ALGNATE BIOSYNTHESIS

海藻酸盐生物合成

• 批准号: 8170590
• 负责人: PATRICIA LYNNE HOWELL
• 金额: $ 0.29万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170590
• 关键词: Alginates; Anabolism; Chronic; Complex; Computer Retrieval of Information on Scientific Projects Database; Crystallography; Funding; Goals; Grant; Infection; Institution; Lung; Membrane; Methods; Microbial Biofilms; Multiprotein Complexes; Phenotype; Polysaccharides; Pseudomonas aeruginosa; Research; Research Personnel; Resources; Source; Structure; United States National Institutes of Health; cell envelope; cystic fibrosis patients; member; mucoid; novel; pathogen; porin

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Pseudomonas aeruginosa is an opportunistic pathogen that causes chronic biofilm infections in the lungs of cystic fibrosis patients. During colonization of the lung, P. aeruginosa converts to a mucoid phenotype characterized by the overproduction of the exopolysaccharide alginate. Exopolysaccharides are the major component of bacterial biofilms and are believed to be produced and secreted by multiprotein complexes spanning the cell envelope. The exopolysaccharide is believed to be secreted across the outer membrane by a porin, AlgE. Understanding the structure of AlgE and how it facilitates transport of the polysaccharide and interacts with other members of the secretion complex will be critical to our long term goal of developing novel methods to disrupt exoplysaccharide biosynthesis and therefore biofilm formation.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 铜绿假单胞菌是一种机会性病原体，可引起囊性纤维化患者肺部慢性生物膜感染。 在肺部定植期间，铜绿假单胞菌转化为粘液样表型，其特征在于胞外多糖藻酸盐的过量产生。 胞外多糖是细菌生物膜的主要成分，被认为是由跨越细胞膜的多蛋白复合物产生和分泌的。据信胞外多糖是由孔蛋白 AlgE 跨过外膜分泌的。了解 AlgE 的结构以及它如何促进多糖的运输以及与分泌复合物的其他成员相互作用对于我们开发新方法来破坏外多糖生物合成并从而破坏生物膜形成的长期目标至关重要。