FLOW CYTOMETRY AND CELL SORTING

流式细胞术和细胞分选

• 批准号: 8436444
• 负责人: William L. Carroll
• 金额: $ 5.56万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-03-01 至 2018-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8436444
• 关键词: Basic Science; Cancer Center Support Grant; Cell Separation; Cells; Cellular biology; Clinic; Clinical; Cloning; Color; Communities; Consultations; Data; Dimensions; Equipment; Faculty; Flow Cytometry; Fluorescence; Growth; Hand; Hour; Human; Human Resources; Individual; Institutes; Institution; Instruction; Kaplan Cancer Center; Laboratories; Lasers; Malignant Neoplasms; Modality; Patient Care; Policies; Property; Research; Research Personnel; Resource Sharing; Resources; Running; Schedule; Services; Sorting - Cell Movement; Speed; Sterility; Students; System; Technical Expertise; Technology; Training; Training Activity; Training Technics; Validation; anticancer research; cancer therapy; cell type; design; instrument; light scattering; meetings; member; pre-doctoral; research study; virtual

PROJECT SUMMARY (See instructions): The Flow Cytometry & Cell Sorting Shared Resource is a critical technology for members of the NYU Cancer Institute (NYUCI). Flow Cytometry allows analysis of the light scattering and fluorescence properties of individual cells and the rapid, statistically detailed analysis of 10,000s of cells. The data on individual cells is retained, and subpopulations can be identified in multiple dimensions, showing distinct phenotypical cell types. Cells with particular fluorescence profiles can also be purified and collected for growth or further analysis using the cell sorting. The Flow Cytometry & Cell Sorting Shared Resource offers users who cannot afford and maintain their own Sorters and Analyzers access to this technology, as well as the technical expertise required to design experiments and evaluate experimental results. The facility has a Beckman Coulter MoFlo sorter with 4 lasers and up to 9 colors of Fluorescence, a Becton Dickinson 5-laser 14-color ARIA II cell sorter in a BSL-2 biosafety enclose for primary human cell sorting, an iCyt/SONY Reflection parallel cell sorter, as well as a 5-laser 17-color Becton Dickinson LSRII analyser. The shared resource also has a Becton Dickinson FACScalibur and FACScan analyzer. All cell sorting experiments are performed by highly-trained shared resource staff, and analyzers are run by investigators who have been trained by the shared resource staff. The scientific director also participate in training activities including individual consultations, public seminars and hands on training of users. The facility performed 3,887 hours of analysis and 3,111 hours of sorting during the most recent 12 month period.

项目摘要（参见说明）： 流式细胞术和细胞分选共享资源对于纽约大学癌症研究所 (NYUCI) 的成员来说是一项关键技术。流式细胞术可以分析单个细胞的光散射和荧光特性，并对数以万计的细胞进行快速、统计详细的分析。保留单个细胞的数据，并且可以在多个维度上识别亚群，显示不同的表型细胞类型。还可以纯化并收集具有特定荧光谱的细胞以进行生长或使用细胞分选进行进一步分析。流式细胞术和细胞分选共享资源为无法负担和维护自己的分选仪和分析仪的用户提供了访问该技术的机会，以及设计实验和评估实验结果所需的技术专业知识。该设施拥有配备 4 种激光和多达 9 种荧光颜色的 Beckman Coulter MoFlo 分选机、Becton Dickinson 5 激光 14 色 ARIA II 细胞分选机，位于 BSL-2 生物安全外壳中，用于初级人体细胞分选、iCyt/SONY Reflection平行细胞分选仪，以及 5 激光 17 色 Becton Dickinson LSRII 分析仪。共享资源还有 Becton Dickinson FACScalibur 和 FACScan 分析仪。所有细胞分选实验均由训练有素的共享资源工作人员进行，分析仪由经过共享资源工作人员培训的研究人员运行。科学总监还参与培训活动，包括个人咨询、公共研讨会和用户实践培训。在最近 12 个月期间，该设施执行了 3,887 小时的分析和 3,111 小时的分类。