Muscle/Bone Phenotyping Core

肌肉/骨骼表型核心

• 批准号: 8281057
• 负责人: SARAH L DALLAS
• 金额: $ 25.53万
• 依托单位: UNIVERSITY OF MISSOURI KANSAS CITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2017-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8281057
• 关键词: Actinin; Address; Age; Aging; Alkaline Phosphatase; Bone Tissue; Calvaria; Cell Culture Techniques; Conditioned Culture Media; DEXA; Data; Densitometry; Dinoprostone; Education; Enzymes; Fluorochrome; Gene Expression; Genes; Goals; Histocytochemistry; Histology; Immunohistochemistry; In Situ Hybridization; Instruction; Label; Lead; Mechanical Stimulation; Mechanics; Methods; Mus; Muscle; Muscle Fibers; Muscle function; Myosin Heavy Chains; Osteoblasts; Osteocytes; Osteopenia; Paraffin Embedding; Phenotype; Postdoctoral Fellow; Preparation; Procedures; Process; Protein Isoforms; Protocols documentation; Regulation; Research; Research Personnel; Research Support; Resolution; Resources; Roentgen Rays; Sampling; Signal Transduction; Staining method; Stains; Students; Techniques; Tissue Sample; Tissues; Training; Transgenic Mice; age effect; age related; aged; bone; bone cell; bone imaging; bone mass; cost effective; in vivo; mouse model; muscle form; programs; research study; response; skeletal tissue; young adult

instnictions): The goal of the Muscle/Bone Phenotyping Core is to support the research alms of all subprojects within the program project. The overall aim of the program project is to understand the mechanisms underiying crosstalk between muscle and bone that may contribute to the age related decline In muscle and bone mass and function. This global question is addressed by each subproject from a different perspective, including: the effects of muscle on osteoblast and osteocyte function with aging (subproject 1); osteocyte modulation of muscle function during aging (subproject 2); osteocyte control of osteoblast dynamics with aging (subproject 3); and effects of aging on osteocyte responses to mechanical stimulation (subproject 4). The Muscle/Bone Phenotyping Core will provide phenotyping support for all subprojects, which will include In vivo and ex vivo X-ray, densitometric and microCT analysis of skeletal tissues, histological preparation and staining of muscle and mineralized tissues, quantitative histomorphometry and dynamic bone and muscle histomorphometry, immunohistochemistry and in situ hybridization. These techniques are essential for all the subprojects in which muscle and bone phenotypes are being characterized as a function of age in transgenic mouse models with altered osteocyte or muscle function, as well as mice that have been subjected to mechanical loading. In addition to providing these sen/lces the core will provide standardized protocols for sample preparation and for all methods within the core to maintain consistency across the subprojects. The core will also act as a resource to provide training in the above techniques for students, postdocs and research personnel conducting research within subprojects 1, 2, 3 and 4. By centralizing these phenotyping methods within a single core, we will standardize these techniques across all the subprojects, accelerate the pace of the research, enhance the education of students and postdocs and enable the research to be completed in a more cost-effective manner. RELEVANCE (See instructions): This research core will provide centralized support for four subprojects within a program project whose goal is to understand the mechanisms underiying muscle and bone interactions that contribute to age-related decline in muscle and bone mass. The core will standardize methods for analysis of muscle and bone in the mouse models used in all projects. This research may lead to new treatments for osteopenia/sarcopenla.

指示）： 肌肉/骨骼表型核心的目标是支持该项目内所有子项目的研究资助 计划项目。该计划项目的总体目标是了解其背后的机制 肌肉和骨骼之间的串扰可能导致与年龄相关的肌肉和骨量下降 和功能。每个子项目从不同的角度解决这个全球性问题，包括： 随着衰老，肌肉对成骨细胞和骨细胞功能的影响（子项目 1）；骨细胞的调节 衰老过程中的肌肉功能（子项目2）；骨细胞对成骨细胞动力学随衰老的控制（子项目 3）；以及衰老对骨细胞对机械刺激反应的影响（子项目 4）。肌肉/骨骼 表型核心将为所有子项目提供表型支持，其中包括体内和离体 骨骼组织的 X 射线、光密度和 microCT 分析、肌肉的组织学制备和染色 和矿化组织，定量组织形态计量学和动态骨骼和肌肉组织形态计量学， 免疫组织化学和原位杂交。这些技术对于所有子项目都是必不可少的 转基因小鼠的哪些肌肉和骨骼表型被表征为年龄的函数 骨细胞或肌肉功能改变的模型，以及经过机械训练的小鼠 加载中。除了提供这些服务之外，核心还将提供样本的标准化协议 准备和核心内的所有方法，以保持子项目之间的一致性。核心将 还充当为学生、博士后和研究人员提供上述技术培训的资源 在子项目 1、2、3 和 4 中进行研究的人员。通过集中这些表型分析方法 在单个核心内，我们将在所有子项目中标准化这些技术，加快 研究，加强学生和博士后的教育，使研究能够在 更具成本效益的方式。 相关性（参见说明）： 该研究核心将为一个计划项目中的四个子项目提供集中支持，其目标 是了解导致年龄相关的肌肉和骨骼相互作用的机制 肌肉和骨量下降。该核心将使肌肉和骨骼分析方法标准化 所有项目中使用的鼠标模型。这项研究可能会带来骨质减少/肌少症的新治疗方法。