ALGINATE BIOSYNTHESIS PATHWAY

海藻酸盐生物合成途径

• 批准号: 7726218
• 负责人: PATRICIA LYNNE HOWELL
• 金额: $ 0.37万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-18 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7726218
• 关键词: Alginates; Anabolism; Antibiotics; Bacteria; Biological Models; Cells; Computer Retrieval of Information on Scientific Projects Database; Cystic Fibrosis; Disinfectants; Funding; Goals; Grant; Host Defense Mechanism; Infection; Infectious Agent; Institution; Lung; Medical; Microbial Biofilms; Modification; Nucleosides; Organism; Pathway interactions; Personal Satisfaction; Play; Polymers; Polysaccharides; Process; Production; Proteins; Pseudomonas aeruginosa; Research; Research Personnel; Resources; Role; Source; Staging; Techniques; United States National Institutes of Health; pathogen; polymerization; structural biology; sugar

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Biosynthesis and export of Pseudomonas aeruginosa alginate: Bacteria growing in matrix-embedded biofilms are a significant medical and environmental problem because once the biofilm is established the bacteria are difficult to eradicate. Embedded in a biofilm, the bacteria are protected from the natural defense mechanisms of the host organism and are tolerant of antibiotics and disinfectants. The biofilm matrix is composed predominantly of polysaccharide and it is this exopolysaccharide 'coating' that plays a key role in protecting the bacteria. Formation of the exopolysaccharide is a multi-step process, which requires the production of nucleoside diphospho-sugar precursors, assembly of these sugar precursors into a polymer, post-polymer modification of the polysaccharide, and finally, export and release of the polymer from the cell. While the biosynthesis of the activated sugar precursor is well defined, less is known about the later stages of the process. We have chosen the opportunistic pathogen Pseudomonas aeruginosa, a paradigm organism for studying bacterial biofilms, as a model system to study bacterial exopolysaccharide polymerization and export. Pseudomonas aeruginosa is the primary infectious agent in lung infections of people suffering from Cystic Fibrosis (CF). Using a combination of microbiological and structural biology techniques our goal is to understand the roles of the proteins involved in alginate biosynthesis in Pseudomonas aeruginosa.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 铜绿假单胞菌海藻酸盐的生物合成和出口： 在基质嵌入的生物膜中生长的细菌是一个重大的医学和环境问题，因为一旦形成生物膜，细菌就很难根除。 嵌入生物膜中的细菌受到保护，免受宿主生物体自然防御机制的影响，并且能够耐受抗生素和消毒剂。 生物膜基质主要由多糖组成，正是这种胞外多糖“涂层”在保护细菌方面发挥着关键作用。胞外多糖的形成是一个多步骤的过程，需要生产核苷二磷糖前体，将这些糖前体组装成聚合物，对多糖进行聚合物后修饰，最后将聚合物从胞外导出和释放。细胞。虽然活化糖前体的生物合成已被明确定义，但对该过程的后期阶段知之甚少。 我们选择机会病原体铜绿假单胞菌（一种研究细菌生物膜的范例生物）作为研究细菌胞外多糖聚合和输出的模型系统。铜绿假单胞菌是囊性纤维化 (CF) 患者肺部感染的主要传染源。 结合微生物学和结构生物学技术，我们的目标是了解铜绿假单胞菌中藻酸盐生物合成所涉及的蛋白质的作用。