Monitoring Particulate, PAH, Allergen and Microbial Exposures in Asthmatic Kids

监测哮喘儿童的颗粒物、PAH、过敏原和微生物暴露

• 批准号: 9076745
• 负责人: Steven N. Chillrud
• 金额: $ 197.8万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-30 至 2020-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9076745
• 关键词: Acute; Allergic; Archives; Asthma; Binding; Biological Assay; Carbon Black; Cellular Phone; Child; Collection; Communities; Data; Data Coordinating Center; Detection; Development; Devices; Dictyoptera; Elements; Environment; Environmental Risk Factor; Epidemic; Epidemiologic Studies; Epidemiologist; Epidemiology; Future; Gases; Goals; Grant; Heredity; Home environment; Hour; Intervention Studies; Laboratories; Life Cycle Stages; Location; Measurement; Measures; Methods; Miniaturization; Minority; Modeling; Monitor; Morbidity - disease rate; Mus; Optics; Outcome; Particulate; Particulate Matter; Phase; Physiological; Polytetrafluoroethylene; Population; Prevalence; Production; Protocols documentation; Reproducibility; Research; Research Design; Research Personnel; Sampling; Signal Transduction; Sister; Staging; Stream; Symptoms; Testing; Time; Update; Validation; Variant; Wireless Technology; Work; air monitoring; airborne allergen; analytical method; asthmatic; base; cockroach allergen; cohort; data exchange; data visualization; design; diaries; improved; indoor allergen; interest; microbial; microbial allergen; miniaturize; novel; particle; prevent; prototype; public health relevance; pyroglyphid; sensor; spatial temporal variation; stressor; tool; transmission process; ultraviolet; wood smoke

 DESCRIPTION (provided by applicant): In the proposed study, we will integrate a modular array of miniaturized air monitors for use as personal and residential monitors for future epidemiological studies that will test hypotheses on asthma development and asthma exacerbation. Our overall approach is to develop and test (a) a real-time monitor that can serve as a platform to record its own data and data from other monitors to establish spatial and temporal variations of potential acute asthma stressors and (b) a monitor that can collect either spatially- or time- resolved, integrated samples that can be archived for subsequent analysis in the laboratory. Time scales for battery-powered deployments of both the real-time monitors and the integrated sampler could range from 24 hours to a month in duration depending on the study design. Three aims form our proposal. Aim 1) Integrate a modular array of miniaturized air monitors for use as residential and personal monitors. The three monitors that make up our modular array currently exist in various stages of development: a completed monitor that is not fully validated (the Multi-day microAeth(r)), a prototype that will be further miniaturized while expanding functionality (the Archive Monitor) and an off the shelf inexpensive smart phone enabled monitor for airborne particulates (The Airbeam from Aircasting.org). Tri-axial accelerometer and GPS chips are integrated into our personal monitors allowing determinations of location, wearing compliance and activity related parameters. The multi-day microAeth monitor, measures optically absorbant components of particulate matter including black carbon and environmental wood smoke. Bluetooth, WiFi and/or serial connections will allow all sensor data to be logged on the multi-day microAeth(r) to simplify integration and allow all sensor data (personal and residential) to be sent to the study team on a daily basis. We include the Airbeam to show capacity of our platform for integrating data streams from third-party monitors and to show capacity for integrating our sensor data into a pre-existing smart phone app. Aim 2) Refine or develop sensitive analytical methods for a wide suite of asthma-related exposure measurements for archive, integrated samples of PM10, PM2.5 and semi-volatile compounds. The initial suite of measurements to be validated for the archive samples includes airborne allergens (mouse, dust mite and cockroach), PCR based microbial assays, semi-volatile and particle-associated non-volatile PAHs, black carbon, particulate-bound elements, and gravimetric determinations of PM10 and PM2.5 filters. Aim 3) Carry out laboratory and field tests iteratively to identify challenges in using the devices, improve protocols, and determine reproducibility and accuracy of collected data. Field tests will include comparisons to other monitors and feasibility pilots with a cohort of asthmatic children for testing the modular array o air monitors. Feasibility outcomes of interest include data completeness, wearing compliance, and demonstration of the ability to transfer data to future data coordinating centers.

 描述（由适用提供）：在拟议的研究中，我们将整合一系列微型空中监视器，以作为个人和住宅监测器，以供未来的流行病学研究，以测试有关哮喘发育和哮喘恶化的假设。我们的总体方法是开发和测试（a）一个实时监视器，可以作为平台记录其自己的数据和来自其他监视器的数据，以建立潜在的急性哮喘应激源的空间和临时变化，以及（b）可以在空间或时间分析或时间分析的，可以在实验室进行后续分析的监视器。根据研究设计，实时监视器和集成采样器的电池供电部署的时间尺度可能从24小时到一个月不等。三个目标构成了我们的建议。目标1）集成了一个模块化的微型空中监视器，以用作居民和个人监视器。构成我们模块化阵列的三个监视器目前存在于各个开发阶段：尚未得到充分验证的完整监视器（多日微观（R）），该原型将在扩展功能（存档监视器）的同时进一步微型（档案显示器），并启用了货架廉价的智能手机，以实现空中板的智能手机（airborne Conternents airberne airbeam farrialbeam farrieserg.org）。将三轴加速度计和GPS芯片集成到我们的个人监视器中，允许确定位置，磨损合规性和与活动相关的参数。多日微观监测器，可在特定物质的光学吸收性组件中进行测量，包括黑碳和环境木烟。蓝牙，WiFi和/或串行连接将允许在多日微观（R）上记录所有传感器数据，以简化集成，并允许所有传感器数据（个人和居民）每天发送到研究团队。我们包括Airbeam，以显示我们平台的能力，用于集成第三方监视器的数据流，并显示将我们的传感器数据集成到现有的智能手机应用程序中的能力。 AIM 2）针对广泛的哮喘相关暴露测量值来完善或开发敏感的分析方法，用于PM10，PM2.5和半胆固醇化合物的档案，集成样品。要验证的档案样品测量值的初始套件包括空气寄生过敏原（小鼠，尘螨和蟑螂），基于PCR的微生物测定，半挥发性和粒子相关的非挥发性PAH，黑碳，颗粒碳，颗粒元素结合元素，以及PM10和PM2.5文件的粒度测定。目标3）进行实验室和现场测试迭代，以确定使用设备，改进协议并确定收集数据的可重复性和准确性的挑战。现场测试将包括与其他监视器的比较，以及可行性飞行员与一群哮喘儿童进行测试，以测试模块化阵列O空中监视器。感兴趣的可行性结果包括数据完整性，磨损合规性以及将数据传输到未来数据协调中心的能力。