Developing a highly specific and sensitive glucagon ELISA kit

开发高度特异性和敏感性的胰高血糖素 ELISA 试剂盒

基本信息

批准号：
8974749
负责人：
XIAOMIN FAN
金额：
$ 22.47万
依托单位：
AVANTGEN, INC.
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-09-01 至 2017-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8974749
关键词：
Address Affect Affinity Amino Acids Antibodies Antibody Affinity Antigen Targeting Antigens B-Lymphocytes Binding Biological Assay Biology Blood Blood Circulation Blood Glucose C-terminal Cells Cleaved cell Custom Cysteine DNA amplification Detection Diabetes Mellitus Ensure Enteroglucagon Enzyme-Linked Immunosorbent Assay Epidemic Exhibits GLP-2 Glucagon Gluconeogenesis Glucose Glycolysis Goals Harvest Hormones Human Hypoglycemia Immunization Immunoglobulin Variable Region Individual Injection of therapeutic agent Insulin Keyhole Limpet Hemocyanin Kinetics Label Libraries Light Liver Measurement Measures Methods Monitor Monoclonal Antibodies Mus N-terminal OGTT Oryctolagus cuniculus Pancreas Pathway interactions Peptides Peripheral Blood Mononuclear Cell Plasma Play Prediabetes syndrome Preparation Process Production Protocols documentation RNA Reagent Relative (related person)Reverse Transcriptase Polymerase Chain Reaction Role Specificity Spleen Splenocyte System Technology Testing Therapeutic Therapeutic Intervention Tissues Yeasts blood glucose regulation carboxyl radical cross reactivity glucagon-like peptide 1 novel peptide hormone phase 1 study proglucagon prototype public health relevance response therapeutic target vector

项目摘要

DESCRIPTION (provided by applicant): With prediabetes and diabetes reaching epidemic proportions, there is an urgent need to identify more effective therapeutics. Diabetes is characterized by elevated levels of the peptide hormone glucagon,, and inappropriate suppression of glucagon during an oral glucose tolerance test. Glucagon plays a major role in up-regulating glucose release from the liver during hypoglycemic conditions, and serves as a key counter-regulatory hormone to insulin. Consequently, glucagon pathways are an attractive target for therapeutic intervention. However, tissue-specific processing of the proglucagon precursor results in the production of several other peptide hormones, such as oxyntomodulin, GLP-1 and GLP- 2, that also affect glucose levels but by different direct or indirect modes of action. The presence of several alternatively cleaved products that also harbor the glucagon sequence along with the low levels (< 50pM) of glucagon in the circulation has hampered efforts to develop a suitable assay for accurate determination of glucagon levels when analyzing potential therapeutic targets. The goal of this proposal is to use AvantGen's novel yeast display system and rabbit monoclonal antibody discovery technology to isolate pM affinity, highly specific anti-glucagon antibody clones to be used to develop a highly specific and sensitive assay reagents.

描述（由申请人证明）：糖尿病和糖尿病的流行比例治疗中的卢克斯耐受性测试在降低糖尿病的情况下从肝脏上释放起来起着主要作用。治疗性干预。循环中的胰高血糖素在分析潜在的治疗靶标时会妨碍胰高血糖水平的测定法。抗体特异性和敏感测定试剂。