TRAINING IN FOURIER TRANSFORM ION CYCLOTRON RESONANCE MASS SPECTROMETRY

傅里叶变换离子回旋共振质谱培训

基本信息

批准号：
8365521
负责人：
CHI-WEI LIN
金额：
$ 0.77万
依托单位：
BOSTON UNIVERSITY MEDICAL CAMPUS
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-06-01 至 2012-08-09
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8365521
关键词：
Biochemistry Biology Boston Chemistry Complex Custom Dissociation Doctor of Philosophy Electrons England Fourier Transform Fourier transform ion cyclotron resonance Funding Germany Grant Infusion procedures Ions Korea Laboratory Personnel Left Mass Spectrum Analysis Medicine Methods National Center for Research Resources Performance Positioning Attribute Postdoctoral Fellow Principal Investigator Proteins Reporting Research Research Infrastructure Research Institute Research Personnel Research Project Grants Resources SHFM1 gene Sampling Science Scientist Source Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Students Techniques Training United States National Institutes of Health Universities Update Work base cost deamidation falls graduate student instrument mass spectrometer medical schools professor programs research study tandem mass spectrometry

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. One of the objectives of this program is to train new graduate students and professional scientific researchers in new techniques. One such technique which we have focused on is Fourier transform mass spectrometry. Since the departure of Dr. Peter O'Connor in Jan 2009 to become a tenured full professor in the Department of Chemistry at the University of Warwick, Coventry, England, Dr. Cheng Lin has assumed responsibility for training in FTMS, and FTMS-based research projects. He is now a research assistant professor in the Department of Biochemistry at Boston University School of Medicine, and the Assistant Director of the Resource. Updates for the reporting period of April 2010 - March 2011: A graduate student from the FTMS research group, Xiaojuan Li, defended her Ph. D. thesis, "Mechanism of electron capture dissociation and the application to protein deamidation", in July 2010, and subsequently joined Prof. Anderson's group as a postdoctoral research associate at Yale University. A former postdoc, Dr. Konstantin Aizikov left in December 2010 to take a research scientist position at Thermo Fisher Scientific at Bremen in Germany. In December 2010, Dr. Sang Yun Han from Korea Research Institute of Standards and Science joined the FTMS group to begin his 9-month sabbatical. He will be working primarily on developing tandem mass spectrometry methods for MALDI-generated ions using the custom-built MALDI-FTICR-MS instrument. In fall 2010, the IR option has been successfully set up on the 12 T Bruker solariX FT-ICR instrument, which enabled performance of a wide variety of experiments, including activated ion (AI)-ECD, post-ETD complex activation, and IRMPD. An Advion Nanomate has also been recently set up to allow automated infusion of sample into the mass spectrometer and a more efficient and stable spray. A number of graudate students and research scientists have been properly trained to operate this high-performance instrument, either independently or under the guidance of senior laboratory personnel.

该子项目是利用资源的众多研究子项目之一由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持并且子项目的主要研究者可能是由其他来源提供的，包括其他 NIH 来源。子项目可能列出的总成本代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。该项目的目标之一是培训新研究生和专业科学研究人员掌握新技术。我们关注的一项此类技术是傅里叶变换质谱法。自 Peter O'Connor 博士于 2009 年 1 月离职成为英国考文垂华威大学化学系终身正教授以来，Cheng Lin 博士一直负责 FTMS 的培训，以及基于 FTMS 的培训。研究项目。他现在是波士顿大学医学院生物化学系的研究助理教授，兼资源部助理主任。 2010年4月至2011年3月报告期的更新： FTMS课题组研究生李晓娟于2010年7月通过了博士论文“电子捕获解离机制及其在蛋白质脱酰胺中的应用”，随后加入Anderson教授课题组担任博士后研究员在耶鲁大学。 Konstantin Aizikov 博士曾是一名博士后，于 2010 年 12 月离职，前往德国不来梅 Thermo Fisher Scientific 担任研究科学家。 2010年12月，韩国标准科学研究所的Sang Yun Han博士加入FTMS小组，开始了为期9个月的休假。他将主要致力于使用定制的 MALDI-FTICR-MS 仪器开发 MALDI 生成离子的串联质谱方法。 2010 年秋季，IR 选项已在 12 T Bruker SolariX FT-ICR 仪器上成功设置，可进行多种实验，包括活化离子 (AI)-ECD、ETD 后复合物活化和 IMPD 。最近还设置了 Advion Nanomate，可以将样品自动注入质谱仪并实现更高效、更稳定的喷雾。许多研究生和研究科学家都接受过适当的培训，可以独立或在高级实验室人员的指导下操作这种高性能仪器。