Information Processing in the Thermosensory System of Drosophila Melanogaster

果蝇热传感系统的信息处理

基本信息

批准号：
8983363
负责人：
Dominic David Frank
金额：
$ 3.68万
依托单位：
NORTHWESTERN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-07-01 至 2018-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8983363
关键词：
Axon Behavior Behavioral Assay Brain Brain region Calcium Cells Collaborations Communication Detection Drosophila genus Drosophila melanogaster Environment Experimental Designs Genetic Goals Heating Image Imaging technology Instinct Label Laboratories Learning Life Light Maps Mediating Modality Motor Nervous system structure Neurons Neurosciences Optics Organism Pathway interactions Process Sensory Series Stimulus Synapses System Temperature Temperature Sense Test Result Testing Thermoreceptors Training Work anatomical tracing cell type cold temperature fly in vivo information processing insight member neural circuit neurogenetics optogenetics postsynaptic neurons preference programs public health relevance receptor relating to nervous system research study response sensor sensory stimulus sensory system skills stimulus processing tool two-photon

项目摘要

DESCRIPTION (provided by applicant): Defining all principal components of a neural circuit, from sensory transduction to behavior, is one of the fundamental goals of neuroscience. While this presents a monumental challenge for a mammalian brain; the simpler nervous system, rich repertoire of innate behaviors, and unparalleled genetic tractability of Drosophila melanogaster afford a rare opportunity to uncover fundamentals of neural processing. To this end, our laboratory focuses on understanding how the processing of temperature stimuli in the Drosophila brain produces the appropriate aversive and attractive responses. The ability to sense temperature, thermosensation, provides organisms with critical information; yet it is one of the least understood sensory modalities. In Drosophila, rapid temperature changes are detected by dedicated hot and cold temperature receptors in the antenna, with 3 neurons responding to cooling and 3 neurons responding to heating. The axons of these cells terminate in segregated, adjacent 'hot' and 'cold' glomeruli in the brain; forming a topographic map for the representation of temperature, as demonstrated by calcium imaging. It is unclear how this spatial map of activity is processed by higher brain regions to direct behaviors. Here, I propose to map the transformation of temperature information in the Drosophila brain from sensory detection to the triggering of specific motor programs. This work will provide fundamental insights into high- level sensorimotor neuronal computations in a relatively simple system and may help explain how the brain processes all innate behaviors. In conducting these experiments, I will learn how to perform genetically targeted optogenetic stimulation with tandem two-photon optical recording and behavioral assays. Further, I will be trained in executive scientific skills such as experimental design, scientific communication, and professional networking.

定义新闻业对神经科学的行为目标，而这对哺乳动物的大脑提出了巨大的挑战；，我们的实验室重点是果蝇大脑中温度刺激的处理能力和吸引人的反应。重新加热，如钙成像所证明的，在大脑中形成C代表温度的细胞中的轴突。 HRE，我建议将果蝇中的温度从感觉检测到触发特定的运动程序。光学的科学技能，例如实验设计，科学沟通和专业网络。