CD4 T cell responses to Chlamydia infection

CD4 T 细胞对衣原体感染的反应

基本信息

批准号：
9232975
负责人：
STEPHEN J MCSORLEY
金额：
$ 38.47万
依托单位：
UNIVERSITY OF CALIFORNIA AT DAVIS
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-03-01 至 2020-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9232975
关键词：
Adjuvant Affect Animal Model Antibiotic Therapy Antigens Bacteria Bacterial Antigens Bacterial Infections Blindness CD4 Positive T Lymphocytes Cells Cellular biology Centers for Disease Control and Prevention (U.S.)Cervix Uteri Chlamydia Chlamydia Infections Chlamydia trachomatis Clinical Research Consensus Data Development Disease Ectopic Pregnancy Female Flagellin Foundations Generations Genital system Health Heterogeneity Human Imagery Immune Immunity Immunization Incidence Individual Infection Infertility Knowledge Laboratories MHC Class II Genes Mediating Mediator of activation protein Memory Modeling Mucous Membrane Mus Nature Pathology Pelvic Inflammatory Disease Population Primary Infection Process Reagent Regulatory T-Lymphocyte Reporting Route Scientist Sexually Transmitted Diseases Specificity Surface Testing Tissues Vaccines Woman adaptive immune response aged chronic pelvic pain memory CD4 T lymphocyte mouse model public health relevance reproductive reproductive tract response secondary infection sexually active young woman

项目摘要

DESCRIPTION (provided by applicant) Chlamydia infections are a cause of blindness worldwide and a major cause of sexually transmitted disease in the US. Greater understanding of protective immunity to Chlamydia at mucosal surfaces is urgently required if an effective vaccine is to become a reality. Our application will examine fundemental aspects of protective CD4 T cells during infection of the female reproductive tract mucosa. Our experimental approach is unique in that it utilizes a natural route of infection, uses an established model where CD4 T cells participate in bacterial clearance, and allows for direct visualization of endogenous Chlamydia-specific CD4 T cells using reagents that were recently developed in our laboratory. Our application specifically proposes to, (i) examine CD4 T cell heterogeneity and the contribution of these heterogeneous responses to protection and pathology, (ii) examine whether antibiotic-treatment adversely affects the development of protective TRM cells in the reproductive tract mucosa, (iii) determine whether the use of flagellin can enhance moderate protective immunity elicited by the single antigen PmpG1. Understanding each of these issues will increase our knowledge of Chlamydia-specific CD4 T cell biology and may be vitally important for the generation of a new Chlamydia vaccine.

描述（由应用提供）衣原体感染是全球失明的原因，也是美国性传播疾病的主要原因。如果有效的疫苗成为现实，则迫切需要对粘膜表面上对衣原体的免疫力有更多的了解。我们的应用将检查女性生殖道粘膜感染期间受保护的CD4 T细胞的基金方面。我们的实验方法是独一无二的，因为它利用了自然的感染途径，使用既定模型，其中CD4 T细胞参与细菌清除率，并允许使用最近在我们的实验室中开发的试剂直接可视化内源性衣原体特异性CD4 T细胞。我们的应用专门提出了（i）检查CD4 T细胞异质性以及这些异质反应对保护和病理学的贡献，（ii）检查抗生素治疗是否不利影响生殖道粘膜中生殖型粘膜（III）的生殖型TRM细胞的发展，（III）是否可以增强型号的免疫力PM。了解这些问题中的每一个都将增加我们对衣原体特异性CD4 T细胞生物学的了解，并且对于生成新的衣原体疫苗可能至关重要。