Rhesus Monkey Oocyte Resources

恒河猴卵母细胞资源

• 批准号: 8454471
• 负责人: CATHERINE A VANDEVOORT
• 金额: $ 50.39万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8454471
• 关键词: Address; Animal Model; Antigen-Presenting Cells; Barker Hypothesis; Biomedical Research; Cattle; Cells; Characteristics; Communities; Cryopreservation; Development; Disease; ES Cell Line; Embryo; Embryonic Development; Ethics; Fertilization; Funding; Genetic Programming; Goals; Growth; Health; Human; In Vitro; Institutes; Knowledge; Macaca mulatta; Methods; Modeling; Molecular; Monkeys; Mus; National Center for Research Resources; National Institute of Child Health and Human Development; National Institute of Environmental Health Sciences; National Institute on Alcohol Abuse and Alcoholism; Oocytes; Ovarian; Ovarian Tissue; Primates; Production; Protocols documentation; Rattus; Reproduction; Reproductive Biology; Research; Research Personnel; Resource Development; Resources; Sampling; Scientist; Services; Source; Techniques; Technology; Tissues; Training; Transgenic Model; Transgenic Organisms; Translational Research; Translations; United States National Institutes of Health; Work; direct application; improved; investigator training; nonhuman primate; offspring; oocyte maturation; programs; protocol development; public health relevance; stem cell therapy

DESCRIPTION (provided by applicant): Because human and nonhuman primates share many unique characteristics of reproduction, especially in regard to follicle selection and function, oocyte maturation and early embryo growth, the rhesus model is especially critical for research in reproduction that can have direct applications to humans, especially in research to determine fetal origins of adult disease. This resource would be a source of oocytes and follicle cells, as well as protocols and training, for investigators that currently utilize or that want to expand their studies into a primate model to facilitate translation to human applications. Furthermore, the lack of a reliable, adequate supply of non-human primate oocytes is a major problem impeding progress in non- human primate models of early embryo development, fertilization, transgenics, embryonic stem cell lines, and production of genetically identical offspring. Improved technologies for in vitro maturation (IVM) and cryopreservation of non-human primate oocytes would help alleviate this shortage and increase the efficiency of research in this important model for human health. This resource will be developed for the purpose of providing services and samples to investigators that are outside of the Primate Centers to facilitate translational research. Use of the resource will likely encompass investigators funded by a range of categorical institutes including NICHD, NCRR, NIEHS, NIA, NIAAA. Providing access to protocols, training and, ultimately, a "bank" of nonhuman primate oocytes, follicles cells, tissues and lysates is essential for the translational research bridge between other animal models and humans. Therefore, the general objectives of this project are to provide protocols and training for continually developing techniques for production of developmentally competent in vitro matured nonhuman primate oocytes and cryopreservation methods for the long-term storage of nonhuman primate oocytes. The long-term goal will be to provide a bank of oocytes and accessory cells as well as ovarian tissue and cell lysates that can be accessed by NIH funded investigators. The specific aims of the project will be 1) Provide current protocols and training for in vitro maturation and cryopreservation of rhesus monkey oocytes; 2) Develop a bank of oocytes and somatic accessory cells (cumulus and granulosa) for distribution as cryopreserved material, fixed material, and lysates for molecular analyses. Fresh oocytes and somatic accessory cells will also be available for shipment; 3) Address the urgent need in both human and nonhuman reproductive biology to enhance oocyte cryopreservation and IVM methods.

描述（由申请人提供）：由于人类和非人类灵长类动物具有许多独特的生殖特征，特别是在卵泡选择和功能、卵母细胞成熟和早期胚胎生长方面，因此恒河猴模型对于可直接应用的生殖研究尤其重要对人类，特别是在确定成人疾病的胎儿起源的研究中。该资源将成为卵母细胞和卵泡细胞以及方案和培训的来源，供目前使用或希望将其研究扩展到灵长类动物模型以促进转化为人类应用的研究人员使用。此外，缺乏可靠、充足的非人灵长类卵母细胞供应是阻碍早期胚胎发育、受精、转基因、胚胎干细胞系和遗传相同后代的产生的非人灵长类动物模型进展的一个主要问题。非人类灵长类卵母细胞体外成熟（IVM）和冷冻保存技术的改进将有助于缓解这一短缺问题，并提高这一人类健康重要模型的研究效率。开发该资源的目的是向灵长类动物中心之外的研究人员提供服务和样本，以促进转化研究。该资源的使用可能包括由 NICHD、NCRR、NIEHS、NIA、NIAAA 等一系列分类机构资助的研究人员。提供方案、培训以及最终的非人类灵长类卵母细胞、卵泡细胞、组织和裂解物的“库”对于其他动物模型和人类之间的转化研究桥梁至关重要。因此，该项目的总体目标是提供协议和培训，以不断开发生产有发育能力的体外成熟非人灵长类卵母细胞的技术，以及长期储存非人灵长类卵母细胞的冷冻保存方法。长期目标是提供卵母细胞和辅助细胞库以及卵巢组织和细胞裂解物，供 NIH 资助的研究人员访问。该项目的具体目标是 1) 提供恒河猴卵母细胞体外成熟和冷冻保存的现行方案和培训； 2) 开发卵母细胞和体细胞辅助细胞（卵丘和颗粒）库，作为冷冻保存材料、固定材料和裂解物进行分子分析。新鲜卵母细胞和体细胞辅助细胞也将可供运输； 3) 解决人类和非人类生殖生物学领域对加强卵母细胞冷冻保存和 IVM 方法的迫切需求。