CV7000

CV7000

• 批准号: 8640626
• 负责人: TOBIAS MEYER
• 金额: $ 59.93万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-01 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8640626
• 关键词: 12 year old; Address; Biosensor; Cell Cycle; Cell Differentiation process; Cell Nucleus; Cell Shape; Cell membrane; Cell physiology; Cells; Fluorescence Microscopy; Funding; Image; Laboratories; Life; Manufacturer Name; Methods; Microscopy; Modeling; Monitor; Movement; N.I.H. Research Support; Organelles; Principal Investigator; Proteins; Request for Proposals; Research; Resource Sharing; Services; Signal Transduction; Signaling Protein; System; Time; United States National Institutes of Health; Universities; base; cell injury; cell motility; cellular imaging; chromatin remodeling; interest; medical schools; research study

PROJECT SUMMARY/ABSTRACT This proposal requests funds to purchase a cutting edge Wako CV7000 High-Content Confocal Imaging system to be shared by eleven NIH-funded Principal Investigators at Stanford University School of Medicine. The new system has two purposes: it replaces a leased automated epifluorescence imaging system ImageXpress micro XL that is heavily used and it adds a critical new capability to the Stanford campus to do automated confocal imaging. The laboratories of Tobias Meyer, James Ferrell, Kang Shen, Thomas Wandless, Steven Artandi, Marius Wernig, Karlene Cimprich, Thomas Quertermous, Michael Lin, Rajat Rohatgi, and Matthew Scott are located in close proximity and share a common interest in microscopy-based dynamic analysis of cellular systems to uncover and understand the cell cycle, cell differentiation, cell damage, cell migration and other dynamic cellular processes. Central to the research in our laboratories is the use of long-term, live-cell, fluorescence microscopy to perturb, monitor and quantify dynamic changes in cellular processes. Specifically, we use methods that require automated tracking of cells, monitoring of changes in cell shape and cell polarization, of organelle distribution and of chromatin remodeling and of changes in local signaling. As part of our projects, we also need to visualize and quantify induced expression and degradation of regulatory and marker proteins as well as the movement of signaling proteins and biosensors to and from the nucleus, plasma membrane and other cellular compartments. Last year, when the manufacturer discontinued all service support for our two heavily-used, 12- year old automated ImageXpress imaging systems, we were able to obtain a one-time lease on a newer-model ImageXpress Micro XL until August 2013. This loaner system is heavily used every day 24/7 since many of our experiments take 1-3 days of continuous multi-well imaging. The advanced high-throughput, live-cell imaging capabilities of the Wako CV7000 will replace this loaned ImageXpress Micro XL and, with the critical addition of confocal capabilities, also significantly enhance the NIH-supported research in our laboratories by providing a shared resource to address our increasing microscopy needs.

项目概要/摘要 该提案要求资金购买先进的 Wako CV7000 高内涵共聚焦 斯坦福大学 11 名 NIH 资助的首席研究员将共享成像系统 大学医学院。新系统有两个目的：它取代了租赁的系统 自动落射荧光成像系统 ImageXpress micro XL 被广泛使用，它 为斯坦福大学校园增加了一项重要的新功能，可以进行自动共焦成像。这 Tobias Meyer、James Ferrell、Kang Shen、Thomas Wandless、Steven Artandi 的实验室， Marius Wernig、Karlene Cimprich、Thomas Quertermous、Michael Lin、Rajat Rohatgi 和 Matthew Scott 距离很近，并且对基于显微镜的技术有着共同的兴趣 细胞系统的动态分析，以揭示和理解细胞周期、细胞 分化、细胞损伤、细胞迁移和其他动态细胞过程。中心到 我们实验室的研究是使用长期的活细胞荧光显微镜来 干扰、监测和量化细胞过程的动态变化。具体来说，我们使用 需要自动跟踪细胞、监测细胞形状和细胞变化的方法 极化、细胞器分布、染色质重塑和局部变化 发信号。作为我们项目的一部分，我们还需要可视化和量化诱导表达和 调节蛋白和标记蛋白的降解以及信号蛋白的运动 以及往返于细胞核、质膜和其他细胞区室的生物传感器。 去年，当制造商停止为我们的两个频繁使用的 12- 一年前的自动化 ImageXpress 成像系统，我们能够获得一次性租赁 2013 年 8 月之前的较新型号 ImageXpress Micro XL。该借用系统被大量使用 每天 24/7，因为我们的许多实验需要 1-3 天的连续多孔成像。 Wako CV7000 先进的高通量活细胞成像功能将取代 这借用了 ImageXpress Micro XL，并且通过添加重要的共焦功能，还 通过提供共享的信息，显着增强 NIH 支持的实验室研究 满足我们日益增长的显微镜需求的资源。