Nonhuman Primate IVIS Spectrum Imaging System

非人类灵长类动物 IVIS 光谱成像系统

• 批准号: 8640351
• 负责人: Alice F Tarantal
• 金额: $ 40.5万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-15 至 2015-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8640351
• 关键词: Back; California; Center for Translational Science Activities; Communicable Diseases; Computer software; Detection; Development; Disease; Ensure; Environment; Fluorescence; Funding; Gene Transfer; Goals; Health; Health system; Human; Image; Imaging Techniques; Longevity; Longitudinal Studies; Manufacturer Name; Measurement; Modeling; Monkeys; Primates; Regenerative Medicine; Research; Research Personnel; Risk; Secure; Services; Signal Transduction; Source; Stem cell transplant; Structure; System; Tissue Engineering; United States National Institutes of Health; charge coupled device camera; data acquisition; gene therapy; in vivo imaging; meetings; nonhuman primate; novel; occupational health/safety; optical imaging; programs; transmission process

DESCRIPTION (provided by applicant): The California National Primate Research Center translational in vivo imaging program, which is a component of the Computational Imaging Core, provides state-of-the-art in vivo imaging services and expertise to NIH- funded investigators nationally. This established program includes unique opportunities for investigators to incorporate noninvasive imaging in their research programs, and to study monkey models of human health and disease. The goal of this proposal is to upgrade and replace an optical imaging system that will no longer be supported or serviced by the manufacturer/supplier placing many NIH studies in progress and planned at risk. The replacement IVIS Spectrum Imaging System will be maintained within this established core service structure, which has been highly effective in meeting the in vivo imaging needs of NIH-supported investigators nationwide. This imaging system will be located within a dedicated imaging suite in a secure environment, and with the required conditions for occupational health and safety for research with nonhuman primates. The IVIS Spectrum Imaging System can be used in both reflectance and transmission modes and includes a highly sensitive back-thinned, back-illuminated CCD camera, a broad band excitation source with filters for fluorescence measurements, selectable filters for multispectral detection, and software to control data acquisition, for spectral unmixin, 3D signal localization and quantification, and image display and analysis. In addition to ongoing longitudinal studies in monkeys focused on gene transfer/gene therapy, stem cell transplantation, regenerative medicine, tissue engineering, infectious diseases, and lifespan health, the system will serve as the platform for further development and application of these novel imaging techniques in monkeys, and span research domains. This replacement system will ensure ongoing and new research opportunities at the California National Primate Research Center can continue to meet the needs of NIH- funded investigators locally, regionally, and nationally that are focused on ways to better understand human health and disease.

描述（由申请人提供）：加州国家灵长类动物研究中心转化体内成像项目是计算成像核心的组成部分，为 NIH 资助的全国研究人员提供最先进的体内成像服务和专业知识。这一既定计划为研究人员提供了独特的机会，将无创成像纳入他们的研究计划，并研究人类健康和疾病的猴子模型。该提案的目标是升级和更换光学成像系统，该系统将不再由制造商/供应商提供支持或服务，从而使许多正在进行中和计划中的 NIH 研究面临风险。替换后的 IVIS 光谱成像系统将在这个已建立的核心服务结构内进行维护，该结构非常有效地满足了 NIH 支持的全国研究人员的体内成像需求。该成像系统将位于安全环境中的专用成像套件内，并具备非人类灵长类动物研究所需的职业健康和安全条件。 IVIS 光谱成像系统可用于反射和透射模式，包括高灵敏度背照式背照式 CCD 相机、带荧光测量滤光片的宽带激发源、用于多光谱检测的可选滤光片以及控制软件数据采集​​，用于光谱分解、3D 信号定位和量化以及图像显示和分析。除了正在进行的针对猴子的纵向研究（专注于基因转移/基因治疗、干细胞移植、再生医学、组织工程、传染病和寿命健康）之外，该系统还将作为进一步开发和应用这些新型成像技术的平台在猴子中，并且跨越研究领域。这一替代系统将确保加州国家灵长类动物研究中心正在进行的和新的研究机会能够继续满足美国国立卫生研究院资助的本地、区域和全国研究人员的需求，这些研究人员专注于更好地了解人类健康和疾病的方法。