Defining the conformational control of nitric oxide synthases by a multipronged approach

通过多管齐下的方法定义一氧化氮合酶的构象控制

• 批准号: 10571224
• 负责人: Changjian Feng
• 金额: $ 8.91万
• 依托单位: UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10571224
• 关键词: Administrative Supplement; Alzheimer' s Disease; Award; Bioinorganic Chemistry; Computer software; Development; Disease; Environment; Equipment; Family; Financial Support; Fourier Transform; Funding; Institution; Investigation; Investments; Methodology; Methods; Molecular Conformation; New Mexico; Nitric Oxide Synthase; Parents; Performance; Pharmacy facility; Protein Dynamics; Proteins; Research; Research Personnel; Resolution; Sampling; Site; Spectroscopy, Fourier Transform Infrared; Speed; Stroke; System; Techniques; United States National Institutes of Health; Universities; biophysical chemistry; college; design; effective therapy; infrared spectroscopy; instrument; instrumentation; novel therapeutics; parent project; protein complex

PROJECT SUMMARY The parent award is on deciphering conformational dynamics of nitric oxide synthase proteins through an integrated approach using advanced spectroscopic techniques. Fourier transform infrared (FT IR) spectroscopy is essential to the proposed studies of short-range conformational sampling (Aims 2 and 3) in the parent project. Here, we request funds to purchase a state-of-the-art Bruker VERTEX 80 FT IR spectrometer to enhance our IR research capabilities. Combining high sensitivity, resolution and speed with enhanced expandability and easy-to-use FT IR software, the VERTEX 80 system is the answer for demanding protein IR research. The high-performance research-grade instrument is designed to be top of the line. The superior capabilities of the system will be necessary for our efforts to develop and optimize our workflow and methods for site-specific IR spectroscopic investigation of local protein dynamics. Our findings will greatly benefit other investigators using IR methodologies for large, complex proteins. Therefore, the requested equipment will help the parent project to exert a sustained, powerful influence on the research fields of biophysics and bioinorganic chemistry. Moreover, the administrative supplement will enhance the scientific environment for NIH-funded investigators at the University of New Mexico, both within and outside of the College of Pharmacy. To our knowledge, no comparable instrumentation exists in educational institutions within the region. Dean Godwin has committed substantial financial support to this supplement request since this is a great investment for the College. Taken as a whole, the proposed supplement will increase the parent award’s overall impact within the original scope and bolster research quality and depth at our institution.

项目概要 家长奖是通过破译一氧化氮合酶蛋白的构象动力学 使用先进的傅里叶变换红外（FT IR）技术的综合方法。 光谱学对于短程构象采样（目标 2 和 3）的拟议研究至关重要 在此，我们请求资金购买最先进的 Bruker VERTEX 80 FT 红外光谱仪 增强我们的红外研究能力，将高灵敏度、分辨率和速度与增强的功能相结合。 VERTEX 80 系统具有可扩展性和易于使用的 FT IR 软件，是要求严苛的蛋白质 IR 的解决方案 高性能研究级仪器的设计是一流的。 该系统的功能对于我们努力开发和优化我们的工作流程和方法是必要的 我们的研究结果将极大地有益于其他领域。 研究人员使用红外方法来研究大型、复杂的蛋白质，因此，所需的设备将有所帮助。 母项目对生物物理学和生物无机研究领域产生持续、强大的影响 此外，行政补充将改善 NIH 资助的科学环境。 新墨西哥大学药学院内外的研究人员。 据了解，该地区的教育机构中没有类似的仪器。 已承诺对该补充请求提供大量财政支持，因为这对 总的来说，拟议的补充将增加家长奖在大学内的整体影响。 原创范围并提高我们机构的研究质量和深度。