Flow Cytometry

流式细胞术

• 批准号: 10454163
• 负责人: Jordi Ochando
• 金额: $ 13.82万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10454163
• 关键词: ATAC-seq; Antigens; Area; Basic Science; Biological; Cancer Center Support Grant; Cancer Patient; Cancer Research Project; Cell Separation; Cell surface; Cells; Cellular Indexing of Transcriptomes and Epitopes by Sequencing; Cellular biology; ChIP-seq; Characteristics; Clinical Research; Complex; Consultations; Data Analyses; Development; Diagnosis; Disease; Doctor of Philosophy; Equipment; Flow Cytometry; Flow Cytometry Shared Resource; Fluorescence-Activated Cell Sorting; Health; Human; Human Resources; Institutes; Knowledge; Laboratories; Lead; Malignant Neoplasms; Methods; Mind; Mission; Molecular; Mus; Process; Productivity; Proteomics; Research Personnel; Research Project Grants; Resource Sharing; Sampling; Services; Site; Specialist; Technology; Training; Translational Research; base; cancer cell; clinical investigation; cost effectiveness; education cost; improved; innovation; instrumentation; lipidomics; medical schools; member; metabolomics; pathogen; programs; recruit; transcriptome sequencing

PROJECT SUMMARY/ABSTRACT The Tisch Cancer Institute (TCI) Flow Cytometry Shared Resource Facility (FCSRF) provides TCI members with access to state-of-the-art technology and expertise for the successful development of scientific research projects. Flow cytometry is a powerful technology that enables researchers to study simultaneous multiparametric characteristics of thousands of cells per second to decipher complex cellular and molecular processes in health and disease. The FCSRF comprises the institutional Flow Cytometry Core and two TCI satellite sites. The FCSRF is equipped with six analyzers and six cell sorters contained in biosafety level 2+ areas dedicated to isolate non-fixed human samples and mouse cells infected with class II pathogens. In addition, two satellite sites are equipped with four analyzers. Staffed with seven flow cytometry specialists committed at 100% to this facility, the SRF provides operational, training, and consultation services covering all aspects of flow cytometry including fundamental cell biology principles, analyses, isolation single cells from cancer patients, and identification of over thirty different cell surface/intracellular antigens on the same cell using the revolutionary Aurora platform. The FCSRF also offers services for fundamental applications, such as single cell isolation, to support innovative and complex biological studies involving RNA-seq, ChIP-seq, ATAC- seq, and CITE-seq together with proteomics, metabolomics, and lipidomics profiling. With this in mind, our mission is to provide the necessary instrumentation and expertise regarding flow cytometry analyses and cell sorting so that TCI laboratories improve human cancer knowledge, diagnosis, and treatment through basic sciences, translational, and clinical research.

项目概要/摘要 蒂施癌症研究所 (TCI) 流式细胞术共享资源设施 (FCSRF) 为 TCI 成员提供 能够获得最先进的技术和专业知识，以成功开展科学研究 项目。流式细胞术是一项强大的技术，使研究人员能够同时研究 每秒数千个细胞的多参数特征，以破译复杂的细胞和分子 健康和疾病的过程。 FCSRF 包括机构流式细胞术核心和两个 TCI 卫星站点。 FCSRF配备了生物安全2+级的六台分析仪和六台细胞分选仪 专门用于分离非固定人类样本和感染 II 类病原体的小鼠细胞的区域。在 此外，两个卫星站点配备了四台分析仪。拥有七名流式细胞仪专家 SRF 100% 致力于该设施，提供涵盖所有方面的运营、培训和咨询服务 流式细胞术的各个方面，包括基本细胞生物学原理、分析、从细胞中分离单细胞 癌症患者，并鉴定同一细胞上三十多种不同的细胞表面/细胞内抗原 使用革命性的 Aurora 平台。 FCSRF 还为基础应用提供服务，例如 单细胞分离，支持涉及 RNA-seq、ChIP-seq、ATAC 的创新和复杂的生物学研究 seq、CITE-seq 以及蛋白质组学、代谢组学和脂质组学分析。考虑到这一点，我们的 使命是提供有关流式细胞术分析和细胞分析的必要仪器和专业知识 分类，以便 TCI 实验室通过基础知识提高人类癌症知识、诊断和治疗 科学、转化和临床研究。