Cellular Imaging Core

细胞成像核心

基本信息

批准号：
8507254
负责人：
Vittorio Gallo
金额：
$ 20.44万
依托单位：
CHILDREN'S RESEARCH INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8507254
关键词：
3-Dimensional Adopted Adult Affinity Age Amygdaloid structure Animal Model Animals Area Astrocytes Autistic Disorder Binding Biochemical Biological Biology Biometry Blood - brain barrier anatomy Books Brain Cell Culture Techniques Cell Death Cell Line Cell Transplantation Cells Characteristics Child Coculture Techniques Collaborations Computer Interface DNA Data Data Analyses Defect Detection Development Devices Dimensions Direct immunofluorescence Disease Dyes Electron Microscope Electron Microscopy Electroporation Embryo Embryonic Development Equipment Event Fluorescence Fragile X Syndrome Freezing Funding Genetic Materials Genomics Goals Grant Growth Factor Hormones Hour Human Human Resources Image Image Analysis Imaging technology Immigration Immunofluorescence Microscopy In Situ Indirect Immunofluorescence Informatics Institutes Journals Laser Scanning Confocal Microscopy Lasers Life Light Lighting Mediating Mental Retardation and Developmental Disabilities Research Centers Messenger RNA Metabolism Methods Microscope Microscopic Mitotic Molecular Profiling Nature Neuroglia Neurons Neurosciences Neurotransmitter Receptor Oligodendroglia Optics Paraffin Embedding Pathology Patients Phenotype Photons Phototoxicity Population Preparation Procedures Process Proteomics Protocols documentation Publications Publishing Quantum Dots Reagent Reporter Research Research Institute Research Personnel Research Project Grants Resolution Rodent Role Sampling Scanning Scientist Services Signal Pathway Signal Transduction Pathway Site Slice Source Specimen Staging System Staining method Stains Stem cells Synapses System Techniques Thick Time Tissue Sample Tissue Stains Tissues Tracer Transfection Tube Viral Proteins Work Writing animal tissue cell fixing cell motility cellular imaging design detector experience fluorescence imaging gamma-Aminobutyric Acid genetic analysis image registration imaging modality in utero in vivo innovation instrument instrumentation interest knock-down laser capture microdissection light microscopy light scattering medical schools migration mouse model nerve stem cell neuroblast neurotoxic optical disc overexpression photomultiplier postnatal progenitor reconstruction relating to nervous system repaired research study stem tissue culture tissue/cell preparation tool trafficking transmission process

项目摘要

E. Justification A key goal of this Core is to provide access to state-of-the-art imaging technologies to researchers at CN and GWU to perform studies on cells and animal models in this area of IDDR. In addition, the Cellular Imaging and Analysis Core assist investigators in their studies on a variety of children's diseases through the expertise of the staff and the affiliated users. The services offered include assistance in 1) preparation of samples and examination of the cells and tissues by light microscopy and EM (see projects* 1-6), 2) quantitative analysis of the data obtained for each project and 3) obtaining cells and tissue samples to carry out genomics/proteomics analysis (see project#6). For these services we make use of dedicated staff in the Core and also through the communal participation of the users with specific expertise (see section E.2, 3). Additionally, we work together with other Cores including, the Genomics and Proteomics and the Biostatistics and Informatics Cores. Use of the recently acquired laser capture microscope (see section E.6.) has enhanced our ability for targeted extraction of diseased cells and tissues from animal or patient materials further enhancing the analysis of these materials with the help of the Genomics and Proteomics Core. Additionally the services provided for establishing neural cell cultures and cell lines have been valuable for subsequent analysis of these purified populations of cells for quantitative analysis of their molecular profiles through genomic and proteomics analysis. In the coming years, we will increase the support our Core provides in carrying out live imaging and quantitative analysis of imaging data. To this end, we have a new Director with extensive and outstanding experience in both these aspects and have hired a new Core Manager who has background and several years of experience in performing quantitative image analysis. In addition, we have started working closely with the Biostatistics and Informatics Core to enable the users to appropriately design and carry out suitable statistical analysis of their data. We are also working in close collaboration with other IDDRCs nationwide - one example of which is the work that Drs. Corbin and Huntsman's have been performing in collaboration with the IDDRC at the Kennedy Krieger Institute at John's Hopkins Medical School. Their work with Dr. Walter Kaufmann (an expert clinician and scientist in the area of Fragile X Syndrome) makes use of the Cellular Imaging Core and has recently resulted in a publication on inhibitory synaptic defects in a mouse model of Fragile X Syndrome (Olmos-Serano et al., 2010).

E. 理由该核心的一个关键目标是为 CN 和 GWU 的研究人员提供最先进的成像技术，以对 IDDR 领域的细胞和动物模型进行研究。此外，细胞成像和分析核心通过工作人员和附属用户的专业知识协助研究人员对各种儿童疾病进行研究。提供的服务包括协助 1) 制备样品并通过光学显微镜和 EM 检查细胞和组织（参见项目* 1-6），2) 对每个项目获得的数据进行定量分析，以及 3) 获取细胞和组织样本进行基因组学/蛋白质组学分析（参见项目#6）。对于这些服务，我们利用核心的专门人员，并通过具有特定专业知识的用户的共同参与（参见 E.2、3 节）。此外，我们还与其他核心合作，包括基因组学和蛋白质组学以及生物统计学和信息学核心。使用最近购买的激光捕获显微镜（参见第 E.6 节）增强了我们从动物或患者材料中定向提取患病细胞和组织的能力，在基因组学和蛋白质组学核心的帮助下进一步增强了对这些材料的分析。此外，为建立神经细胞培养物和细胞系提供的服务对于这些纯化的后续分析非常有价值。通过基因组和蛋白质组学分析对细胞群进行分子图谱定量分析。未来几年，我们将增加核心对实时成像和成像数据定量分析的支持。为此，我们聘请了一位在这两个方面都拥有丰富而出色经验的新总监，并聘请了一位具有多年定量图像分析背景和经验的新核心经理。此外，我们已开始与生物统计和信息学核心密切合作，使用户能够对其数据进行适当的设计和适当的统计分析。我们还与全国其他 IDDRC 密切合作 - 其中一个例子是 Drs. 的工作。 Corbin 和 Huntsman 一直与 IDDRC 在约翰霍普金斯医学院肯尼迪克里格研究所合作进行表演。他们与 Walter Kaufmann 博士（脆性 X 综合征领域的专家临床医生和科学家）合作，利用细胞成像核心，最近发表了一篇关于脆性 X 综合征小鼠模型中抑制性突触缺陷的出版物（Olmos-塞拉诺等人，2010）。