Leica Stellaris 8 Confocal Microscope System

Leica Stellaris 8 共焦显微镜系统

• 批准号: 10431037
• 负责人: SARAH L DALLAS
• 金额: $ 59.93万
• 依托单位: UNIVERSITY OF MISSOURI KANSAS CITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-15 至 2023-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10431037
• 关键词: 3-Dimensional; Age related macular degeneration; Age-Related Osteoporosis; Aging; Antineoplastic Agents; Biological Sciences; Bone Tissue; Calcium Signaling; Cell physiology; Cells; Child; Childhood; Cleft Palate; Communication; Computers and Advanced Instrumentation; Confocal Microscopy; Core Facility; Dental Research; Detection; Development; Developmental Biology; Disease; Drug Delivery Systems; Elements; Engineering; Exercise; Fibrosis; Fluorescence; Fluorescence Microscopy; Fluorescence Recovery After Photobleaching; Funding; Genomics; Glaucoma; Health; Heart Contractilities; Hospitals; Image; Image Enhancement; Jaw; Lasers; Liquid substance; Malignant Neoplasms; Mediating; Medicine; Microscope; Minerals; Minor; Mitochondria; Modeling; Molecular; Molecular Biology; Monitor; Morphogenesis; Muscle; Musculoskeletal; Nerve Degeneration; Optics; Osteocytes; Pharmacy facility; Research; Research Support; Resolution; Resource Sharing; Scanning; School Dentistry; Signal Transduction; Specimen; System; Technology; Three-Dimensional Imaging; Tissue Engineering; Tissue imaging; Tissues; United States National Institutes of Health; Vision; Vision research; aged; bone; cellular imaging; confocal imaging; craniofacial; extracellular vesicles; imaging capabilities; instrument; live cell imaging; loss of function; materials science; mechanotransduction; mouse model; multiphoton microscopy; nano; nanoparticle; prevent; programs; relating to nervous system; restorative dentistry; sarcopenia; spectrograph; yeast genetics

7. Project Summary/Abstract Our ability to understand cellular function in health and disease from the macro to micro to nano and even to the atomic scale depend on the capabilities of state-of-the-art advanced instrumentation. The NIH funded projects described in this S10 application require enhanced imaging resolution and multi-spectral capabilities for their fluorescence microscopy applications to advance to the next level. A Leica Stellaris 8 laser scanning confocal microscope is requested and will be located in the UMKC Confocal and Multiphoton Microscopy Core Facility. This instrument will replace and upgrade our current Leica TCS II Sp5 confocal microscope that operates as a shared resource to support the research programs of a core group of NIH funded major users as well as minor and other users in the UMKC Schools of Dentistry, Pharmacy, Medicine, Biological Sciences, Computing and Engineering, and the nearby Children’s Mercy Hospital. The user group has a diverse research emphasis on musculoskeletal research, neural/vision research, developmental biology, craniofacial and dental research, molecular biology, yeast genetics, neurodegeneration, aging, cancer and drug delivery, materials science, and even petrochemical research. To accommodate the wide-ranging project applications, the instrument will be configured for confocal microscopy on fixed cell and tissue specimens, and for live cell imaging, multispectral imaging, fluorescence recovery after photobleaching (FRAP), optical sectioning/3D imaging, and reflection imaging. Three of the projects employing this technology are examining mechanisms of signaling crosstalk between muscle and bone that may regulate age related osteoporosis and sarcopenia. The projects are using live cell imaging to monitor mitochondrial function and extracellular vesicle-mediated muscle-bone communication and are using 3D confocal imaging to assess the cellular and structural organization of bone in aged gain and loss of function mouse models with and without exercise. One project is using live cell imaging to examine mechanisms controlling calcium signaling to prevent neurodegeneration in early stage glaucoma and two projects are developing drug delivery and tissue engineering approaches to treat age related macular degeneration. Other projects are focused on molecular and cellular mechanisms of cleft palate formation, heart contractile function and calcium signaling, developmental jaw morphogenesis, single cell and spatial genomics in pediatric bone tissues, finite element and fluid dynamic modeling of osteocyte mechanotransduction and nanoparticle systems for drug delivery in cancer and fibrosis. The Stellaris 8 confocal system will accelerate the progress of these projects by providing an exceptional level of resolution and sensitivity that is needed for 3D cell and tissue imaging and live cell imaging in mineralized and other tissues. The multi-spectral imaging capabilities of the Stellaris 8, the versatility of its excitation and detection capabilities, its enhanced sensitivity and its TauSense/ fluorescence lifetime technology are critical to advance this research to the next level, and will allow for expansion of our capabilities for multiplexing, 3D spatial mapping and cleared tissue imaging. Acquisition of this technology will accelerate discoveries in musculoskeletal health, development, aging, vision, dental restorations, and drug delivery.

7. 项目总结/摘要 我们从宏观到微观到纳米乃至微观层面理解健康和疾病中的细胞功能的能力 原子尺度取决于最先进的先进仪器的能力，美国国立卫生研究院资助的项目。 此 S10 应用中描述的应用需要增强的成像分辨率和多光谱功能 将荧光显微镜应用提升到新的水平 Leica Stellaris 8 激光扫描共焦。 需要显微镜，并将位于 UMKC 共焦和多光子显微镜核心设施中。 仪器将取代和升级我们当前的 Leica TCS II Sp5 共焦显微镜，该显微镜作为共享设备运行 支持 NIH 资助的主要用户以及次要用户和其他用户的核心小组的研究计划的资源 UMKC 牙科学院、药学院、医学学院、生物科学学院、计算机与工程学院的用户，以及 附近的儿童慈善医院的用户群体有不同的研究重点，重点是肌​​肉骨骼研究， 神经/视觉研究、发育生物学、颅面和牙科研究、分子生物学、酵母遗传学、 神经退行性疾病、衰老、癌症和药物输送、材料科学，甚至石油化学研究。 为了适应广泛的项目应用，该仪器将配置为共焦显微镜 固定细胞和组织标本，并用于活细胞成像、多光谱成像、荧光恢复后 其中三个项目采用了光漂白 (FRAP)、光学切片/3D 成像和反射成像。 技术正在研究肌肉和骨骼之间可能调节年龄相关信号串扰的机制 该项目正在使用活细胞成像来监测线粒体功能和骨质疏松症。 细胞外囊泡介导的肌肉骨通讯，并使用 3D 共焦成像来评估细胞 以及有和没有运动的老年功能获得和丧失小鼠模型中的骨结构组织。 该项目正在使用活细胞成像来检查控制钙信号传导以预防神经退行性变的机制 在青光眼的早期阶段，两个项目正在开发药物输送和组织工程方法来治疗 其他项目侧重于腭裂的分子和细胞机制。 形成、心脏收缩功能和钙信号传导、颌骨发育形态发生、单细胞和空间 儿科骨组织基因组学、骨细胞机械传导的有限元和流体动力学建模以及 用于癌症和纤维化药物输送的纳米颗粒系统将加速这一过程。 通过提供 3D 细胞和所需的卓越水平的分辨率和灵敏度来推动这些项目的进展 矿化组织和其他组织的组织成像和活细胞成像。 Stellaris 8，其激发和检测功能的多功能性、增强的灵敏度及其 TauSense/ 荧光寿命技术对于将这项研究推进到一个新的水平至关重要，并将允许扩大 我们的多路复用、3D 空间测绘和透明组织成像能力将得到提升。 加速肌肉骨骼健康、发育、衰老、视力、牙齿修复和药物输送方面的发现。