Novel platform for isolation of rabbit monoclonal antibodies against modified RNA bases

用于分离针对修饰 RNA 碱基的兔单克隆抗体的新平台

基本信息

批准号：
8841274
负责人：
XIAOMIN FAN
金额：
$ 18.81万
依托单位：
AVANTGEN, INC.
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-09-15 至 2017-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8841274
关键词：
Adenosine Adjuvant Affinity Aliquot Alternative Splicing Antibodies Antibody Affinity Antigen Targeting Antigens Binding Brain Caffeine Cell Separation Cell surface Cells Consensus Sequence Coupled Cues Culture Media Development Disease Dose Ensure Enzyme-Linked Immunosorbent Assay Epigenetic Process Freund&apos s Adjuvant Genetic Variation Goals Guanosine Harvest Health Immune Immune system Immunization Immunoglobulin Variable Region Individual Injection of therapeutic agent Inosine Keyhole Limpet Hemocyanin Label Libraries Messenger RNA Methods Modification Molecular Monitor Monoclonal Antibodies Nature Nervous System Physiology Neuraxis Neurons Nucleotides Oligonucleotides Oryctolagus cuniculus Phase Play Process Property Purines RNA RNA Editing Ribonucleosides Ribonucleotides Role Serum Sorting - Cell Movement Specificity Splenocyte Structure Technology Testing Variant Yeasts base deep sequencing nervous system disorder neurophysiology new technology novel phase 1 study purine response screening tool vector

项目摘要

DESCRIPTION (provided by applicant): RNA editing and post-transcriptional modification is emerging as a key epigenetic mechanism for fine tuning genetic diversity and RNA function of particular importance in the brain. However, while some RNA editing processes have been relatively well described, other RNA modifications are poorly understood and many questions remain about the true significance of these processes to neurological function in health and disease. Recent developments in deep sequencing approaches have highlighted the number and extent of RNA base modifications, but tools with the requisite high specificity are required to understand the role these changes play in neurophysiology at the developmental, regional, cellular and subcellular level. In this proof of concept Phase I study, we propose to use our cutting edge technology that combines the robust nature of the rabbit immune system with the power of yeast display technology to demonstrate the power of this approach to isolate highly specific, high affinity rabbit monoclonal antibodies against three derivatives of adenosine, inosine, N6-methyl-adenosine and N1-methyl-adenosine.

描述（由申请人提供）：RNA 编辑和转录后修饰正在成为微调遗传多样性和大脑中特别重要的 RNA 功能的关键表观遗传机制。然而，虽然一些 RNA 编辑过程已经得到了相对较好的描述，但其他 RNA 修饰却知之甚少，并且关于这些过程对健康和疾病中神经功能的真正意义仍然存在许多问题。深度测序方法的最新发展突出了 RNA 碱基修饰的数量和程度，但需要具有必要的高特异性的工具来了解这些变化在发育、区域、细胞和亚细胞水平的神经生理学中所发挥的作用。在这项概念验证第一阶段研究中，我们建议使用我们的尖端技术，将兔免疫系统的强大特性与酵母展示技术的强大功能相结合，以证明这种方法分离高度特异性、高亲和力的兔单克隆抗体的能力针对腺苷、肌苷、N6-甲基-腺苷和N1-甲基-腺苷的三种衍生物的抗体。