Tissue Procurement Core

组织采购核心

• 批准号: 8728160
• 负责人: IDHALIZ FLORES
• 金额: $ 16.07万
• 依托单位: PONCE SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2017-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8728160
• 关键词: Affect; Basic Science; Biocompatible Materials; Biological; Biological Markers; Blood; Cancer Center; Caribbean region; Clinic; Clinical; Collaborations; Collection; Confidentiality of Patient Information; Country; DNA; Data; Development; Diagnostic; Ensure; Environment; Epigenetic Process; Florida; Fostering; Fresh Tissue; Funding; Future; Genes; Genetic; Genomics; Hispanics; Histological Techniques; Human; Immunohistochemistry; Individual; Information Storage; Institution; Institutional Review Boards; Island; Latin America; Life; Liquid substance; Malignant Neoplasms; Medicine; Methods; Mexican Americans; MicroRNAs; Minority; Molecular; NCI Center for Cancer Research; Normal tissue morphology; Paraffin; Patients; Pharmacogenomics; Population; Positioning Attribute; Predisposing Factor; Preparation; Prevalence; Procedures; Process; Proteomics; Protocols documentation; Public Health; Puerto Rican; Puerto Rico; RNA; Research; Research Infrastructure; Research Personnel; Research Support; Resources; Sampling; Serum; Single Nucleotide Polymorphism; Solid; Specimen; Techniques; Technology; Tissue Banking; Tissue Banks; Tissue Microarray; Tissue Procurements; Tissues; Translations; Tumor Tissue; anticancer research; base; biobank; cancer Biomedical Informatics Grid; cancer health disparity; cancer type; high standard; human tissue; medical schools; novel; prognostic; protein expression; repository; research study; response; tool; trend; tumor

The purpose of the PC at Ponce School of Medicine (PSM) is to facilitate the collection, processing, and storage of well-annotated human biospecimens for use in investigator-initiated translational cancer research. Throughout the past five years a solid infrastructure was put in place to develop the first tumor tissue bank in Puerto Rico, capable of the optimal collection, processing, and distribution of biological materials derived from the island's Hispanic population. Through this unique resource, which follows the same standard operational procedures (SOPs) established at the Tissue Core at MCC (and which are approved by our institutions' IRB committees), fresh tissues and high quality RNA are already available to support research at both institutions focusing on the molecular aspects and biological determinants of cancer health disparities involving Puerto Rican Hispanics living in Puerto Rico and Hispanics living in Florida. This facility is a valuable resource of high public health significance given that 1) Hispanics represent 15% of the US population (the fastest growing minority population), 2) most cancer health-disparity studies in the US have been conducted with Mexican-American populations, 3) there are differences in cancer trends and unique aspects associated to cancer health disparities among the different Hispanic populations, and 4) there is a scarcity of basic science-oriented cancer studies involving Puerto Ricans. Availability of biospecimens (normal/tumor tissue, serum/DNA) from this population will support much needed research on cancer health disparities and facilitate answering questions on gene-environment-culture interactions involving Hispanics. We propose to expand and further develop the existing Partnership TPC to: 1) extend the collection of biological materials to include paraffin blocks [to support studies on protein expression, microRNA, and the preparation of Tissue Microarrays (TMA)], and of blood [to support future genetic, epigenetic and proteomic analyses]; and 2) to provide support in basic histological techniques, immunohistochemistry (IHC) methods, and advanced pathological analyses to investigators at PSM. TMAs of cancer types that predominantly affect the Hispanic population will be developed utilizing the MCC Tissue Core infrastructure. A DNA/serum repository of Puerto Rican patient samples will create a unique resource that will allow investigators to take advantage of high-throughput genomics- and proteomics-based technologies also available at MCC. These resources will support studies of factors (e.g., single nucleotide polymorphisms [SNPs], ancestry contribution) predisposing individuals to cancer development, the impact of SNPs on patient response to therapy (pharmacogenomics), and the discovery of diagnostic or prognostic molecular and proteomic signatures. With the rise of individualized/personalized medicine and biomarker discovery efforts, establishing a tissue/DNA/serum repository would be a valuable resource that can be used for furthering our understanding of the molecular factors that predispose or protect the Puerto Rican population to cancer. The genetic basis of known disparities in Puerto Rican Hispanics, which include different prevalence rates of certain types of cancer, can only be uncovered through access to these biospecimens. This partnership is uniquely positioned to fulfill this need. In fact, the U56-funded TPC already provides basic and clinical Partnership investigators with a centralized mechanism to collect, store, and process human tissues following standardized protocols. Because multi-parameter characterization of collected biospecimens will be required for the translation of experimental research findings to the clinic, a decision was made early on to utilize caBIG¿'s biorepository management tool, caTissue Suite, to collect and store associated data, which will facilitate the correlation of important clinical-pathological data with research results, while preserving patient confidentiality. PSM is the only cancer research center in Puerto Rico, the Caribbean, or Latin America to be connected to caBIG. Extending this practice to multiple tumor types, normal tissues, and fluid specimens would represent a leap to the next level of sophistication. Efforts are in place to ensure that tissue banking and information storage procedures are conducted using the highest standards in the field and are also attuned to other biobanking efforts across the country via participation in GMaP/BMaP (MCC and PSM are partners in Region 3). It is envisioned that the U54-funded, Hispanic-specific TPC will foster increased collaborations among the Partnership institutions and with other institutions, and will facilitate the application of novel molecular techniques to solving cancer health disparities involving Puerto Rican Hispanics (living on the island or in the mainland) and other Hispanics living in the US. In addition, this TPC constitutes a valuable asset for the recruitment of cancer researchers to the PSM-MCC Partnership.

PONCE医学学院（PSM）的PC的目的是促进对研究人员发起的转化癌症研究的收集，加工和存储，以供应良好的人类生物测量。在过去的五年中，建立了一个稳定的基础设施，以开发波多黎各的第一个肿瘤组织库，该基础设施能够获得源自该岛西班牙裔人口的最佳收集，加工和分布。 Through this unique resource, which follows the same standard operational procedures (SOPs) established at the Tissue Core at MCC (and which are approved by our institutions' IRB committes), fresh tips and high quality RNA are already available to support research at both institutions focusing on the molecular aspects and biologic determiners of cancer health disparities involving Puerto Rican Hispanics living in Puerto Rico and Hispanics living in Florida.鉴于1）西班牙裔占美国人口的15％（少数人口最快），2）在美国进行了大多数癌症健康差异研究，设施是一种具有高公共健康意义的宝贵资源。波多黎各人。该人群中生物测量（正常/肿瘤组织，血清/DNA）的可用性将支持有关癌症健康分布的急需研究，并支持有关涉及西班牙裔的基因环境培养相互作用的回答问题。 我们建议将现有伙伴关系TPC扩展并进一步发展为：1）扩展生物材料的收集到包括石蜡块[支持蛋白质表达的研究，microRNA和组织微阵列（TMA）]（TMA）]和血液的制备[支持未来的遗传，表观遗传学和蛋白质组分析]； 2）为基本的组织学技术，免疫组织化学（IHC）方法提供支持，并向PSM的研究者提供了高级病理分析。 通过MCC组织核心基础设施，将开发主要影响西班牙裔人群的癌症类型的TMA。波多黎各患者样品的DNA/血清存储库将创建独特的资源，使研究人员能够利用MCC的高通量基因组学和基于蛋白质组学的技术。这些资源将支持对因素（例如，单核苷酸多态性[SNP]，祖先贡献）的研究，使个体对癌症发展，SNP对患者对治疗（药物基因组学）的影响以及发现诊断或蛋白质组学分子和蛋白质组学签名的影响。随着个性化/个性化医学和生物标志物发现工作的兴起，建立组织/DNA/血清存储库将是一种宝贵资源，可用于进一步，以进一步了解我们对易感或保护波多黎各人群癌症的分子因素的理解。波多黎各西班牙裔中已知差异的遗传基础，其中包括某些类型的癌症的不同患病率，只能通过获得这些生物测量来发现。这种合作伙伴关系是满足这一需求的独特定位。实际上，由U56资助的TPC已经为基本和临床合作伙伴的研究人员提供了一种集中的机制，可以按照标准化协议收集，存储和处理人体组织。由于将实验研究结果转换为诊所需要进行的多参数表征，因此很早就决定利用Cabig的生物设施管理工具，Catissue Suite，收集和存储相关的数据，这将支持重要的临床数据与研究结果的相关性，同时养育患者的信心。 PSM是波多黎各，加勒比海或拉丁美洲的唯一癌症研究中心，与Cabig连接。将这种练习扩展到多种肿瘤类型，正常组织和流体样本将代表到下一个努力的飞跃，以确保使用现场的最高标准进行组织库和信息存储程序，并通过参与GMAP/BMAP（MCC和PSM）在全国范围内参加其他生物群体（MCC和PSM）（MCC和PSM）。可以预见的是，由U54资助的西班牙裔特定的TPC将促进合伙机构和其他机构之间的合作，并将促进新型分子技术在涉及波多黎各Rican西班牙裔（在岛上或其他Hisspan）和其他Hisspanics中的癌症健康差异方面的应用。此外，该TPC构成了将癌症研究人员招募到PSM-MCC合作伙伴关系的宝贵资产。