ALGNATE BIOSYNTHESIS -- A COMPONENT OF BIOFILM FORMATION

海藻酸盐生物合成——生物膜形成的一个组成部分

• 批准号: 8363379
• 负责人: PATRICIA LYNNE HOWELL
• 金额: $ 0.52万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363379
• 关键词: Alginates; Anabolism; Chronic; Complex; Crystallography; Funding; Goals; Grant; Infection; Light; Lung; Membrane; Methods; Microbial Biofilms; Multiprotein Complexes; National Center for Research Resources; Phenotype; Polysaccharides; Principal Investigator; Pseudomonas aeruginosa; Research; Research Infrastructure; Resources; Source; Structure; Synchrotrons; United States National Institutes of Health; cell envelope; cost; cystic fibrosis patients; member; mucoid; novel; pathogen; porin

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Pseudomonas aeruginosa is an opportunistic pathogen that causes chronic biofilm infections in the lungs of cystic fibrosis patients. During colonization of the lung, P. aeruginosa converts to a mucoid phenotype characterized by the overproduction of the exopolysaccharide alginate. Exopolysaccharides are the major component of bacterial biofilms and are believed to be produced and secreted by multiprotein complexes spanning the cell envelope. The exopolysaccharide is believed to be secreted across the outer membrane by a porin, AlgE. Understanding the structure of AlgE and how it facilitates transport of the polysaccharide and interacts with other members of the secretion complex will be critical to our long term goal of developing novel methods to disrupt exoplysaccharide biosynthesis and therefore biofilm formation.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 铜绿假单胞菌是一种机会性病原体，可引起囊性纤维化患者肺部慢性生物膜感染。 在肺部定植期间，铜绿假单胞菌转化为粘液样表型，其特征在于胞外多糖藻酸盐的过量产生。 胞外多糖是细菌生物膜的主要成分，被认为是由跨越细胞膜的多蛋白复合物产生和分泌的。据信胞外多糖是由孔蛋白 AlgE 跨过外膜分泌的。了解 AlgE 的结构以及它如何促进多糖的运输以及与分泌复合物的其他成员相互作用对于我们开发新方法来破坏外多糖生物合成并从而破坏生物膜形成的长期目标至关重要。