A Resource Center for Tetrahymena Thermophila

嗜热四膜虫资源中心

• 批准号: 9102372
• 负责人: THEODORE G CLARK
• 金额: $ 8.48万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-01 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9102372
• 关键词: Aging; Animal Model; Applied Research; Basic Science; Biological Models; Biological Phenomena; Biological Process; Cells; Cellular biology; Chromosomes; Collection; Communities; Country; Databases; Disease; Eukaryotic Cell; Fees; Fertility; Functional disorder; Funding; Genes; Genetic; Genetic Markers; Genome; Germ Lines; Growth; Health; Housing; Human; Individual; Infectious Agent; Infertility; Knock-in Mouse; Knock-out; Laboratories; Life; Link; Maintenance; Malaria; Malignant Neoplasms; Methodology; Modeling; Molecular Biology; Molecular and Cellular Biology; Neurosecretory Systems; Nuclear Structure; Online Systems; Organism; Plasmids; Proteins; Protocols documentation; Recovery; Relative (related person); Research; Research Personnel; Resources; Services; Study models; Sum; System; Techniques; Tetrahymena; Tetrahymena thermophila; Training; Universities; Update; Vaccine Production; Vaccines; Water; Work; base; cost; direct application; experience; functional genomics; gene replacement; genome database; human disease; improved; influenza virus vaccine; insight; interest; micronucleus; molecular marker; novel; operation; repository; scaffold; therapeutic protein; tool; web site; wiki

DESCRIPTION (provided by applicant): This proposal seeks continued funding for the Tetrahymena Stock Center and ancillary Tetrahymena Genome Database (TGD) in order to maintain their current operations and expand their capabilities as resources for the community at-large. A key model for eukaryotic cell and molecular biology, Tetrahymena thermophila has been instrumental to our understanding of a wide range of biological phenomena with direct relevance to human health and disease including cancer, infertility, aging, and neuroendocrine dysfunction. Tetrahymena has also shown great promise as a platform for protein manufacture including the production of vaccines for influenza, malaria and other infectious agents. Specifically, we aim to 1) maintain genetic stocks in our current facilities and make them available to the scientific community via our simple web-based ordering system; 2) expand the current collection and complete annotation of existing strains; 3) extend the Center's services to include storage and dissemination of useful plasmid constructs, and a new fee-based transformation service that will significantly increase cost recovery; 4) offer short techniques an methodology training sessions to promote the use of Tetrahymena by investigators who are new to the system; 5) promote continued community-based annotation of the Tetrahymena macronuclear genome via the TGD Wiki; 6) establish links between individual strains in the Stock Center collection and relevant gene pages in TGD to simplify identification of useful strains and improve information access for the Center's users. Lastly, as part of the research component of the Stock Center, we will 7) create more efficient strategies for micronuclear transformation and utilize these to generate a panel of small, targeted deletions in all five micronuclear chromosomes. This panel will act as a scaffold on which genetic and molecular markers can be quickly aligned and will strongly support the many functional genomic studies now underway in T. thermophila. The ability to create viable, homozygous deletions of virtually any size is only possible due to the unique dimorphic nuclear structure of Tetrahymena and represents one of many novel features of this important model organism.

描述（由申请人提供）：该提案寻求继续为四膜虫库存中心和辅助四膜虫基因组数据库（TGD）提供资金，以维持其当前的运营并扩大其作为整个社区资源的能力。作为真核细胞和分子生物学的关键模型，嗜热四膜虫有助于我们理解与人类健康和疾病直接相关的广泛生物现象，包括癌症、不孕不育、衰老和神经内分泌功能障碍。四膜虫还显示出作为蛋白质制造平台的巨大前景，包括生产流感、疟疾和其他传染原的疫苗。具体来说，我们的目标是 1) 在我们现有的设施中维护遗传资源，并通过我们简单的基于网络的订购系统将其提供给科学界； 2）扩大现有菌株的收集并完成现有菌株的注释； 3) 扩大中心的服务范围，包括有用质粒构建体的储存和传播，以及新的收费转化服务，这将显着提高成本回收； 4) 提供简短的技术和方法培训课程，以促进刚接触该系统的研究人员对四膜虫的使用； 5) 通过 TGD Wiki 促进四膜虫大核基因组持续基于社区的注释； 6) 在Stock Center收藏中的各个菌株和TGD中的相关基因页面之间建立链接，以简化有用菌株的识别并改善中心用户的信息访问。最后，作为库存中心研究部分的一部分，我们将 7) 创建更有效的微核转化策略，并利用这些策略在所有五个微核染色体中生成一组小的、有针对性的删除。该面板将充当一个支架，可以在其上快速对齐遗传和分子标记，并将大力支持目前在嗜热木霉中正在进行的许多功能基因组研究。由于四膜虫独特的二态性核结构，才有可能产生几乎任何大小的可行的纯合缺失，这代表了这种重要模式生物的许多新特征之一。