In-house Small Angle X-Ray Scattering Instrument

自制小角度X射线散射仪

• 批准号: 8825798
• 负责人: Cynthia Wolberger
• 金额: $ 36.55万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-01 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8825798
• 关键词: Area; Arts; Biological; Cancer Biology; Complex; Contract Services; Core Facility; DNA biosynthesis; Data; Data Collection; Data Set; Doctor of Philosophy; Equipment; Financial Support; Funding; Housing; Image; Laboratories; Methods; Mission; Multi-Drug Resistance; Neurons; Public Health; Research Personnel; Resolution; Ribosomes; Roentgen Rays; Science; Shipping; Ships; Signal Transduction; Structure; Synchrotrons; System; Time; Transcriptional Regulation; United States National Institutes of Health; Universities; Wages; X ray diffraction analysis; X-Ray Crystallography; X-Ray Diffraction; beamline; detector; experience; genome integrity; instrument; macromolecule; medical schools; repaired; screening

 DESCRIPTION (provided by applicant): X-ray crystallography and small-angle x-ray scattering (SAXS) are powerful methods for studying biological assemblies and conformational changes in macromolecules that provide information complementary to high-resolution X-ray crystallography and NMR. The Johns Hopkins University has a large group (16 PIs) of NIH-funded investigators in the Schools of Medicine, Public Health, and Arts and Sciences that utilize SAXS and X-ray crystallography to study a range of problems central to the NIH mission. The requested in-house SAXS system will benefit ongoing projects that utilize SAXS while opening the door to studying systems involving macromolecules and complexes that deteriorate and aggregate over time and thus do not survive shipping to synchrotron beamlines for SAXS data collection. The system requested takes advantage of an existing high-brilliance generator housed at the School of Medicine to obtain in-house SAXS data of optimal quality. To maintain high throughput for in-house single- crystal X-ray diffraction data collection, and since we plan t utilize one of two generator ports for SAXS, we also are requesting a Pilatus pixel-array detector to replace the existing image- plate and CCD detectors currently mounted on both ports. In addition to shortening data collection times, a Pilatus detector offers the added benefit of enabling screening of smaller crystals and collecting higher resolution data sets for structure determination efforts. All of the requested equipment will be incorporated into an X-ray diffraction facility that has served as a core facility for over two decades and is staffed by an experienced PhD-level crystallographer. The University has made a substantial financial commitment to this facility and its upgrade, including providing full salary support for the X-ray lab manager, prepared laboratory space, matching funds, and a guarantee of ongoing financial support to cover service contracts and repairs. The equipment will have an immediate and lasting impact on a broad range of areas including cancer biology, DNA replication, ribosome assembly, intracellular signaling, transcriptional regulation, genomic integrity, neuronal function and multidrug resistance.

 描述（由申请人提供）：X 射线晶体学和小角 X 射线散射 (SAXS) 是研究生物组装和大分子构象变化的强大方法，可提供与高分辨率 X 射线晶体学和 NMR 互补的信息。霍普金斯大学在医学院、公共卫生学院以及艺术与科学学院拥有一大批由 NIH 资助的研究人员（16 名 PI），他们利用 SAXS 和 X 射线NIH 任务所要求的内部 SAXS 系统将有利于正在进行的利用 SAXS 的项目，同时为研究涉及大分子和复合物的系统打开了大门，这些系统会随着时间的推移而恶化和聚集，从而无法在运输中幸存。要求的系统利用医学院现有的高亮度发生器来获取最佳质量的内部 SAXS 数据，以保持内部的高吞吐量。单晶 X 射线衍射数据收集，并且由于我们计划利用 SAXS 的两个发生器端口之一，因此我们还要求使用 Pilatus 像素阵列探测器来替换当前安装在两个端口上的现有图像板和 CCD 探测器。除了缩短数据收集时间外，皮拉图斯探测器还具有额外的优势，可以筛选较小的晶体并收集更高分辨率的数据集以进行结构确定工作。作为核心设施已有二十多年的历史，并由经验丰富的博士级晶体学家组成，该大学已对该设施及其升级做出了大量的财务承诺，包括为 X 射线实验室经理、准备实验室提供全额工资支持。该设备将对癌症生物学、DNA 复制、核糖体组装、细胞内信号传导、转录调控等广泛领域产生直接和持久的影响。基因组完整性、神经功能和多药反抗。