Generation of a TCR transgenic mouse to study Chlamydia genital tract infection

产生 TCR 转基因小鼠以研究衣原体生殖道感染

基本信息

批准号：
8867708
负责人：
STEPHEN J MCSORLEY
金额：
$ 23.02万
依托单位：
UNIVERSITY OF CALIFORNIA AT DAVIS
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-02-01 至 2017-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8867708
关键词：
Address Affect Antigen-Presenting Cells Antigens Bacteria Bacterial Infections Blindness CD4 Positive T Lymphocytes Cells Cellular biology Centers for Disease Control and Prevention (U.S.)Cervix Uteri Chlamydia Chlamydia Infections Chlamydia muridarum Chlamydia trachomatis Clinic Communicable Diseases Consensus Dendritic Cells Development Disease Disease Notification Ectopic Pregnancy Effector Cell Event Family Planning Female Generations Genital system Heterogeneity Imagery Immunity Incidence Infection Infertility Kinetics Knowledge Memory Modeling Nature Organism Pathology Pelvic Inflammatory Disease Reagent Reporting Reproductive Health Reproductive Tract Infections Research Route Scientist Sexually Transmitted Diseases Staging Surface System T cell response T memory cell T-Cell Activation Techniques Testing Th1 Cells Time Tissues Transgenic Mice Transgenic Organisms Vaccines Woman Women&apos s Health chronic pelvic pain design genital infection immune clearance memory CD4 T lymphocyte mouse model pathogen public health relevance reproductive response tool young woman

项目摘要

DESCRIPTION (provided by applicant): Chlamydia infections are a major cause of sexually transmitted disease in the US. Greater understanding of protective immunity to Chlamydia at mucosal surfaces is urgently required if an effective vaccine is to become a reality. This application proposes to develop a TCR transgenic mouse to uncover fundamental aspects of mucosal CD4 T cell biology during infection of the reproductive tract with Chlamydia. Our experimental approach is unique in that it will utilize a natural route of infection, an establishe mouse model of infection where CD4 T cells participate in pathogen clearance, and involve direct visualization of pathogen-specific CD4 T cells using reagents and techniques that we will develop. Our application specifically proposes to, (i) identify antigen presenting cell subsets tha drive CD4 T effector cell priming in the reproductive tract, and (ii) define the heterogeneity of te CD4 T cell memory pool required for protective immunity in the upper reproductive tract. Understanding both these issues will be vitally important for the generation of a vaccine against Chlamydia, one of the most important pathogens affecting the reproductive health of young women in the US.

描述（由应用提供）：衣原体感染是美国性传播疾病的主要原因。如果有效的疫苗成为现实，则迫切需要对粘膜表面上对衣原体的保护性免疫的了解。这项应用提出的建议是开发TCR转基因小鼠，以发现粘膜CD4 T细胞生物学在用衣原体感染期间的基本方面。我们的实验方法是独一无二的，因为它将利用自然的感染途径，这是一种建立的小鼠感染模型，其中CD4 T细胞参与病原体清除率，并使用我们将开发的试剂和技术直接可视化病原体特异性CD4 T细胞。我们的应用专门提议（i）确定抗原呈现细胞子集的驱动CD4 t效应细胞在生殖道中的启动，并且（ii）定义了te CD4 T细胞记忆池的异质性，需要在上部生殖小道中保护性免疫。理解这两个问题对于针对衣原体的疫苗产生而言，这是影响美国年轻妇女复制健康的最重要的病原体之一。