Surface chemistry for high-contrast fluorescence kidney imaging

高对比度荧光肾脏成像的表面化学

基本信息

批准号：
8912470
负责人：
Jie Zheng
金额：
$ 33.33万
依托单位：
UNIVERSITY OF TEXAS DALLAS
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-09-01 至 2019-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8912470
关键词：
Address Adsorption Adult Behavior Biocompatible Blood Blood flow Caliber Cardiac Output Charge Chemistry Clinical Trials Contrast Media Detection Development Disease Engineering Exhibits Fluorescence Foundations Future Glutathione Gold Health Health Care Costs Hydrophobicity Image Imagery Imaging Techniques Investigation Kidney Kidney Diseases Kidney Neoplasms Kinetics Lead Life Ligands Liver Molecular Near-infrared optical imaging Operative Surgical Procedures Organ Organism Outcome Pathology Permeability Physiological Processes Plasma Play Polyethylene Glycols Positron-Emission Tomography Radioactive Tracers Renal carcinoma Renal clearance function Research Resistance Reticuloendothelial System Serum Proteins Skin Skin Tissue Solid Specificity Surface Surgeon Time Tissues Toxic effect Translating United States Urinary system Work clinical practice contrast imaging cost disease diagnosis drug development fluorescence imaging fluorophore image guided imaging probe improved in vivo kidney imaging kidney surgery meetings mouse model nanoparticle nanoprobe nanotoxicity pre-clinical single photon emission computed tomography success tool tumor uptake

项目摘要

DESCRIPTION In vivo fluorescence imaging, allowing the visualization of physiological processes in an intact living organism, has been an indispensable preclinical tool for fundamental understandings of disease pathology and development. More recently, it has also been translated into clinical trials to improve surgery outcomes and lower health-care costs. However, delivery of in vivo fluorescence imaging into preclinical kidney research and surgery remains highly challenging because of undesired in vivo behaviors such as rapid kidney clearance and high accumulation in background tissues of current NIR emitting contrast agents. As a result, in vivo kidney imaging remains heavily relied on high-cost radiological imaging techniques such as positron emission tomography (PET) and single-photon emission computed tomography (SPECT), which not only limits fundamental kidney research but also makes it challenging for surgeons to conduct imaging-guided kidney surgery in the future. The objective of this application to address these critical challenges by developing a class of renal clearable near infrared (NIR) emitting nanoprobes with high kidney-targeting specificity, tunable kidney retention time and minimum accumulation in normal background tissues and reticuloendothelial system (RES) organs, so that both noninvasive and invasive kidney imaging can be achieved at high contrast with desired detection time window.

描述体内荧光成像允许完整活体生物体中生理过程的可视化，已成为基本了解疾病病理学和发展不可或缺的临床前工具。最近，它还被转化为临床试验，以改善手术结果并降低医疗成本。然而，由于当前近红外发射造影剂的快速肾脏清除和背景组织中的高积累等不良体内行为，将体内荧光成像应用于临床前肾脏研究和手术仍然极具挑战性。因此，体内肾脏成像仍然严重依赖高成本的放射成像技术，例如正电子发射断层扫描（PET）和单光子发射计算机断层扫描（SPECT），这不仅限制了基础肾脏研究，而且给肾脏研究带来了挑战。外科医生将来可以进行影像引导的肾脏手术。本申请的目的是通过开发一类肾脏可清除的近红外（NIR）发射纳米探针来解决这些关键挑战，该探针具有高肾脏靶向特异性、可调节的肾脏保留时间以及在正常背景组织和网状内皮系统（RES）器官中的最小积累，从而可以在所需检测时间窗口的高对比度下实现非侵入性和侵入性肾脏成像。