A RESOURCE CENTER FOR TETRAHYMENA THERMOPHILA

嗜热四膜虫资源中心

• 批准号: 7392023
• 负责人: THEODORE G CLARK
• 金额: $ 33.54万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7392023
• 关键词: RESOURCE; CENTER; TETRAHYMENA; THERMOPHILA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This proposal seeks to establish a centralized repository for genetically distinct strains of Tetrahymena thermophila, a ciliated protozoan that has served as a key model for studies of eukaryotic cellular and molecular biology for more than 50 years. The stock center will 1) collect, annotate, and store experimentally useful cell lines; 2) establish a database that will provide essential information about these strains to the community at-large; and, 3) supply actively growing cultures to researchers around the world. This resource will provide a safe haven for the roughly 4000 unique strains developed over the past several decades, and at the same time, serve as an important adjunct to the ongoing Tetrahymena genome sequencing project. Activities spawned by this large-scale, NIH-funded sequencing effort are expected to result in the development of many new strains that will benefit greatly from the existence of a centralized storage and distribution facility. In addition to the establishment of a stock center, the proposal also intends to lay the groundwork for creation of a physical map of the Tetrahymena genome. This second goal is an extension of research currently being undertaken in the Principal Investigator's laboratory, and seeks to adapt the Cre-lox recombinase technology to the Tetrahymena system in order to create a panel of mid-sized (~ 4-5 Mb) overlapping deletions spanning the micronuclear genome. This set of specifically targeted, midsized deletions will augment an existing panel of large and small deletions already constructed in our laboratory by random mutagenesis, and will act as a scaffold on which genetic and molecular markers can be quickly aligned. The ability to create viable, homozygous deletions of virtually any size is only possible due to the unique dimorphic nuclear structure of Tetrahymena, and represents a powerful tool that will pave the way for functional genomic studies in this system and enhance the utility of the resource overall. Although a unicellular organism with simple growth requirements, Tetrahymena is endowed with unique genetic properties, and a structural and functional complexity comparable to that of higher metazoa. Not surprisingly, it has contributed enormously to our understanding of a wide range of biological phenomena including many with direct relevance to human health. The resource described in this proposal will ensure the continued use this organism for the benefit of society as a whole.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是中心的机构，不一定是研究者的机构。描述（由申请人提供）：该提案旨在为遗传上不同的嗜热四膜虫菌株建立一个集中储存库，嗜热四膜虫是一种纤毛原生动物，50 多年来一直是真核细胞和分子生物学研究的关键模型。库存中心将1）收集、注释和储存实验上有用的细胞系； 2）建立一个数据库，向广大社区提供有关这些菌株的基本信息； 3) 为世界各地的研究人员提供积极生长的培养物。该资源将为过去几十年开发的约 4000 种独特菌株提供安全港，同时，作为正在进行的四膜虫基因组测序项目的重要辅助手段。这项由美国国立卫生研究院 (NIH) 资助的大规模测序工作所引发的活动预计将导致许多新菌株的开发，这些新菌株将大大受益于集中存储和分配设施的存在。除了建立库存中心外，该提案还打算为创建四膜虫基因组物理图谱奠定基础。第二个目标是首席研究员实验室目前正在进行的研究的延伸，旨在将 Cre-lox 重组酶技术应用于四膜虫系统，以创建一组中等大小（~ 4-5 Mb）重叠删除跨越微核基因组。这套专门针对的中型缺失将增强我们实验室中通过随机诱变构建的现有大型和小型缺失组，并将充当遗传和分子标记可以快速对齐的支架。由于四膜虫独特的二态性核结构，才有可能创建几乎任何大小的可行的纯合缺失，并且代表了一种强大的工具，将为该系统中的功能基因组研究铺平道路，并提高整体资源的利用率。尽管四膜虫是一种生长要求简单的单细胞生物，但它具有独特的遗传特性，其结构和功能的复杂性可与高等后生动物相媲美。毫不奇怪，它为我们理解广泛的生物现象做出了巨大贡献，其中包括许多与人类健康直接相关的现象。本提案中描述的资源将确保继续使用该生物体，造福整个社会。