Tissue Procurement Core

组织采购核心

基本信息

批准号：
8551288
负责人：
IDHALIZ FLORES
金额：
$ 16.75万
依托单位：
PONCE SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-01 至 2017-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8551288
关键词：
Affect Basic Science Biocompatible Materials Biological Biological Markers Blood Cancer Center Caribbean region Clinic Clinical Collaborations Collection Confidentiality of Patient Information Country DNA Data Development Diagnostic Ensure Environment Epigenetic Process Florida Fostering Fresh Tissue Funding Future Genes Genetic Genomics Hispanics Histological Techniques Human Immunohistochemistry Individual Information Storage Institution Institutional Review Boards Island Latin America Life Liquid substance Malignant Neoplasms Medicine Methods Mexican Americans MicroRNAs Minority Molecular NCI Center for Cancer Research Normal tissue morphology Paraffin Patients Pharmacogenomics Population Positioning Attribute Predisposing Factor Preparation Prevalence Procedures Process Proteomics Protocols documentation Public Health Puerto Rican Puerto Rico RNA Research Research Infrastructure Research Personnel Research Support Resources Sampling Serum Single Nucleotide Polymorphism Solid Specimen Techniques Technology Tissue Banking Tissue Banks Tissue Microarray Tissue Procurements Tissues Translations Tumor Tissue anticancer research base biobank cancer Biomedical Informatics Grid cancer health disparity cancer type high standard human tissue medical schools novel prognostic protein expression repository research study response tool trend tumor

项目摘要

The purpose of the PC at Ponce School of Medicine (PSM) is to facilitate the collection, processing, and storage of well-annotated human biospecimens for use in investigator-initiated translational cancer research. Throughout the past five years a solid infrastructure was put in place to develop the first tumor tissue bank in Puerto Rico, capable of the optimal collection, processing, and distribution of biological materials derived from the island's Hispanic population. Through this unique resource, which follows the same standard operational procedures (SOPs) established at the Tissue Core at MCC (and which are approved by our institutions' IRB committees), fresh tissues and high quality RNA are already available to support research at both institutions focusing on the molecular aspects and biological determinants of cancer health disparities involving Puerto Rican Hispanics living in Puerto Rico and Hispanics living in Florida. This facility is a valuable resource of high public health significance given that 1) Hispanics represent 15% of the US population (the fastest growing minority population), 2) most cancer health-disparity studies in the US have been conducted with Mexican-American populations, 3) there are differences in cancer trends and unique aspects associated to cancer health disparities among the different Hispanic populations, and 4) there is a scarcity of basic science-oriented cancer studies involving Puerto Ricans. Availability of biospecimens (normal/tumor tissue, serum/DNA) from this population will support much needed research on cancer health disparities and facilitate answering questions on gene-environment-culture interactions involving Hispanics. We propose to expand and further develop the existing Partnership TPC to: 1) extend the collection of biological materials to include paraffin blocks [to support studies on protein expression, microRNA, and the preparation of Tissue Microarrays (TMA)], and of blood [to support future genetic, epigenetic and proteomic analyses]; and 2) to provide support in basic histological techniques, immunohistochemistry (IHC) methods, and advanced pathological analyses to investigators at PSM. TMAs of cancer types that predominantly affect the Hispanic population will be developed utilizing the MCC Tissue Core infrastructure. A DNA/serum repository of Puerto Rican patient samples will create a unique resource that will allow investigators to take advantage of high-throughput genomics- and proteomics-based technologies also available at MCC. These resources will support studies of factors (e.g., single nucleotide polymorphisms [SNPs], ancestry contribution) predisposing individuals to cancer development, the impact of SNPs on patient response to therapy (pharmacogenomics), and the discovery of diagnostic or prognostic molecular and proteomic signatures. With the rise of individualized/personalized medicine and biomarker discovery efforts, establishing a tissue/DNA/serum repository would be a valuable resource that can be used for furthering our understanding of the molecular factors that predispose or protect the Puerto Rican population to cancer. The genetic basis of known disparities in Puerto Rican Hispanics, which include different prevalence rates of certain types of cancer, can only be uncovered through access to these biospecimens. This partnership is uniquely positioned to fulfill this need. In fact, the U56-funded TPC already provides basic and clinical Partnership investigators with a centralized mechanism to collect, store, and process human tissues following standardized protocols. Because multi-parameter characterization of collected biospecimens will be required for the translation of experimental research findings to the clinic, a decision was made early on to utilize caBIG¿'s biorepository management tool, caTissue Suite, to collect and store associated data, which will facilitate the correlation of important clinical-pathological data with research results, while preserving patient confidentiality. PSM is the only cancer research center in Puerto Rico, the Caribbean, or Latin America to be connected to caBIG. Extending this practice to multiple tumor types, normal tissues, and fluid specimens would represent a leap to the next level of sophistication. Efforts are in place to ensure that tissue banking and information storage procedures are conducted using the highest standards in the field and are also attuned to other biobanking efforts across the country via participation in GMaP/BMaP (MCC and PSM are partners in Region 3). It is envisioned that the U54-funded, Hispanic-specific TPC will foster increased collaborations among the Partnership institutions and with other institutions, and will facilitate the application of novel molecular techniques to solving cancer health disparities involving Puerto Rican Hispanics (living on the island or in the mainland) and other Hispanics living in the US. In addition, this TPC constitutes a valuable asset for the recruitment of cancer researchers to the PSM-MCC Partnership.

庞塞医学院 (PSM) 的 PC 的目的是促进注释良好的人类生物样本的收集、处理和存储，以用于研究者发起的转化癌症研究。在过去的五年中，建立了坚实的基础设施。开发波多黎各第一个肿瘤组织库，能够通过这种独特的资源，以最佳方式收集、处理和分配来自该岛西班牙裔人口的生物材料，并遵循与波多黎各建立的相同标准操作程序（SOP）。组织核心在 MCC（并经我们机构的 IRB 委员会批准），新鲜组织和高质量 RNA 已可用于支持两个机构的研究，重点关注居住在波多黎各的波多黎各西班牙裔癌症健康差异的分子方面和生物决定因素鉴于 1) 西班牙裔人口占美国人口的 15%（增长最快的少数族裔人口），2) 美国大多数癌症健康差异研究均已进行，该设施是具有高度公共卫生意义的宝贵资源。进行与墨西哥裔美国人，3) 不同西班牙裔人群的癌症趋势和与癌症健康差异相关的独特方面存在差异，4) 缺乏涉及波多黎各人的基础科学癌症研究（正常。来自该人群的肿瘤组织、血清/DNA）将支持急需的癌症健康差异研究，并有助于回答涉及西班牙裔的基因-环境-文化相互作用的问题。我们建议扩大和进一步发展现有的伙伴关系 TPC，以：1) 扩大生物材料的收集范围，包括石蜡块 [以支持蛋白质表达、microRNA 和组织微阵列 (TMA) 的制备研究] 和血液 [ 2) 为 PSM 的研究人员提供基本组织学技术、免疫组织化学 (IHC) 方法和高级病理学分析方面的支持。主要影响西班牙裔人群的癌症类型的 TMA 将利用 MCC 组织核心基础设施进行开发，波多黎各患者样本的 DNA/血清存储库将创建一个独特的资源，使研究人员能够利用高通量基因组学和蛋白质组学。 MCC 还提供基于基因的技术，这些资源将支持对癌症发展的诱发因素（例如，单核苷酸多态性 [SNP]、血统贡献）、个体影响的研究。关于患者对治疗的反应（药物基因组学）的 SNP，以及诊断或预后分子和蛋白质组学特征的发现，随着个体化/个体化医学和生物标志物发现工作的兴起，建立组织/DNA/血清存储库将是一种宝贵的资源。波多黎各西班牙裔已知差异的遗传基础，其中包括某些类型癌症的不同患病率，可用于进一步了解导致波多黎各人口罹患癌症的分子因素。事实上，U56 资助的 TPC 已经为基础和临床合作研究人员提供了一个按照标准化协议收集、存储和处理人体组织的集中机制。由于将实验研究结果转化为临床需要对收集的生物样本进行多参数表征，因此很早就决定使用 caBIG¿ PSM 的生物样本库管理工具 caTissue Suite 用于收集和存储相关数据，这将促进重要临床病理数据与研究结果的关联，同时保护患者机密。PSM 是加勒比海地区波多黎各唯一的癌症研究中心。或拉丁美洲连接到 caBIG。将这种做法扩展到多种肿瘤类型、正常组织和液体样本将代表着为确保组织库和信息存储程序的复杂性迈出了新的一步。采用该领域的最高标准进行，并通过参与 GMaP/BMaP（MCC 和 PSM 是第 3 区的合作伙伴）与全国其他生物样本库工作相协调。预计 U54 资助的针对西班牙裔的 TPC。将促进伙伴关系机构之间以及与其他机构之间加强合作，并将促进新型分子技术的应用，以解决涉及波多黎各西班牙裔（居住在岛上或大陆）和其他西班牙裔生活的癌症健康差异此外，该 TPC 为 PSM-MCC 合作伙伴招募癌症研究人员提供了宝贵的资产。