System; Testing; Time; Toxic effect; United States National Institutes of Health; Universities; University of Pittsburgh Cancer Institute; Water; assay development; base; capillary; fatty acid oxidation; improved; instrument; instrumentation; mass spectrometer; meetings; member; metabolomics; nano; protein metabolite; quantum; research study; tool; ultra high pressure

DESCRIPTION (provided by applicant): This ORIP shared instrumentation proposal requests funding to purchase an Applied Biosystems SCIEX QTRAP 6500(R) triple quadruple / linear ion trap mass spectrometer, a WATERS Acquity(R) ultra high pressure liquid chromatography system, and the BIOCRATES MetIDQ" metabolite analysis kit. This instrument will be housed in the Biomedical Mass Spectrometry (BioMS) Center at the University of Pittsburgh; a newly created campus-wide center for proteomics and metabolomics. This integrated LC-MS/MS system will be used almost exclusively for the quantification of metabolites from five compound classes that have been identified as members of key biochemical pathways. Furthermore, the unique ion trapping capabilities of the QTRAP trap will be used to support elementary metabolite identification experiments and new assay development. Overall, the acquisition of this instrument will greatly enhance 7 NIH funded projects by enabling nine investigators to optimize well-controlled in-house analyses on valuable samples that are being studied as part of a focused effort to enhance the current understanding of energy metabolism disorders, improve newborn screening, and measure metabolic . Triple quadrupole LC-MS/MS is an essential service for the center, and one five year-old Thermo- Fisher Quantum Ultra is the only instrument of this type in the facility. This sole instrument is configured for proteomic analyses that employ nano-flow separations and tiny capillary columns. As configured, this instrument is not suitable for metabolite assays that use micro-flow separations and narrow bore columns. Furthermore, it is not practical to operate this one system as a "dual-purpose" instrument that is switched between protein and metabolite analysis without incurring a loss in productivity due to the significant switchover downtime. For these reasons, it has been difficult to meet the current and growing needed for metabolite quantification. Multiple NIH funded investigators have resorted to sending samples out to a patchwork of contract labs that provide limited assay capabilities and variable results. Therefore, the reasons for this application are to: 1) Provide a critically needed in-house metabolite quantitation capability; 2) Enable the development and testing of customized metabolite assays that are not easily obtained from contract labs. Relevance The overall impact of this new instrumentation to human is significant, as 9 independent researchers at the University of Pittsburgh (UP) including members of Children's Hospital (CH), and the University of Pittsburgh Cancer Institute (UPCI) require in-house research tools that are needed to improve and advance NIH funded research into the basic biology of energy metabolism, fatty acid oxidation, mitochondrial metabolism, and phenylalanine toxicity. LC-MS/MS based metabolic profiling is not currently available at the University of Pittsburgh. The acquisition of an AB SCIEX QTRAP 6500(R) triple quadruple / linear ion trap mass spectrometer would, for the first time, give these investigators and trainees access to this type of instrumentation, and allow a full complement of metabolite profile data to be under well controlled conditions. PUBLIC RELEVANCE: This ORIP shared instrumentation proposal requests funding to purchase an Applied Biosystems SCIEX QTRAP 6500(r) triple quadruple / linear ion trap mass spectrometer, a Waters Acquity(r) ultra high pressure liquid chromatography system, and the BIOCRATES MetIDQ(tm) metabolite analysis package. This instrument will be housed in the Biomedical Mass Spectrometry Center at the University of Pittsburgh; a newly created campus-wide center for proteomics and metabolomics. This integrated LC-MS/MS system will be used almost exclusively for the quantification of metabolites from five compound classes and provide essential data for 12 NIH funded grants awarded to 9 investigators who have significant and growing needs for mass spectrometry-based metabolite measurements. The primary reasons for this application are to: 1) Provide a critically needed in-house metabolite quantitation capability; 2) Enable the development and testing of customized metabolite assays that are not easily obtained from contract labs.

描述（由申请人提供）：此ORIP共享的仪器提案要求资金购买应用的生物系统Sciex QTRAP 6500（R）三倍四倍 /线性离子陷阱质量光谱仪，水域的纯净（R）超压力液相色谱系统和生物量的Biocrates Biost Biost Biost Biost biost biolite biolite sanement in n ebabolite intry sipry in exabolite in the nebabolity in the nebabolity insed kit。在匹兹堡大学，新成立的蛋白质组学和代谢组学中心将几乎用于量化五个化合物的代谢，这些代谢已被识别为识别Qubab的元素，总体而言，通过使九名调查人员能够优化对有价值的样本进行了良好控制的内部分析，从而大大增强了该工具的收购，从而大大增强了7个NIH资助的项目。三倍四极杆LC-MS/MS是该中心的必不可少的服务，而一个五年的热渔船量子Ultra是该设施中这种类型的唯一仪器。该唯一的仪器配置为采用纳米流分离和微小毛细管色谱柱的蛋白质组学分析。正如配置一样，该仪器不适合使用微流隔离和狭窄的孔柱的代谢物测定。此外，将此系统操作作为“双用药”仪器是不切实际的，该仪器在蛋白质和代谢物分析之间切换而不会导致由于大量切换停机而导致生产率损失。由于这些原因，很难满足代谢物定量所需的当前和增长。多个NIH资助的研究人员已诉诸于将样本发送到合同实验室的拼凑而成，这些实验室提供有限的测定功能和可变结果。因此，此应用程序的原因是：1）提供 内部代谢物定量能力至关重要； 2）实现不容易从合同实验室获得的定制代谢物测定法的开发和测试。 Relevance The overall impact of this new instrumentation to human is significant, as 9 independent researchers at the University of Pittsburgh (UP) including members of Children's Hospital (CH), and the University of Pittsburgh Cancer Institute (UPCI) require in-house research tools that are needed to improve and advance NIH funded research into the basic biology of energy metabolism, fatty acid oxidation, mitochondrial metabolism, and phenylalanine toxicity.匹兹堡大学目前尚未提供基于LC-MS/MS的代谢分析。获得AB Sciex QTRAP 6500（R）三倍四倍 /线性离子陷阱质谱仪的获取将首次使这些研究人员和受训者访问此类仪器，并允许在控制良好的条件下完全补充代谢物概况数据。 公众相关性：该orip共享的仪器提案要求资金购买应用的生物系统Sciex QTRAP 6500（R）三倍四倍 /线性离子陷阱质谱仪，水域的生动（R）超压液体色谱系统和生物元素元（TM）Metidq（TM）代谢物分析包。该乐器将安置在匹兹堡大学的生物医学质谱中心；新创建的蛋白质组学和代谢组学校园中心。该集成的LC-MS/MS系统将几乎专门用于定量五个化合物类别的代谢产物，并为12位NIH资助的赠款提供了基本数据，这些赠款授予了9名对基于质谱的代谢物测量的研究者，这些研究人员的需求不断增长。此应用程序的主要原因是：1）提供急需的内部代谢物定量能力； 2）实现不容易从合同实验室获得的定制代谢物测定法的开发和测试。