Flow Cytometry

流式细胞术

• 批准号: 9754077
• 负责人: Jordi Ochando
• 金额: $ 17.04万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9754077
• 关键词: Antigens; Area; Basic Science; Biological; Biological Assay; Cancer Center Support Grant; Cancer Patient; Cell Cycle; Cell Separation; Cell surface; Cells; Characteristics; Clinical Research; Complex; Consultations; Cytometry; Development; Disease; Doctor of Philosophy; Ensure; Equipment; Flow Cytometry; Flow Cytometry Shared Resource; Goals; Health; Human; Institutes; Knowledge; Laboratories; Malignant Neoplasms; Methods; Mind; Mission; Molecular; Mus; Personnel Recruitments; Process; Productivity; Research; Research Personnel; Research Project Grants; Resource Sharing; Resources; Sampling; Services; Site; Specialist; Technology; Training; Translational Research; anticancer research; cancer cell; cancer diagnosis; cancer therapy; clinical investigation; cost effectiveness; experimental study; improved; innovation; instrumentation; meetings; member; metabolomics; new technology; pathogen; transcriptome sequencing

ABSTRACT – FLOW CYTOMETRY SHARED RESOURCE Shared Resource Leader: Jordi Ochando, PhD The Tisch Cancer Institute (TCI) Flow Cytometry Shared Resource Facility (FCSRF) provides TCI members access to state-of-the-art technology and expertise for the successful development of scientific research projects. Flow cytometry is a powerful technology that enables researchers to study simultaneous multiparametric characteristics of thousands of cells per second to decipher complex cellular and molecular processes in health and disease. The FCSRF offers services for routine applications such as single cell isolation to rapidly isolate live cells conduct more innovative and complex biological studies such as RNAseq, metabolomics, and lipidomics assays. The FCSRF is comprised of the institutional Flow Cytometry Shared Resource Facility and two TCI satellite sites. The FCSRF is equipped with 4 analyzers, 2 mouse cell sorters, 3 cell sorters contained in biosafety level 2+ areas dedicated to isolate non-fixed human samples and mouse cells infected with class II pathogens, and 1 mass cytometer (CyTOF). In addition, two satellite sites are equipped with 4 analyzers. Staffed with 7 flow and mass cytometry specialists committed at 100% to this facility, the SRF provides operational, training, and consultation services covering all aspects of flow cytometry including cell cycle analysis, isolation single cells from cancer patients, and identification of over 30 different cell surface/intracellular antigens on the same cell using mass cytometry. With this in mind, our mission is to provide the necessary instrumentation and knowledge regarding flow/mass cytometry and cell sorting so that Tisch Cancer Institute investigators improve human cancer diagnosis and treatment through basic sciences, translational, and clinical research.

摘要 – 流式细胞术共享资源 共享资源负责人：Jordi Ochando 博士 蒂施癌症研究所 (TCI) 流式细胞术共享资源设施 (FCSRF) 为 TCI 成员提供 获得最先进的技术和专业知识以成功开展科学研究 流式细胞术是一项强大的技术，使研究人员能够同时进行研究。 每秒数千个细胞的多参数特征，以破译复杂的细胞和分子 FCSRF 为单细胞等常规应用提供服务。 快速分离活细胞进行更多创新和复杂的生物学研究，例如RNAseq， FCSRF 由机构流式细胞术共享组成。 资源设施和两个 TCI 卫星站点配备了 4 台分析仪、2 台小鼠细胞分选仪、3 台。 生物安全 2+ 级区域内包含的细胞分选机，专用于分离非固定人类样本和小鼠 感染 II 类病原体的细胞和 1 个质谱流式细胞仪 (CyTOF) 此外，还有两个卫星站点。 配备 4 台分析仪，配备 7 名流式细胞术和质谱流式细胞术专家，100% 致力于此。 SRF 提供涵盖流式细胞术各个方面的操作、培训和咨询服务 包括细胞周期分析、从癌症患者身上分离单细胞以及鉴定 30 多种不同的细胞 考虑到这一点，我们的使命是使用质谱流式技术检测同一细胞上的细胞表面/细胞内抗原。 提供有关流式/质量细胞术和细胞分选的必要仪器和知识，以便 蒂施癌症研究所的研究人员通过基础科学改善人类癌症的诊断和治疗， 转化和临床研究。