National Resource for Mass Spectrom of Biological Macromolecules

国家生物大分子质谱资源库

• 批准号: 8819554
• 负责人: Brian T Chait
• 金额: $ 146.81万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-23 至 2016-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8819554
• 关键词: Achievement; Acquired Immunodeficiency Syndrome; Address; African; African Trypanosomiasis; Alzheimer' s Disease; Anatomy; Animal Model; Antibodies; Automobile Driving; Autophagocytosis; Bacterial Infections; Biological; Biological Process; Biology; Biomedical Research; Blood Circulation; Chronic Cancer Pain; Collaborations; Communities; Complex; Conotoxin; DNA Double Strand Break; Development; Device or Instrument Development; Diabetes Mellitus; Discipline; Disease; Drug Addiction; Education; Evolution; Family suidae; Four-dimensional; Genetic Transcription; Goals; HIV Antibodies; HIV-1; Health; Hepatitis C; Hepatitis C virus; Human; Infection; Inhibitory Synapse; Link; Lymphoma; Mass Spectrum Analysis; Mediator of activation protein; Methodology; Methods; Modeling; Needles; Nerve Degeneration; Neuropeptides; Noise; Non-Insulin-Dependent Diabetes Mellitus; Nuclear Pore Complex; Nucleotide Excision Repair; Parasites; Parkinson Disease; Pathway interactions; Peptides; Peripheral; Physiology; Preparation; Problem Solving; Proteins; Proteomics; Resources; Sampling; Scientist; Services; Signal Transduction; Simplexvirus; Solutions; Staphylococcus aureus; Structure; System; Techniques; Technology; Testing; Therapeutic; Toxin; Training; Trypanosoma; Tumor Suppressor Proteins; Type III Secretion System Pathway; Viral; Work; amyloid formation; base; human CDK3 protein; improved; instrumentation; islet; macromolecule; novel; particle; protein complex; research and development; response; success; tool

DESCRIPTION (provided by applicant): It is proposed to continue to operate and extend the cutting edge capabilities of the National Resource for the Mass Spectrometric Analysis of Biological Macromolecules for years 40 through 44. Emphasis will be placed on developing mass spectrometric technology for analyzing peptides and proteins in order to elucidate fundamental biological processes that underlie both normal physiology and diseases that include AIDS, hepatitis C infection, drug addiction, chronic pain, cancer, bacterial infection, typ-2 diabetes, Alzheimer's disease, Parkinson's disease, sleeping sickness, and neuronal degeneration. The major subdivisions of the Resource are: Technological Research & Development (TR&D), Driving Biological Projects (DBPs), Collaboration & Service, Dissemination, and Education. Our three TR&D aims are: (1) Instrument development for increasing sensitivity and signal-to-noise, (2) Methodology development for elucidating 4-dimensional cellular interactomes and (3) Methodology development for elucidating complex high diversity systems including toxins and antibodies. Our DBPs will seek to (1) gain an understanding of broadly neutralizing anti-HIV antibodies; (2) develop improved methods for elucidating the structures of conotoxins as potential therapeutics; (3) profile neuropeptides in th CNS and peripheral circulation; (4) define the HIV-1 interactome; (5) define viral and host-derived proteins incorporated into HCV particles; (6) investigate a new tumor suppressor pathway in lymphoma; (7) elucidate the fine structure of the nuclear pore complex; (8) elucidate the biosynthetic pathway of S. aureus autoinducing peptide; (9) elucidate the biomolecular anatomy of inhibitory synapses; and (10) analyze the cellular response to DNA double-strand breaks. Our collaborations will seek to investigate: (1) African trypanosome proteins at the host-parasite interface and develop tools for proteomic analysis in trypanosomes; (2) autophagy protein complexes in animal models; (3) cyclin dependent kinase substrates; (4) mechanisms of nucleotide excision repair; (5) human L1 function; (7) mediator-dependent transcription; (8) islet amyloid formation; (9) structure of the transmembrane Type III secretion system needle; and (10) structure of the herpes simplex virus.

描述（由申请人提供）：建议在 40 至 44 年内继续运营和扩展生物大分子质谱分析国家资源的尖端能力。重点将放在开发用于分析肽的质谱技术和蛋白质，以阐明正常生理和疾病的基本生物过程，包括艾滋病、丙型肝炎感染、毒瘾、慢性疼痛、癌症、细菌感染、2型糖尿病、阿尔茨海默病、帕金森病、昏睡病和神经元变性。该资源的主要细分为：技术研究与开发（TR&D）、驱动生物项目（DBP）、协作与服务、传播和教育。我们的三个 TR&D 目标是：(1) 开发用于提高灵敏度和信噪比的仪器，(2) 开发用于阐明 4 维细胞相互作用组的方法学，以及 (3) 开发用于阐明复杂的高多样性系统（包括毒素和抗体）的方法学。我们的 DBP 将寻求 (1) 了解广泛中和抗 HIV 抗体； (2) 开发改进的方法来阐明芋螺毒素的结构作为潜在的治疗方法； (3) 分析中枢神经系统和外周循环中的神经肽； (4) 定义 HIV-1 相互作用组； (5) 定义掺入 HCV 颗粒中的病毒和宿主衍生蛋白； (6) 研究淋巴瘤新的抑癌通路； (7)阐明核孔复合体的精细结构； (8)阐明金黄色葡萄球菌自诱导肽的生物合成途径； (9) 阐明抑制性突触的生物分子解剖结构； (10)分析细胞对DNA双链断裂的反应。我们的合作将寻求研究：（1）宿主-寄生虫界面的非洲锥虫蛋白，并开发锥虫蛋白质组分析工具； (2)动物模型中的自噬蛋白复合物； (3) 细胞周期蛋白依赖性激酶底物； (4)核苷酸切除修复机制； (5)人类L1功能； (7)介导依赖性转录； (8)胰岛淀粉样蛋白形成； (9)跨膜III型分泌系统针的结构； (10)单纯疱疹病毒的结构。