EPR Detection of Free Radicals in Vascular Disease: Bruker EMXPlus Spectrometer

EPR 检测血管疾病中的自由基：布鲁克 EMXPlus 光谱仪

• 批准号: 8447255
• 负责人: Eric Eugene Kelley
• 金额: $ 30.4万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-15 至 2014-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447255
• 关键词: Biological Models; Cell Membrane Permeability; Cell membrane; Detection; Development; Disease; Disease Progression; Drug Interactions; Electron Spin Resonance Spectroscopy; Fingerprint; Free Radicals; Goals; Gold; In Vitro; Inferior; Institution; Measures; Medical Research; Methodology; Nitrogen; Oxidation-Reduction; Proteins; Reactive Oxygen Species; Research; Research Personnel; Source; Specificity; Spin Trapping; System; Techniques; Technology; Tissues; Toxic effect; Universities; Vascular Diseases; biological systems; in vivo; medical schools; novel strategies; success

DESCRIPTION: Reactive species of oxygen and nitrogen are becoming increasingly appreciated for their impact on the onset and progression of disease. It is therefore imperative for medical research institutions to possess robust free radical research technology. Currently, investigators at the University of Pittsburgh, School of Medicine are relegated to the use of inferior methodologies to critically interrogate free radical species in their model systems. For example, all currently available fluorescent, colorimetric, luminescent and chemiluminescent probes used to detect free radical species demonstrate reproducible results with relatively high sensitivity however, they have significant limitations regarding specificity, the propensity to redox cycle, membrane permeability and toxicity. This being noted, electron paramagnetic resonance (EPR) spectroscopy is the only technique currently available that affords direct identification of free radicals in biological systems and as such remains the gold standard for free radical research. The utility of EPR is exemplified in its capacity to provide specific fingerprint spectra that identify the free radical being measured. Thus, EPR can provide direct detection and identification of stable free radical species. For less stable free radical species, EPR spin trapping techniques permit detection and identification in a multitude of in vitro and in vivo systems. This technique has proven extremely informative for investigating formation and identifying sources of radicals in cellular, tissue and in vivo systems as well as investigating protein radical intermediates and studying drug interactions with cell membranes. To date, investigators in the School of Medicine have limited to no access to this technology. As such, acquisition of an EPR spectrometer would serve to uniquely cultivate the development of current research initiatives, incentivize new collaborative efforts and enhance the potential for success in achieving our overarching goal of developing novel strategies to treat disease.

描述：氧和氮的活性物质因其对疾病发生和进展的影响而受到越来越多的重视。因此，医学研究机构必须拥有强大的自由基研究技术。目前，匹兹堡大学医学院的研究人员只能使用较差的方法来批判性地询问模型系统中的自由基种类。例如，目前用于检测自由基物质的所有可用的荧光、比色、发光和化学发光探针均表现出具有相对高灵敏度的可重复结果，然而，它们在特异性、氧化还原循环倾向、膜渗透性和毒性方面具有显着的局限性。值得注意的是，电子顺磁共振（EPR）光谱是目前唯一可以直接识别生物系统中自由基的技术，因此仍然是自由基研究的黄金标准。 EPR 的实用性体现在它能够提供识别所测量的自由基的特定指纹光谱。因此，EPR 可以直接检测和鉴定稳定的自由基种类。对于不太稳定的自由基种类，EPR 自旋捕获技术允许在多种体外和体内系统中进行检测和识别。事实证明，该技术对于研究细胞、组织和体内系统中自由基的形成和识别来源，以及研究蛋白质自由基中间体和研究药物与细胞膜的相互作用非常有用。迄今为止，医学院的研究人员还无法使用这项技术。因此，购买 EPR 光谱仪将有助于独特地促进当前研究计划的发展，激励新的合作努力，并增强成功实现我们开发新的疾病治疗策略的总体目标的潜力。