Develop a Dual Functional Nanoprobe for Integration of Fluorescence microscopy wi

开发用于集成荧光显微镜的双功能纳米探针

基本信息

批准号：
8047401
负责人：
Jie Zheng
金额：
$ 21.04万
依托单位：
UNIVERSITY OF TEXAS DALLAS
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-07-01 至 2013-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8047401
关键词：
Address Affinity Binding Binding Proteins Biology Cell physiology Cells Chemical Structure Chemicals Complex Electron Microscopy Environment Event Fluorescence Fluorescence Microscopy Fluorescence Resonance Energy Transfer Fluorescent Probes Folic Acid Foundations Goals Homocysteine Homocystine Image Imaging Device Imaging Techniques In Situ In Vitro Label Life Ligands Location Measures Medicine Microscopic Microscopy Molecular Nanotechnology Nucleotide Biosynthesis Play Proteins Raman Spectrum Analysis Reaction Reporting Research Resolution Role Signal Transduction Silver Spectrum Analysis Structure Surface Techniques Time Work X-Ray Crystallography base bioimaging chemical reaction disease diagnosis folate-binding protein imaging probe in vivo interest nanoparticle nanoprobe nanoscience novel therapeutics particle programs protein complex receptor receptor binding response solid state success therapeutic development therapy development

项目摘要

DESCRIPTION (provided by applicant): The objective of the proposed application is to develop a dual functional probe which is detectable at single-particle level for both Raman and fluorescence microscopy, so that the strengths of each technique can be combined into a single imaging tool for tackling challenges in bioimaging. With this new imaging probe and tool, we plan to address a long-term bioimaging challenge that is real-time imaging of interactions between ligands and receptors at the molecular level inside live cells. Success of this proposed work will (1) provide a new imaging probe that is not only highly fluorescent and robust but can also report molecular information for ligands it labels; (2) offer a new imaging tool which integrates strengths of fluorescence microscopy and Raman spectroscopy; (3) enable us to correlate cellular dynamics of ligands with ligand-binding protein interactions at the chemical level inside live cells. The applications of these fluorescent and Raman probes are not limited to the proposed studies; they can also find applications in bioimaging as multiplexing and multifunctional probes to correlate fluorescence and Raman images with electron microscopy images at in vitro and in vivo level. PUBLIC HEALTH RELEVANCE: Ligand-receptor interactions play a pivotal role in regulating cellular functions and provide a foundation for disease diagnosis and new therapy development. However, comprehensive understanding of in vivo interactions between ligand and receptors at the molecular level remains highly challenging. Our research application aims to integrate fluorescence microscopy with Raman spectroscopy using a dual functional probe, so that we can simultaneously track and chemically image of ligand-receptor complexes at the single-cell level inside live cells. Success of this proposed work will (1) provide a new imaging probe that not only is highly fluorescent and robust but also can report molecular information of ligands it labels; (2) offer a new imaging tool which integrate strengths of fluorescence microscopy and Raman spectroscopy; (3) enable us to correlate cellular dynamics of ligands with ligand-receptor interactions at the chemical level inside live cells.

描述（由申请人提供）：拟议的应用的目的是开发一种双重功能探针，该探针可在拉曼和荧光显微镜下在单粒子水平上检测到，以便每种技术的优势都可以组合为单个成像工具，以应对生物映射中的挑战。借助这种新的成像探针和工具，我们计划应对长期生物成像挑战，这是对活细胞内部分子水平的配体和受体之间相互作用的实时成像。这项拟议工作的成功（1）将提供一种新的成像探针，不仅荧光且稳健，而且还可以报告IT标记的配体的分子信息；（2）提供了一种新的成像工具，该工具整合了荧光显微镜和拉曼光谱的强度；（3）使我们能够将配体的细胞动力学与活细胞内化学水平的配体结合蛋白相互作用相关联。这些荧光和拉曼探针的应用不限于拟议的研究。他们还可以在生物成像中找到应用作为多重探针和多功能探针，以将荧光和拉曼图像与电子显微镜图像在体外和体内水平相关联。公共卫生相关性：配体受体相互作用在调节细胞功能方面起着关键作用，并为疾病诊断和新的治疗发展提供了基础。但是，对分子水平配体和受体之间体内相互作用的全面理解仍然是高度挑战性的。我们的研究应用程序旨在使用双功能探针将荧光显微镜与拉曼光谱进行整合，以便我们可以在活细胞内的单细胞水平上同时跟踪配体受体复合物的化学图像和化学图像。这项提出的工作的成功（1）将提供一种新的成像探针，不仅是高度荧光和稳健的，而且还可以报告它标记的配体的分子信息；（2）提供了一种新的成像工具，该工具整合了荧光显微镜和拉曼光谱的强度；（3）使我们能够将配体的细胞动力学与活细胞内化学水平的配体 - 受体相互作用相关联。